谷氨酰胺,天冬胺酰胺,肉鸡,脂肪性状,生脂基因," /> 谷氨酰胺,天冬胺酰胺,肉鸡,脂肪性状,生脂基因,"/> glutamine,aspartate,broiler,fatty traits,adipogenesis genes,"/> <font face="Verdana">谷氨酰胺和天冬胺酰胺对肉鸡部分脂肪性状及生脂基因mRNA表达水平的影响</font>

中国农业科学 ›› 2009, Vol. 42 ›› Issue (7): 2513-2522 .doi: 10.3864/j.issn.0578-1752.2009.07.032

• 畜牧·资源昆虫 • 上一篇    下一篇

谷氨酰胺和天冬胺酰胺对肉鸡部分脂肪性状及生脂基因mRNA表达水平的影响

孟德连,孙 超,姚军虎,杨公社,方 骏   

  1. (西北农林科技大学动物科技学院)
  • 收稿日期:2008-10-15 修回日期:2008-12-10 出版日期:2009-07-10 发布日期:2009-07-10
  • 通讯作者: 杨公社

Effect of Aspartate and Glutamine on a Part of the Fatty Traits and the Levels of Adipogenesis Genes mRNA Expression of Broiler#br#

MENG De-lian, SUN Chao, YAO Jun-hu, YANG Gong-she, FANG Jun#br#   

  1. (西北农林科技大学动物科技学院)
  • Received:2008-10-15 Revised:2008-12-10 Online:2009-07-10 Published:2009-07-10
  • Contact: YANG Gong-she

摘要:

【目的】选用320只1日龄AA肉公鸡,研究谷胺酰胺和天冬胺酰胺对肉鸡部分脂肪性状、血脂指标及相关生脂基因mRNA表达水平的影响,研究2种氨基酸对肉鸡脂代谢的影响机制。【方法】采用双因子完全随机设计,将试验鸡按体重随机分为4个处理组,分别在玉米-豆粕型基础饲粮中添加1%的谷氨酰胺和天冬胺酰胺,试验期为42 d。屠宰后测腹脂率,游标卡尺测皮下脂肪和肌间脂肪厚度,比色法测定血清甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白固醇(HDL-C),RT-PCR技术检测肝脏、腹脂组织中乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)、肉毒碱棕榈酰基转移酶Ⅰ(CPT-1)、脂蛋白酯酶(LPL)、肉毒碱棕榈酰基转移酶α(PPARα)mRNA水平的变化。【结果】饲粮中混合添加谷氨酰胺和天冬胺酰胺可显著提高肉鸡增重(P<0.05),对料肉比无显著影响(P>0.05),显著提高肌间脂肪宽和皮下脂肪厚(P<0.05),对腹脂率和全净膛率无显著影响(P>0.05);谷氨酰胺组和天冬胺酰胺组可显著降低腹脂率(P<0.05),显著增加皮下脂肪厚和肌间脂肪宽(P<0.05);添加谷氨酰胺和天冬胺酰胺后可显著降低血液TG含量(P<0.05),提高血液TC含量和降低HDL-C水平,影响血脂代谢。3个试验组肝脏中ACC、FAS、CPT-1、PPARα基因转录表达显著降低(P<0.05);谷氨酰胺组LPL 基因表达显著增加(P<0.05)。3个试验组脂肪组织PPARα转录表达显著增加(P<0.05),谷氨酰胺组和天冬胺酰胺组脂肪组织ACC转录表达显著降低(P<0.05),混合组脂肪组织FAS、LPL基因转录显著降低(P<0.05),而CPT-1转录则表达显著增加(P<0.05)。【结论】饲粮中添加谷胺酰胺和天冬胺酰胺可提高肉鸡生产性能,在一定程度上减少腹脂沉积,改善胴体性状。

关键词: 谷氨酰胺')">谷氨酰胺, 天冬胺酰胺, 肉鸡, 脂肪性状, 生脂基因

Abstract:

【Objective】 An experiment was conducted using a total of 320 one day old AA male broiler chickens to investigate the effect of dietary supplemented with glutamine and aspartic on fatty traits, blood fat levels, adipogenesis genes mRNA level in liver and coelo-grease and the possible mechanism of their function to lipid metabolism of broiler. 【Method】 The broilers were randomly tested by one of four treatments in a completely randomized design of treatments. They were fed by basic corn-soybean diet and basic diet supplemented with 1% glutamine and 1% aspartic, respectively, for a duration of 42 days. The rate of abdominal fat, the breadth of subcutaneous fat and the thickness of intermuscular fat was measured by vernier caliper. The levels of serum TG, TC, HDL-C were determined by ultraviolet spectrophotometer and the adipogenesis genes (ACC, FAS, CPT-1, LPL, PPARα) mRNA levels in liver and abdominal fat tissue were detected by RT-PCR technology after slaughtered. 【Result】 The addition of a mixed diet with aspartic acid and glutamine significantly improved the broiler weight gain (P<0.05) but not for feed conversion rate(P>0.05), significantly increased the width of intermuscular fat and subcutaneous fat thickness (P<0.05), but not for the percentage of abdominal fat and percentage of dressing (P>0.05). Supplemented with glutamine and the aspartic solo significantly reduced the rate of abdominal fat compared to control group (P<0.05), the two substances significantly increased in subcutaneous fat thickness and the width of intermuscular fat respectively (P<0.05). The addition of glutamine and aspartic had impact on lipid metabolism by significantly reducting the level of TG and HDL-C in blood (P<0.05), they also raised the content of TC level significantly at the same time (P<0.05). ACC, FAS, CPT-1, PPARα mRNA level of the three trial groups was significantly lower than the control group (P<0.05); the glutamine group LPL mRNA level increased significantly in liver (P<0.05). For adipose tissue, compared to the control group, the transcription expression of PPARα significantly increased in all three trial groups (P<0.05), glutamine group ACC mRNA level significantly decreased (P<0.05), FAS, LPL gene transcription level significantly reduced in the mixed group lower (P<0.05) but CPT-1 mRNA level significantly increased (P<0.05). The others did not change significantly (P>0.05). 【Conclusions】 The results indicate that the addition of glutamine and aspartate in dietary can raise growth performance of broiler, reduce abdominal fat deposition and improve the carcass fatty traits to a certain extent.

Key words: glutamine')">glutamine, aspartate, broiler, fatty traits, adipogenesis genes