Characterization of salt tolerance and Fusarium wilt resistance of a sweetpotato mutant
ZHANG Huan, ZHANG Qian, WANG Yan-nan, LI Yan, ZHAI Hong, LIU Qing-chang, HE Shao-zhen
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China
Abstract The variant LM1 was previously obtained using embryogenic cell suspension cultures of sweetpotato variety Lizixiang by gamma-ray induced mutation, and then its characteristics were stably inherited through six clonal generations, thus this mutant was named LM1. In this study, systematic characterization of salt tolerance and Fusarium wilt resistance were performed between Lizixiang and mutant LM1. LM1 exhibited significantly higher salt tolerance compared to Lizixiang. The content of proline and activities of superoxide dismutase (SOD) and photosynthesis were significantly increased, while malonaldehyde (MDA) and H2O2 contents were significantly decreased compared to that of Lizixiang under salt stress. The inoculation test with Fusarium wilt showed that its Fusarium wilt resistance was also improved. The lignin, total phenolic, jasmonic acid (JA) contents and SOD activity were significantly higher, while H2O2 content was significantly lower in LM1 than that in Lizixiang. The expression level of salt stress-responsive and disease resistance-related genes was significantly higher in LM1 than that in Lizixiang under salt and Fusarium wilt stresses, respectively. This result provides a novel and valuable material for improving the salt tolerance and Fusarium wilt resistance of sweetpotato.
This research was supported by the National Natural Science Foundation of China (31371680), the Beijing Food Crops Innovation Consortium Program, China (BAIC09-2016) and the earmarked fund for the China Agriculture Research System (CARS-11).
Corresponding Authors: Correspondence HE Shao-zhen, Tel/Fax: +86-10-62733710, E-mail: email@example.com
Cite this article:
ZHANG Huan, ZHANG Qian, WANG Yan-nan, LI Yan, ZHAI Hong, LIU Qing-chang, HE Shao-zhen. Characterization of salt tolerance and Fusarium wilt resistance of a sweetpotato mutant[J]. Journal of Integrative Agriculture,
2017, 16(09): 1946-1955.
Ajithkumar I P, Panneerselvam R. 2014. ROS scavenging system, osmotic maintenance, pigment and growth status of Panicum sumatrense Roth. under drought stress. Cell Biochemistry and Biophysics, 68, 587-595.
Apel K, Hirt H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 728-749.
Bandyopadhyay U, Das D, Banerjee R K. 1999. Reactive oxygen species: Oxidative damage and pathogenesis. Current Science, 77, 658-666.
Bending G D, Read D J. 1997. Lignin and soluble phenolic degradation by ectomycorrhizal and ericoid mycorrhizal fungi. Mycological Research, 101, 1348-1354.
Chai G H, Qi G, Cao Y P, Wang Z G, Yu L, Tang X F, Yu Y C, Wang D, Kong Y Z, Zhou G K. 2014. Poplar PdC3H17 and PdC3H18 are direct targets of PdMYB3 and PdMYB21, and positively regulate secondary wall formation in Arabidopsis and poplar. New Phytologist, 203, 520-534.
Deng X M, Hu W, Wei S Y, Zhou S Y, Zhang F, Han J P, Chen L H, Li Y, Feng J L, Fang B, Luo Q C, Li S S, Liu Y Y, Yang G X, He G Y. 2013. TaCIPK29, a CBL-interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco. PLOS ONE, 8, e69881.
Hammerschmidt R, Ku? J. 1982. Lignification as a mechanism for induced systemic resistance in cucumber. Physiological Plant Pathology, 20, 61-71.
Hare P D, Cress W A. 1997. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulation, 21, 79-102.
He S Z, Han Y F, Wang Y P, Zhai H, Liu Q C. 2009. In vitro selection and identification of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl. Plant Cell, Tissue and Organ Culture, 96, 69-74.
Heller J, Tudzynski P. 2011. Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease. Annual Review of Phytopathology, 49, 369-390.
Hückelhoven R. 2007. Cell wall-associated mechanisms of disease resistance and susceptibility. Annual Review of Phytopathology, 45, 101-127.
Kim Y H, Lim S, Han S H, Lee J J, Nam K J, Jeong J C, Lee H S, Kwak S S. 2015. Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweetpotato plants. Comptes Rendus Biologies, 338, 307-313.
