Physiological response of four wolfberry (Lycium Linn.) species under drought stress
ZHAO Jian-hua1, LI Hao-xia2, ZHANG Cun-zhi3, AN Wei1, YIN Yue1, WANG Ya-jun1, CAO You-long1
1 National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, P.R.China 2 Desertification Control Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, P.R.China 3 Ningxia Professional Technology College, Yinchuan 750021, P.R.China
Abstract We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species (Lycium chinense Mill. var. potaninii (Pojark.) A. M. Lu, Lycium chinense Mill., Lycium barbarum L., and Lycium yunnanense Kuang & A. M. Lu) under four water supply regimes. In all four species, drought affected seedlings in terms of chlorophyll content, net photosynthesis rate (Pn), transpiration rate (E), and lipid peroxidation. Drought also increased some antioxidant enzyme activities, such as peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Significant changes in dry biomass partitioning also occurred in response to water stress. In particular, dry biomass of leaves and fruits decreased significantly. L. chinense Mill. and L. barbarum L. possessed greater drought tolerance and exhibited superior antioxidant processing ability and other related physiological traits compared to the other two species. L. chinense Mill. was the most tolerant to all levels of drought. In contrast, L. yunnanense Kuang & A. M. Lu was more affected by water supply and had the lowest resistance to drought stress. These findings would provide some important information regarding genetic resources for future forest tree improvement in relation to drought tolerance.
This study was financially supported by the National Natural Science Foundation of China (31360191, 31660220), the Natural Science Foundation of Ningxia Hui Autonomous Region, China (NZ16121), and the Self-option and Foundation of Ningxia Academy of Agriculture and Forestry Sciences, China (YES-16-0402, NKYZ-16-0402).
ZHAO Jian-hua, LI Hao-xia, ZHANG Cun-zhi, AN Wei, YIN Yue, WANG Ya-jun, CAO You-long. Physiological response of four wolfberry (Lycium Linn.) species under drought stress[J]. Journal of Integrative Agriculture,
2018, 17(03): 603-612.
Anjum S A, Xie X, Wang L C, Saleem M F, Man C, Lei W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6, 2026-2032.
Arnon D I. 1949. Copper enzymes in isolated chloroplasts: Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24, 1-15.
Aroca R, Irigoyen J J, Sánchez-díaz M. 2003. Drought enhances maize chilling tolerance. II. Photosynthetic traits and protective mechanisms against oxidative stress. Physiologia Plantarum, 117, 540-549.
Ashraf M, Iram A. 2005. Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora, 200, 535-546.
Aacute;vila C, Guardiola J L, Nebauer S G. 2012. Response of the photosynthetic apparatus to a flowering-inductive period by water stress in citrus. Trees, 26, 833-840.
Babita M, Maheswari M, Rao L M, Shanker A K, Rao D G. 2010. Osmotic adjustment, drought tolerance and yield in castor (Ricinus communis L.) hybrids. Environmental and Experimental Botany, 69, 243-249.
Bates L S, Waldren R P, Teare I D. 1973. Rapid determination of free proline for water-stress studies. Plant & Soil, 39, 205-207.
Bhatt D, Negi M, Sharma P, Saxena S C, Dobriyal A K, Arora S. 2011. Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution. Physiology & Molecular Biology of Plants, 17, 347-353.
Bilger W, Björkman O. 1990. Role of the xanthophyll cycle in photo-protection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynthesis Research, 25, 173-185.
Bruna D S, Bruno M R, Laurício E, Mauro G S. 2010. Ecophysiology parameters of four Brazilian Atlantic Forest species under shade and drought stress. Acta Physiologiae Plant, 32, 729-737.
Chang R C, So K F. 2008. Use of anti-aging herbal medicine, Lycium barbarum, against aging-associated diseases. What do we know so far? Cellular & Molecular Neurobiology, 28, 643-652.
Chaves M M. 1991. Effects of water deficits on carbon assimilation. Journal of Experimental Botany, 42, 1-16.
Chaves M M, Oliveira M M. 2005. Mechanisms underlying plant resilience to water deficits: Prospects for water-saving agriculture. Journal of Experimental Botany, 55, 2365-2384.
