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| Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs |
| ZHAO Shuan-ping1, 4, TANG Zhong-lin1, ZHOU Rong1, QU Chang-qing3, ZHENG Jian-wei2 and LI Kui1 |
1、Key Laboratory for Domestic Animal Genetic Resources and Breeding, Ministry of Agriculture/Institute of Animal Science, Chinese Academy
of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R.China
3、School of Life Science, Fuyang Teachers College, Fuyang 236041, P.R.China
4、Institute of Animal Science, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China |
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摘要 Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G>A) and g. 2992 (G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Duroc×(Landrace×Yorkshire)) pig breeds.
Abstract Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G>A) and g. 2992 (G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Duroc×(Landrace×Yorkshire)) pig breeds.
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Received: 05 December 2012
Accepted:
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| Fund: This work was supported by the National High Technology Research and Development Program of China (2011AA100302), the National Key Technology R&D Program of China (2011ZX08009-001), the National Natural Science Foundation of China (31171192), and the National Basic Research Program of China (2012CB124706). |
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Corresponding Authors:
ZHENG Jian-wei, E-mail: zjw2001309@hotmail.com
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| About author: ZHAO Shuan-ping, E-mail: zhaoshuanping@163.com |
Cite this article:
ZHAO Shuan-ping1, 4 , TANG Zhong-lin1, ZHOU Rong1, QU Chang-qing3, ZHENG Jian-wei2 and LI Kui1.
2014.
Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs. Journal of Integrative Agriculture, 13(5): 1051-1057.
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