关键词: 朗德鹅')">朗德鹅, 填饲, 产肝性能, 细胞形态, 脂肪酸组分, 基因表达

Abstract: 【Objective】 An experiment was conducted to study the candidate gene and early breeding projects of the fattty liver of Landes goose. 【Method】 Histological examination and analysis of fatty acids of the fatty liver were done using H & E stain and gas chromatography, respectively. The mRNA expression level of lipogenic genes was detected by real-time PCR. CT was used to investigate the fat deposition of living Landes goose. 【Result】 The result shown that compared to control group, overfeeding increased the liver weight, liver index, serum ALT, TG, Che, HDL levels and changed the positive ratio of liver and spleen to negative. Swelling of hepatocyte were observed within the cytoplasm of liver cells in goose treated with overfeeding compared to control. The values for the SFA and PUFA were significantly higher in control group than in overfeeding group. Higher values (P＜0.05) for MUFA and UFA in liver were found in goose in overfeeding group. Compared to the control group, the mRNA expression level of C/EBPα、ACCα increased in overfeeding group (P＜0.05). The positive correlation between the transcriptional level of ACCα and serum TG was observed (P＜0.01). 【Conclusion】 overfeeding can make the hepatocyte swelled and change the composition of fatty acids. The mRNA expression level of lipogenic genes increased, such as C/EBPα, ACCα. CT can be used to investigate the fat deposition of living Landes goose.

Key words: Lande goose')">Lande goose, overfeeding, liver performance, cell histology, fatty acid profile, expression of genes