Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (3): 512-520.doi: 10.3864/j.issn.0578-1752.2019.03.011

• HORTICULTURE • Previous Articles     Next Articles

Using Chlorine Dioxide Treatment to Promote Wound Healing of Postharvest Muskmelon Fruit

ZHENG XiaoYuan,WANG TiaoLan,ZHANG JingRong,JIANG Hong,WANG Bin,BI Yang()   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070
  • Received:2018-07-18 Accepted:2018-09-13 Online:2019-02-01 Published:2019-02-14

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Abstract:

【Objective】 The aims of this study were to investigate effect of chlorine dioxide (ClO2) treatment on the wound healing of harvested muskmelons and its mechanism, providing methods and theoretical basis for wound healing of postharvest muskmelon fruit.【Method】 The muskmelon fruit ‘cv. Manao’ was used as material. After artificially wounded, fruits were dipped with ClO2 at 25 mg?L -1 for 10 min, and the treated fruit and control were wound healed at ambient temperature in dark. The weight loss of fruit and the disease index of Trichotechium roseum inoculated fruit were measured during healing. The accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at the wounded sites of fruit were observed by Toluidin blue O-neutral red staining and phloroglucinol-HCl staining method, and the amount of accumulation of the three compositions were measured by IS Capture image software. Moreover, the color values of wounded surface were measured. The enzyme activities of phenylpropanoid metabolism and changes of peroxidase and polyphenol oxidase enzyme activities were analyzed during the fruit wound healing stage. 【Result】 The weight loss of wounded fruit and the disease index of inoculated fruit were significantly reduced by ClO2 treatment. The weight loss of treated fruit was 10.3% lower than that of control after 7 days of healing. Wounded fruit were inoculated by Trichotechium roseum at different wound healing periods. After one week cultivated, the disease index of the treated fruit was significantly lower than that of control, which was 56.9% lower at 7 days of healing. The ClO2 treatment significantly promoted the accumulation of suberin poly phenolic, suberin poly aliphatic and lignin. The treated fruit were significantly higher than that of control at the mid and late stage of healing. After 7 days of healing, the thickness of suberin poly phenolic cell layers, suberin poly aliphatic and lignin cell layers of treated fruit was 25.3%, 77.7% and 35.5% higher than that of control, respectively. Compared with the control, the L* value of wounded surface in the treated fruit was significantly lower and b* value was significantly higher during healing. The L* value of the treated fruit was 6.1% lower than that of control after 5 days of healing. And the b* value of the treated fruit was 17.8% higher than that of control after 3 days of healing. The ClO2 treatments increased enzymes activities of phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase at wounded sites of fruit, which were 34.3%, 80.5% and 15.7% higher than that of control after 7 days of healing, respectively. Meanwhile, the treatment also improved the accumulation of total phenols, flavonoids and lignin at wounded sites, which were 14.7%, 16.8%, and 15.6% higher than that of control after 7 days of healing, respectively.【Conclusion】 ClO2 treatment effectively promoted wound healing of harvested muskmelons by eliciting the phenylpropanoid metabolism. In addition, it also increased the enzymes activities of peroxidase and polyphenol oxidase, and promoted accumulation of suberin and lignin at wounded sites.

Key words: chlorine dioxide, muskmelons, postharvest, wound healing

Fig. 1

Effect of ClO2 treatment on weight loss (A) and disease index (B) of muskmelon fruit during wound healing * indicate significant differences (P<0.05). The same as below"

Fig. 2

Effect of ClO2 treatment on the accumulation of SPP (A), SPA (B) and lignin (C) of muskmelon wounded sites during wound-healing (×100)"

Fig. 3

Effects of ClO2 treatment on thickness of SPP (A), SPA (B) and lignified cell layers (C) of muskmelon wounded sites during wound-healing"

Fig. 4

Effects of ClO2 treatment on L* (A) and b* (B) of muskmelon wounded site during wound healing"

Fig. 5

Effects of ClO2 treatment on the activity of PAL(A), POD(B) and PPO(C), and the content of total phenolic (D), flavonoids (E) and lignin (F) in muskmelon wounded sites during wound-healing"

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