关键词: 黄瓜, SSH, 胚胎发育, cDNA文库

Abstract:

【Objective】 The aim of this experiment is to investigate the embryo development-related genes in cucumber, and study its molecular mechanism.【Method】 A subtracted cDNA library was constructed by using Suppression Subtractive Hybridzation (SSH). 【Result】 A total of 97 positive clones were screened using reverse Northern blot hybridization. After the removal of duplication and sequencing of low-quality sequences, 93 UniESTs were identified, including 86 singletons and 7 contigs. The 93 unique ESTs were used to BLAST in the GenBank non-redundant database. There were 83 ESTs were found matching homologous sequences, 10 ESTs homologous sequence were not found, presumably may be new genes. 【Conclusion】 Many of these ESTs were highly homologous with the heat shock proteins of Arabidopsis, rice and corn. Application of the GeneOntology functional classification, cell defense and metabolic processes in early embryos accounted for the main location. Cell defense and anti-osmotic stress function is very important in late embryos.

Key words: cucumber, SSH, embryonic development, cDNA library