中国农业科学 ›› 2008, Vol. 41 ›› Issue (3): 823-827 .doi: 10.3864/j.issn.0578-1752.2008.03.026

• 畜牧·资源昆虫 • 上一篇    下一篇

用杂种克隆板将猪GAS6基因物理定位于SSC11

马海明,柳小春,施启顺   

  1. 湖南农业大学动物科技学院
  • 收稿日期:2006-11-20 修回日期:2007-03-12 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 柳小春

Mapping the GAS6 Gene of Porcine to SSC11q11-17 with SCHP

  

  1. 湖南农业大学动物科技学院
  • Received:2006-11-20 Revised:2007-03-12 Online:2008-03-10 Published:2008-03-10

摘要: 【目的】确定GAS6(growth arrested-special 6)基因在染色体的物理位置,为该基因的克隆及功能研究打下基础。【方法】运用比较基因学的方法,根据人的该基因序列设计引物,从大围子猪基因组分离猪该基因的内含子9的片段,通过扩增体细胞杂种克隆板上27个样品和辐射克隆板上118个样品。【结果】分离了猪该基因包括内含子9的片段(GenBank收录号为AY880668),首次将GAS6基因物理定位于猪SSC11 q11-17,与微卫星SW1452标记紧密连锁,LOD值为16.88,存留率为22%,在放射杂交图谱上的图距为56 cR。【结论】GAS6基因区间定位结果与精细定位结果相一致,也与比较定位结果相一致,并进一步验证了猪11号染色体(SSC11)和人大部分13号染色体(HSA13)存在同源性。

关键词: 猪, 放射杂交板, 物理定位, GAS6基因

Abstract: Abstract: ObjectiveGAS6growth arrested-special 6is associated with adhesion, identification, survival, proliferation, differentiation, and growth of cell in human. However, little is known about gene of GAS6 of porcine. In order to map the position of the gene of GAS6 in chromosome in pigs and to found the basis of cloning this gene and studying the function of this gene, Methodwe used the Pig別rodent somatic cell hybrid panel(SCHP) and INRA-Minnesota porcine radiation hybrid panel(IMpRH). Results the isolated fragments GAS6 gene of swine was deposited into GenBank and accession number was AY880668. The results showed that the gene of GAS6 was located in the SSC11q11-17. The GAS6 gene were linkaged closely to the microsatellite SW1452 anchored on SSC11 with the rate of retension of 0.22, LOD score threshold were 16.88 and the distance in map of the radiation hybrid (RH) was 56 cR. ConclusionThat the position of GAS6 was on the SSC11q11-17 conformed to the conclusions which was drawn by means of IMpRH because the SSC11 is homologous with most of HSA11.