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Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm |
HE Yu-xuan, LI Hong-yan, ZHANG Yong, HE Jian-hua, ZHANG Hong, ZHAO Xing-xu |
1.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R.China
2.Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R.China
3.Key Laboratory of Heavy Ion Radiation Medicine, Chinese Academy of Sciences, Lanzhou 730000, P.R.China
4.Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, P.R.China
5.University of Chinese Academy of Sciences, Beijing 100049, P.R.China
6.Lanzhou Agricultural Science and Technology Research Promotion Center, Lanzhou 730030, P.R.China |
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摘要 This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis (2-DE) analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry (LC-MS/MS). Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases (NCBI) indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha (TFAP-2α). The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.
Abstract This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis (2-DE) analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry (LC-MS/MS). Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases (NCBI) indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha (TFAP-2α). The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.
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Received: 26 March 2012
Accepted:
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Fund: This work was supported by the Key Scientific Technology Research Projects of Gansu Province, China (1102NKDA022), the Major Program of Ministry of Agriculture to Cultivate New Varieties of Genetically Modified Organisms of China (2008ZX08008-003), the National Basic Research Program of China, the National Natural Science Foundation of China (2010CB834202). |
Corresponding Authors:
Correspondence ZHAO Xing-xu, Tel: +86-931-7632482, E-mail: zhaoxx@gsau.
edu.cn; ZHANG Hong, Tel: +86-931-4969344, E-mail: zhangh@impcas.ac.cn
E-mail: zhangh@impcas.ac.cn
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About author: HE Yu-xuan, E-mail: heyuxuan1226@126.com; LI Hong-yan, E-mail: lihyedu@163.com |
Cite this article:
HE Yu-xuan, LI Hong-yan, ZHANG Yong, HE Jian-hua, ZHANG Hong, ZHAO Xing-xu.
2013.
Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm. Journal of Integrative Agriculture, 12(9): 1629-1637.
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