中国农业科学 ›› 2009, Vol. 42 ›› Issue (10): 3592-3599 .doi: 10.3864/j.issn.0578-1752.2009.10.0026

• 园艺 • 上一篇    下一篇

夜间低温对薄皮甜瓜果实糖积累及代谢相关酶活性的影响

郝敬虹,李天来,孟思达,赵 博,孙利萍

  

  1. (沈阳农业大学园艺学院/教育部设施园艺重点实验室/辽宁省设施园艺重点实验室)
  • 收稿日期:2009-01-07 修回日期:2009-06-30 出版日期:2009-10-10 发布日期:2009-10-10
  • 通讯作者: 李天来

Effects of Night Low Temperature on Sugar Accumulation and Sugar-Metabolizing Enzyme Activities in Melon Fruit

HAO Jing-hong, LI Tian-lai, MENG Si-da, ZHAO Bo, SUN Li-ping
  

  1. (沈阳农业大学园艺学院/教育部设施园艺重点实验室/辽宁省设施园艺重点实验室)
  • Received:2009-01-07 Revised:2009-06-30 Online:2009-10-10 Published:2009-10-10
  • Contact: LI Tian-lai

摘要:

【目的】探讨夜间低温胁迫下薄皮甜瓜果实糖代谢的变化规律。【方法】以薄皮甜瓜(Cucumis melo L.)‘玉美人’为试材,利用人工气候室模拟设施生产中的夜间低温胁迫环境,研究夜间低温对薄皮甜瓜果实糖含量及其代谢相关酶活性的影响。【结果】不同的夜间低温胁迫对果实糖含量的影响程度不同。夜间9℃处理3 d后显著降低了果糖和葡萄糖含量,在处理的中后期蔗糖、棉籽糖和水苏糖含量才显著降低而肌醇半乳糖苷无显著差异;同时,夜间9℃处理3 d后,果实中总糖含量极显著低于对照,且随处理时间的延长差异更大;夜间9℃处理3~12 d,果实中淀粉含量明显高于对照。夜间12℃处理对果实中可溶性糖含量无明显影响,但明显增加了淀粉含量,且后期差异极显著。从不同的夜间低温对果实内糖代谢相关酶活性的影响来看,夜间9℃处理增强了酸性转化酶(AI)、中性转化酶(NI)、碱性α-半乳糖苷酶活性,降低了蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)活性,差异均达到显著或极显著水平。夜间12℃处理对各种酶活性影响不大,差异不显著。【结论】夜间9℃处理严重影响薄皮甜瓜果实糖积累及其代谢相关酶活性;夜间12℃处理对果实糖积累及其代谢相关酶活性无明显影响,夜间12℃可能是薄皮甜瓜果实中糖代谢发生改变的临界低温。以上表明,夜间9℃胁迫条件下,薄皮甜瓜果实中蔗糖代谢、水苏糖代谢及淀粉代谢能力的下降共同影响果实的膨大生长和品质。

关键词: 薄皮甜瓜, 夜间低温, 糖, 糖代谢相关酶

Abstract:

【Objective】 The effects of different nigh low temperatures on carbohydrate metabolism in melon fruit were studied in this paper. 【Method】 Each carbohydrate content and sugar-metabolizing enzymes activities in fruits grew under normal and night low temperature circumstance simulated by climate room were analyzed. 【Result】 Effects of different night low temperatures on carbohydrate contents in melon fruit were different to some degrees. Fructose and glucose decreased after treatment of 9℃ in night for 3 d while significantly decreased sucrose content occurred in middle and later period, yet galactinol content had no change. Meanwhile, total carbohydrate content was lower than the control with markedly significant difference after treatment of 9℃ in night for 3 d, and the difference was more obvious as the time prolonging. Otherwise, compared with the control, starch content increased obviously on 3-12 d after treatment of 9℃ in night, however, after treatment of 12℃ in night, starch content increased in evidence while each carbohydrate content was almost invariable. Meanwhile, the change of sugar-metabolizing enzymes activities in fruit indicated that acid invertase (AI), netutral invertase (NI) and ?-galactosidase activities enhanced, but sucrose synthase (SS) activities and sucrose phosphate synthase (SPS) activities declined with very significant difference after treatment of 9℃ in night. What was more, the effects of 12℃ in night on sugar-metabolizing enzymes activities were small, with no significant difference. 【Conclusion】 Sugar accumulation and sugar-metabolizing enzymes activities were affected seriously after treatment of 9℃ in night, but 12℃ in night did not altered the carbohydrate metabolism, which was the critical low temperature to restrict sugar accumulation. These results suggested that the decreased metabolism including sucrose, stachyose and starch may affect melon fruit expansion and quality after treatment of 9℃ in night.

Key words: melon, night low temperature, carbohydrate, sugar-metabolizing enzymes