关键词: 猪, IGF2R, 单核苷酸多态性(SNP), 遗传印记

Abstract:

【Objective】 In this study, single nucleotide polymorphism (SNP) was found in IGF2R exons and used as marker to investigate the imprinting pattern of IGF2R in swine. 【Method】 Gene polymorphism of swine IGF2R was detected from 243 pigs of three breeds by PCR-SSCP. Imprinting patterns of IFG2R exon 48 were analyzed in organs of three heterozygous piglets and one hog by RT-PCR-RFLP/SSCP. 【Result】 One SNP(a G→A transition) was identified in exon 48 of IGF2R , located at the 384th nucleotide (5→3),forming a Nsp I restriction site. Maternal expression of IGF2R exon 48 was observed in the main organs of four heterozygous pigs. 【Conclusion】 IGF2R exon 48 was maternally expressed and paternally imprinted in most tissues of piglets and hogs.

Key words: swine, IGF2R, SNP, genomic imprinting