关键词: 猪, 肝素结合性表皮生长因子, 多态性, 产仔数

Abstract:

【Objective】 The pig HB-EGF gene was used to study the polymorphism, and the association between the polymorphism and litter size was analyzed in order to find the molecular marker being used in marker-assisted selection(MAS) to accelerate the swine breeding progress. 【Method】 The polymorphism of HB-EGF gene was studied by PCR-SSCP, which the association between and litter size was studied by the least square mean, and the variance components at the two polymorphisms were also analyzed. 【Result】 There were two polymorphisms occurring in exon5 (primer E5) and exon6 (primer E7) respectively, each one with three genotypes (AA, BB and AB). At E5, in Large White, BB genotype had significantly higher (P＜0.05) TNB and NBA than AA genotype in third to eighth parity, and in all parities, BB genotype had significantly higher (P＜0.05) NBA than AA genotype, while had greatly significantly higher TNB than AA (P＜0.01), the heredity of TNB and NBA was mostly influenced by the genetic additive effect; In Landrace, there was no significant difference on TNB and NBA in the four phases. At E7, in Large White, AA genotype had significantly higher (P＜0.05) TNB than BB genotype in third to eighth parity and all parities, the heredity of TNB was mostly influenced by the genetic additive effect; in Landrace, AB genotype had significantly higher (P＜0.05) NBA than BB genotype in third to eighth parity and all parities. 【Conclusion】 In Large White, the BB genotype at E5 was favorable marker genotype for TNB and NBA, and the BB genotype at E7 was favorable marker genotype for TNB.

Key words: pig, heparin-binding EGF-like growth factor (HB-EGF), polymorphism, litter size