Lattanzio V, Lattanzio V M, Cardinali A. 2006. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Advances in Research, 661, 23-67.
Liu D G, He S Z, Zhai H, Wang L J, Zhao Y, Wang B, Li R J, Liu Q C. 2014. Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato. Plant Cell, Tissue and Organ Culture, 117, 1-16.
Liu Q C, Zhai H, Wang Y, Zhang D. 2001. Efficient plant regeneration from embryogenic suspension cultures of sweetpotato. In Vitro Cellular & Developmental Biology-Plant, 37, 564-567.
Luan Y S, Zhang J, Gao X R, An L J. 2007. Mutation induced by ethylmethanesulphonate (EMS), in vitro screening for salt tolerance and plant regeneration of sweetpotato (Ipomoea batatas L.). Plant Cell, Tissue and Organ Culture, 88, 77-81.
Maggio A, Miyazaki S, Veronese P, Fujita T, Ibeas J I, Damsz B, Narasimhan M L, Hasegawa P M, Joly R J, Bressan R A. 2002. Does proline accumulation play an active role in stress-induced growth reduction? The Plant Journal, 31, 699-712.
Matta A. 1989. Induced resistance to Fusarium wilt diseases. In: Tjamos E C, Beckman C H, eds., Vascular Wilt Diseases of Plants. Springer, Berlin Heidelberg. pp. 175-196.
Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. 2010. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell & Environment, 33, 453-467.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and Bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497
Nicholson R L, Hammerschmidt R. 1992. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, 30, 369-389.
Ogawa K, Komada H. 1985. Biological control of Fusarium wilt of sweetpotato with cross-protection by prior inoculation with nonpathogenic Fusarium oxysporum. Japan Agricultural Research Quarterly, 19, 20-25.
Ronde J A D, Cress W A, Krüger G H J, Strasser R J, Staden J V. 2004. Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress. Journal of Plant Physiology, 161, 1211-1224.
Saradhi P P, Mohanty P. 1991. Proline enhances primary photochemical activities in isolated thylakoid membranes of Brassica juncea by arresting photoinhibitory damage. Biochemical and Biophysical Research Communications, 181, 1238-1244.
Saradhi P P, Mohanty P. 1997. Involvement of proline in protecting thylakoid membranes against free radical-induced photodamage. Journal of Photochemistry and Photobiology (B: Biology), 38, 253-257.
Shapiro R S, Robbins N, Cowen L E. 2011. Regulatory circuitry governing fungal development, drug resistance, and disease. Microbiology and Molecular Biology Reviews, 75, 213-267.
Syros T, Yupsanis T, Zafiriadis H, Economou A. 2004. Activity and isoforms of peroxidases, lignin and anatomy, during adventitious rooting in cuttings of Ebenus cretica L. Journal of Plant Physiology, 161, 69-77.
Takahashi S, Murata N. 2008. How do environmental stresses accelerate photoinhibition? Trends in Plant Science, 13, 178-182.
Thomma B P, Eggermont K, Penninckx I A, Mauch-Mani B, Vogelsang R, Cammue B P, Broekaert W F. 1998. Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proceedings of the National Academy of Sciences of the United States of America, 95, 15107-15111.
Thomma B P, Penninckx I A, Cammue B P, Broekaert W F. 2001. The complexity of disease signaling in Arabidopsis. Current Opinion in Immunology, 13, 63-68.
Turner J G, Ellis C, Devoto A. 2002. The jasmonate signal pathway. The Plant Cell, 14, 153-164.
Velioglu Y, Mazza G, Gao L, Oomah B. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46, 4113-4117.
Wang W B, Kim Y H, Lee H S, Kim K Y, Deng X P, Kwak S S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry, 47, 570-577.
Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Wang W. 2001. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology, 127, 315-323.
Yang R, Jarvis D E, Chen H, Beilstein M A, Grimwood J, Jenkins J, Shu S, Prochnik S, Xin M, Ma C. 2013. The reference genome of the halophytic plant Eutrema salsugineum. Frontiers in Plant Science, 4, 46.
Zhai H, Wang F B, Si Z Z, Huo J X, Xing L, An Y Y, He S Z, Liu Q C. 2015. A myo-inositol-1-phosphate synthase gene, IbMIPS1, enhances salt and drought tolerance and stem nematode resistance in transgenic sweetpotato. Plant Biotechnology Journal, 14, 592-602.