中国农业科学 ›› 2008, Vol. 41 ›› Issue (11): 3482-3488 .doi: 10.3864/j.issn.0578-1752.2008.11.007

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国干面条品质性状的QTL分析

赵京岚,陈民生,马艳明,李瑞军,任勇攀,孙庆泉,李斯深

  

  1. 山东农业大学农学院/国家作物生物学重点实验室

  • 收稿日期:2007-09-24 修回日期:2007-11-27 出版日期:2008-11-10 发布日期:2008-11-10
  • 通讯作者: 孙庆泉

  1. 山东农业大学农学院/国家作物生物学重点实验室

  • Received:2007-09-24 Revised:2007-11-27 Online:2008-11-10 Published:2008-11-10

摘要:

【目的】分析影响面条品质性状的QTL,了解面条品质的遗传控制。【方法】利用RIL群体和QTL分析软件进行QTL分析。面条感官品质按商业部行业标准(SB/T10137-93)测定,面条质构特性采用英国TA.XTplus型质构仪测定。【结果】检测到8个面条品质和质构特性的10个加性QTL,分别位于1A、1D、3D、4A和6D等5条染色体,单个QTL贡献率变化范围为4.07%~75.67%。在1D染色体Glu-D1附近检测到一个QTL簇,包括适口性、粘性和光滑性等3个性状各1个QTL,贡献率较高,增加效应均来自川35050,QTL间是正相关关系。在4A染色体上Xwmc420-Xswes620-Xswes615之间存在粘性和总分的QTL,贡献率较高,其增加效应来自亲本山农483,是正相关关系。在4A染色体Xswes624c-Xissr25b间食味QTL(QStas.sdau-4A.1)贡献率高达75.67%,是一个主效基因位点,其增加效应来自亲本山农483。【结论】检测到8个影响面条品质和质构特性的10个加性QTL,分析了其在染色体上的位置和效应。

关键词: 小麦, 面条, 品质性质, 质构特性, QTL, RIL

Abstract: 【Objective】the objective of this study were to map the QTLs for quality traits of Chinese dry noodle and obtain the genetic control of noodle.【Method】The QTLs for the noodle traits were mapped based on a population of recombinant inbred lines (RIL) derived from the cross between two Chinese winter wheat varieties, Chuang 35050 and Shannong 483. The QTLMapper Version 1.0 was employed to analyses the QTLs. Noodle sensory qualities were tested according to the standard of Ministry of Commerce of P. R. China (SB/T10137-93). The textural properties were detected using a Texture Analyser (QTS-25).【Result】We have obtained 10 additive QTL traits of 8 noodle quality traits with a single QTL explained 7.99-40.52% of phenotypic variations on chromosomes 1A,1D, 3D, 4A, and 6D. A cluster of 3 QTLs for noodle palate, elasticity and smoothness was found near the Glu-D1 locus on 1D chromosome with higher contributions. The increasing effect come from subunit 5+10 of Chuan 350505, and the relationship between the QTLs were positive. On chromosomes 4A, co-location QTLs for stickiness and total score were detect at the region of Xwmc420-Xswes620- Xswes615. Their contributions were higher and the increasing effects come from Shannong 483. A taste QTL——QStas.sdau-4A.1 was obtained at Xswes624c-Xissr25b on chromosome 4A with the highst contribution of 75.63%. This QTL was a mainly gene and the increasing effects come from Shannong 483. 【Conclusion】We have obtained the chromosome locations and effects of 10 additive QTLs of 8 noodle quality traits.