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Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2279-2289    DOI: 10.1016/S2095-3119(16)61399-0
Physiology·Biochemistry·Cultivation·Tillage Advanced Online Publication | Current Issue | Archive | Adv Search |
Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativa L.)
WANG Fa-wei1*, WANG Chao1*, SUN Yao1*, WANG Nan1, LI Xiao-wei1, DONG Yuan-yuan1, Yao Na1, Liu Xiu-ming1, CHEN Huan1, CHEN Xi-feng2, WANG Zhen-min2, LI Hai-yan1
1 College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China
2 Jilin Technology Innovation Center for Soybean Region, Jilin Agricultural University, Changchun 130118, P.R.China
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Abstract      The vacuolar proton pump ATPase (V-H+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H+-ATPase subunits B (ScVHA-B, GenBank: JF826506), C (ScVHA-C, GenBank: JF826507) and H (ScVHA-H, GenBank: JF826508) were isolated from the halophyte Suaeda corniculata. The transcript levels of ScVHA-B, ScVHA-C and ScVHA-H were increased by salt, drought and saline-alkali treatments. V-H+-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H+-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H+-ATPase subunits B, C and H (ScVHA-B, ScVHA-C and ScVHA-H) from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic linevacuolar proton pump, salt tolerance, saline-alkali tolerance, alfalfa
s carrying the B, C and H subunits had higher germination rates than the wild type (WT). More free proline, higher superoxide dismutase (SOD) activity and lower malondialdehyde (MDA) levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the ScVHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of ScVHA-B, ScVHA-C and ScVHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.
Keywords:  vacuolar proton pump        salt tolerance        saline-alkali tolerance        alfalfa  
Received: 08 December 2015   Accepted:

This research was supported by the National Natural Science Foundation of China (31271746, 31401403, 31501366, 31201237).

Corresponding Authors:  LI Hai-yan, Tel: +86-431-84532885, Fax: +86-431-84533427, E-mail:   
About author:  WANG Fa-wei, Mobile: +86-13596076186, E-mail:

Cite this article: 

WANG Fa-wei, WANG Chao, SUN Yao, WANG Nan, LI Xiao-wei, DONG Yuan-yuan, Yao Na, Liu Xiu-ming, CHEN Huan, CHEN Xi-feng, WANG Zhen-min, LI Hai-yan. 2016. Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativa L.). Journal of Integrative Agriculture, 15(10): 2279-2289.

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