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| Identification of the DEAD-box RNA helicase family members in grapevine reveals that VviDEADRH25a confers tolerance to drought stress |
| YANG Sheng-di1, 2, GUO Da-long1, 2, PEI Mao-song1, 2, WEI Tong-lu1, 2, LIU Hai-nan1, 2, BIAN Lu1, 2, YU Ke-ke1, 2, ZHANG Guo-hai1, 2, YU Yi-he1, 2 |
1 College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, P.R.China
2 Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, Luoyang 471023, P.R.China |
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摘要
DEAD-box是RNA解旋酶家族中最大的亚家族之一，在植物的生长发育及其逆境胁迫中发挥着关键作用。此前有研究表明了DEAD-box基因在拟南芥和番茄、水稻等作物物种干旱胁迫响应中的潜力，但有关葡萄DEAD-box基因的功能尚不清楚。本研究在葡萄中共鉴定出40个DEAD-box基因，并对其蛋白序列特征、基因结构、染色体位置、顺式作用元件进行了分析，并比较了VviDEADRHs在干旱胁迫下的表达模式，筛选出9个可能在葡萄干旱胁迫过程中发挥关键作用VviDEADRHs (VviDEADRH10c，-13，-22，-25a，-25b，-33，-34，-36，-39)。结合qRT-PCR结果，选择VviDEADRH25a进行功能验证。在拟南芥中过表达VviDEADRH25a促使植株对干旱胁迫更加敏感，电解质渗透率和丙二醛含量均显著增加，叶绿素含量、超氧化物歧化酶 (SOD)、过氧化物酶 (POD)、过氧化氢酶 (CAT)和抗坏血酸过氧化物酶 (APX)酶活性显著降低。此外，VviDEADRH25a过表达植株中与干旱胁迫相关的几个标记基因 (AtCOR15a、AtRD29a、AtERD15、AtP5CS1) 的表达水平下调。综上所述，本研究为葡萄DEAD-box RNA解旋酶基因的结构、进化提供了理论基础，为提高葡萄干旱耐受性研究提供了新的见解。
Abstract Grapevine growing areas are increasingly affected by drought, which has greatly limited global wine production and quality. DEAD-box is one of the largest subfamilies of the RNA helicase family, and its members play key roles in the growth and development of plants and their stress responses. Previous studies have shown the potential of DEAD-box genes in the drought stress responses of Arabidopsis and tomato, rice, and other crop species. However, information about DEAD-box genes in grapevine remains limited. In this report, a total of 40 DEAD-box genes were identified in grapevine and their protein sequence characteristics and gene structures were analyzed. By comparing the expression profiles of VviDEADRHs in response to drought stress in different grapevine varieties, nine candidate genes (VviDEADRH10c, -13, -22, -25a, -25b, -33, -34, -36, and -39) were screened based on expression profiling data. Combined with qRT-PCR results, VviDEADRH25a was selected for functional verification. Heterologous overexpression of VviDEADRH25a in Arabidopsis showed the transgenic plants were more sensitive to drought stress than the control. Both electrolyte permeability and malondialdehyde content were significantly increased in transgenic plants, whereas the chlorophyll content and superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) enzyme activities were significantly decreased. Furthermore, VviDEADRH25a-overexpressing plants showed down-regulated expression levels of several drought stress-related marker genes, namely AtCOR15a, AtRD29A, AtERD15, and AtP5CS1, which indicated that they participated in the drought stress response. In summary, this study provides new insights into the structure, evolution, and participation of DEAD-box RNA helicase genes in the response to drought stress in grapevines.
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Received: 16 November 2020
Accepted: 25 November 2021
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| Fund: This study was financially supported by grants from the National Natural Science Foundation of China (32072517), the National Key Research and Development Program of China (2018YFD1000105), the Program for Science & Technology Innovation Talents in Universities of Henan Province, China (21HASTIT035), the Program for Innovative Research Team (in Science and Technology) in University of Henan Province, China (21IRTSTHN021), and the Science and Technology Planning Project of Luoyang City, China (2101102A). |
| About author: YANG Sheng-di, E-mail: yangshengdi2050@163.com; Correspondence YU Yi-he, E-mail: yuyihe@haust.edu.cn |
Cite this article:
YANG Sheng-di, GUO Da-long, PEI Mao-song, WEI Tong-lu, LIU Hai-nan, BIAN Lu, YU Ke-ke, ZHANG Guo-hai, YU Yi-he.
2022.
Identification of the DEAD-box RNA helicase family members in grapevine reveals that VviDEADRH25a confers tolerance to drought stress. Journal of Integrative Agriculture, 21(5): 1357-1374.
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