Chen J Y, Zhang Z T, Li Y N. 2004. Effects of NaCl stress on betaine, chloroplast pigment of leaves chlorophyll fluorescence and of Lycium barbarum L. Agricultural Research in the Arid Areas, 22, 109-114. (in Chinese)
Chen M T, Zhao Z. 2011. Effects of drought on root characteristics and mass allocation in each part of seedlings of four tree species. Journal of Beijing Forestry University, 33, 16-22. (in Chinese)
CCP (Committee of Chinese Pharmacopoeia). 2010. Chinese Pharmacopoeia. Committee of Chinese Pharmacopoeia, Beijing. (in Chinese)
Deng B, Du W, Liu C, Sun W, Tian S, Dong H. 2012. Antioxidant response to drought, cold and nutrient stress in two ploidy levels of tobacco plants: Low resource requirement confers polytolerance in polyploids? Plant Growth Regulation, 66, 37-47.
Diego N D, Pérezalfocea F, Cantero E, Lacuesta M, Moncaleán P. 2012. Physiological response to drought in radiata pine: Phytohormone implication at leaf level. Tree Physiology, 32, 435-449.
Gao D, Gao Q, Xu H Y, Ma F, Zhao C M, Liu J Q. 2009. Physiological responses to gradual drought stress in the diploid hybrid Pinus densata and its two parental species. Trees, 23, 717-728.
Genty B, Briantais J M, Baker N R. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 990, 87-92.
Hodges D M, DeLong J M, Forney C F, Prange R K. 1999. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207, 604-611.
Horváth E, Pál M, Szalai G, Páldi E, Janda T. 2007. Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short term drought and freezing stress on wheat plants. Biologia Plantarum, 51, 480-487.
Inskeep W P, Bloom P R. 1985. Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiology, 77, 483-485.
Li H C, Qiu Z J. 2003. A review of studies of drought resistance in tree species and drought resistant forestation technology. World Forestry Research, 16, 17-22.
Michel H, Florence T. 1999. Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress. Australian Journal of Plant Physiology, 26, 569-578.
Reddy A R, Chaitanya K V, Vivekanandan M. 2004. Drought-induced response of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161, 1189-1202.
Ribas-Carbo M, Taylor N L, Giles L, Busquets S, Finnegan P M, Day D A, Lambers H, Medrano H, Berry J A, Flexas J. 2005. Effects of water stress on respiration in soybean leaves. Plant Physiology, 139, 466-473.
Schreiber U, Schliwa U, Bilger W. 1986. Continuous recording of pho-tochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynthesis Research, 10, 51-62.
Toorchi M, Shashidhar H E, Hittalmani S, Gireesha T M. 2002. Rice root morphology under contrasting moisture regimes and contribution of molecular marker heterozygosity. Euphytica, 126, 251-257.
Wallin G, Karlsson P E, Selldn G, Ottosson S, Medin E L, Pleijel H, Skarby L. 2002. Impact of four years exposure to different levels of ozone, phosphorus and drought on chlorophyll, mineral nutrients, and stem volume of Noway spruce, Picea abies. Physiologia Plantarum, 11, 192-206.
Wang Y, Ma F, Li M, Liang D, Zou J. 2011. Physiological responses of kiwifruit plants to exogenous ABA under drought conditions. Plant Growth Regulation, 64, 63-74.
Xiao Y A. 2001. The physiology responses and adjective adaptability of water stress on Cleme spinosa L. seedlings. Journal of Wuhan Botanical Research, 19, 524-528. (in Chinese)
Xie C, Xu L Z, Li X M, Zhao B H, Yang S L. 2001. Studies on chemical constituents in fruit of Lycium barbarum L. China Journal of Chinese Materia Medica, 26, 323-324. (in Chinese)
Xu Y H, Xu Y, An W T. 2000. The progress in studies on anti-tumor pharmacodynamics of Lycium barbarum Medicine and Materia Medica Research, 11, 946-947. (in Chinese)
Zobel R W. 2003. Sensitivity analysis of computer based diameter measurement from digital images. Crop Science, 43, 583-591.