中国农业科学 ›› 2009, Vol. 42 ›› Issue (12): 4301-4307 .doi: 10.3864/j.issn.0578-1752.2009.12.023

• 园艺 • 上一篇    下一篇

番茄砧木对南方根结线虫抗性鉴定

贾双双,高荣广,徐 坤

  

  1. (山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部园艺作物生物学重点开放实验室
  • 收稿日期:2009-03-05 修回日期:2009-06-16 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 徐坤

Screening and Evaluation of Tomato Rootstocks for Resistance to Meloidogyne incognita

JIA Shuang-shuang, GAO Rong-guang, XU Kun
  

  1. (山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部园艺作物生物学重点开放实验室
  • Received:2009-03-05 Revised:2009-06-16 Online:2009-12-10 Published:2009-12-10
  • Contact: XU Kun

摘要:

【目的】研究确定准确鉴定评价番茄砧木抗南方根结线虫侵染能力的方法,筛选抗性砧木材料。【方法】通过测定16个盆栽番茄砧木幼苗接种南方根结线虫50 d时的相对生长量及相关抗病指标,利用系统聚类分析和隶属函数运算相结合的方法进行综合分析。【结果】南方根结线虫侵染对不同番茄砧木幼苗各器官相对生长量、相关抗病指标及其变异系数的影响显著不同,其中生长量的变异系数以相对根鲜质量较大,为20.20%,相对茎粗较小,仅6.22%。采用相对生长量及抗病指标分别进行系统聚类的结果与利用根结指数的聚类结果一致,但与病情指数的聚类结果存在些微差异。根据综合指标隶属函数运算,确定了供试番茄砧木抗南方根结线虫的顺序。【结论】采用多指标综合聚类分析较单一指标聚类分析更能准确划分番茄砧木抗根结线虫的类群,通过聚类分析和隶属函数相结合的方法,确定Bパリア为免疫材料,BESUPA、坂砧2号、砧木606、砧木002、砧木001、TMS-150、サポ-ト、MIKADO、砧木401为抗病材料,アンカ-T为耐病材料,坂砧1号、北京1号为感病材料,128、BF兴津101、LS-89为高感材料。

关键词: 番茄砧木, 南方根结线虫, 相对生长量, 抗病指标, 聚类分析, 隶属函数分析

Abstract:

【Objective】 In order to screen the resistant tomato rootstocks, the screening method and the resistance to Meloidogyne incognita were studied in the experiment. 【Method】 The relative growth rate and relevant disease resistant index of 16 tomato rootstock seedlings planted in pots and inoculated with M. incognita were measured after 50 days, moreover cluster analysis and subordinate function were used in the experiment. 【Result】 The results showed that different relative growth rates of different organs, different disease resistant indexes of 16 tomato rootstock seedlings and their different coefficient variances resulted from the infection of M. incognita were different, in which the coefficient variances of relative root fresh mass of five relative growth rate was 20.20%, the highest, but that of relative stem diameter was only 6.22%. The results of clustering analysis with the relative growth rate and the disease resistant index were same as GI, but be a little different from the results of clustering analysis with DI. By the method of subordinate function, the sequence of resistance from strong to weak successively was determined.【Conclusion】 The result of cluster analysis with multiple indexes and total indexes was more accurate than other single index for the resistant class of tomato rootstocks to M. incognita. By the method of clustering analysis and subordinate function, Baliya is determined as an immune material; BESUPA, Banzhen No.2, Rootstock 606, Rootstock002, Rootstock001, TMS-150, SUPPORT, MIKADO, Rootstock 401 are resistant materials; Anka-T is a disease tolerant material; Banzhen No.1 and Beijing No.1 are sensitive materials; 128, BF xingjing 101, and LS-89 are highly sensitive materials.

Key words: tomato rootstock, Meloidogyne incognita, relative growth rate, disease resistant index, clustering analysis, subordinate function analysis