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Journal of Integrative Agriculture  2012, Vol. 12 Issue (10): 1580-1591    DOI: 10.1016/S1671-2927(00)8691
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Isolation and Functional Analysis of the bZIP Transcription Factor Gene TaABP1 from a ChineseWheat Landrace
CAO Xin-you, CHEN Ming*, XU Zhao-shi, CHEN Yao-feng, LI Lian-cheng, YU Yue-hua, LIU Yangna, MA You-zhi
1.National Key Facility for Crop Genetic Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.China
3.College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
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摘要  In plants, basic leucine zipper (bZIP) transcription factors play important roles in regulatory processes, including stress response, pathogenic defense and light response as well as organ and tissue differentiation. Chinese wheat landrace Pingyaoxiaobaimai (PYXBM), an original parent of drought tolerant wheat varieties grown in northern China, is significantly tolerant to abiotic stresses such as drought, cold and nutrient deficiencies. In order to isolate key stress-responsive genes and then improve stress tolerances of conventional varieties, a bZIP transcription factor gene was isolated from a cDNA library of drought-treated PYXBM using the in situ plaque hybridization method, and was designated as Triticum aestivum L. abscisic acid (ABA)-responsive element binding protein 1 (TaABP1). It encodes 372 amino acids, and contains three conserved domains (C1-C3) in the N terminal and a bZIP domain in the C terminal which is a typical protein structure for the group member of bZIP family. Transcriptional activation analysis showed that TaABP1 activated the expression of downstream reporter genes in yeast without ABA application. TaABP1 protein fused with green fluorescent protein (GFP) demonstrated that the localization of TaABP1 protein is in the nucleus. Expression pattern assays indicated that TaABP1 was strongly induced by ABA, high salt, low temperature and drought, and its expression was stronger in stems and leaves than in the roots of wheat. Furthermore, overexpression of TaABP1 in tobacco showed significant improvement of drought tolerance. Data suggested that TaABP1 may be a good candidate gene for improving stress tolerance of wheat by genetic transformation and elucidation of the role of this gene will be useful for understanding the mechanism underlying drought tolerance of Chinese wheat landrace PYXBM.

Abstract  In plants, basic leucine zipper (bZIP) transcription factors play important roles in regulatory processes, including stress response, pathogenic defense and light response as well as organ and tissue differentiation. Chinese wheat landrace Pingyaoxiaobaimai (PYXBM), an original parent of drought tolerant wheat varieties grown in northern China, is significantly tolerant to abiotic stresses such as drought, cold and nutrient deficiencies. In order to isolate key stress-responsive genes and then improve stress tolerances of conventional varieties, a bZIP transcription factor gene was isolated from a cDNA library of drought-treated PYXBM using the in situ plaque hybridization method, and was designated as Triticum aestivum L. abscisic acid (ABA)-responsive element binding protein 1 (TaABP1). It encodes 372 amino acids, and contains three conserved domains (C1-C3) in the N terminal and a bZIP domain in the C terminal which is a typical protein structure for the group member of bZIP family. Transcriptional activation analysis showed that TaABP1 activated the expression of downstream reporter genes in yeast without ABA application. TaABP1 protein fused with green fluorescent protein (GFP) demonstrated that the localization of TaABP1 protein is in the nucleus. Expression pattern assays indicated that TaABP1 was strongly induced by ABA, high salt, low temperature and drought, and its expression was stronger in stems and leaves than in the roots of wheat. Furthermore, overexpression of TaABP1 in tobacco showed significant improvement of drought tolerance. Data suggested that TaABP1 may be a good candidate gene for improving stress tolerance of wheat by genetic transformation and elucidation of the role of this gene will be useful for understanding the mechanism underlying drought tolerance of Chinese wheat landrace PYXBM.
Keywords:  wheat       expression pattern       stress tolerance       sub-cellular localization       transcriptional activation  
Received: 23 November 2011   Accepted:
Fund: 

The work was funded in part by the National Natural Science Foundation of China (31101147 and 30700508) and the National Key Project for Research on Transgenic Biology of China (2011ZX08002-002, 2011ZX08002-003 and 2011ZX08002-005), the National 863 Program of China (2008AA10Z124) .

Corresponding Authors:  Correspondence MA You-zhi, Tel/Fax: +86-10-82108789, E-mail: mayouzhi@yahoo.com.cn     E-mail:  mayouzhi@yahoo.com.cn

Cite this article: 

CAO Xin-you, CHEN Ming*, XU Zhao-shi, CHEN Yao-feng, LI Lian-cheng, YU Yue-hua, LIU Yangna, MA You-zhi. 2012. Isolation and Functional Analysis of the bZIP Transcription Factor Gene TaABP1 from a ChineseWheat Landrace. Journal of Integrative Agriculture, 12(10): 1580-1591.

[1]Abe H, Yamaguchi-Shinozaki K, Urao T, Iwasaki T,Hosokawa D, Shinozaki K. 1997. Role of ArabidopsisMYC and MYB homologs in drought- and abscisic acidregulatedgene expression. The Plant Cell, 9, 1859-1868.

[2]Abe M, Kobayashi Y, Yamamoto S, Daimon Y, YamaguchiA, Ikeda Y, Ichinoki H, Notaguchi M, Goto K, Araki T.2005. A bZIP protein mediating signals from the floralpathway integrator FT at the shoot apex. Science, 309,1052-1056.

[3]Agarwal P, Agarwal P, Reddy M, Sopory S. 2006. Role ofDREB transcription factors in abiotic and biotic stresstolerance in plants. Plant Cell Reports, 25, 1263-1274.

[4]Bartels D, Sunkar R. 2005. Drought and salt tolerance inplants. Critical Reviews in Plant Sciences, 24, 23-58.

[5]Choi H, Hong J, Kang J. 2000. ABFs, a family of ABAresponsiveelement binding factors. Journal ofBiological Chemistry, 21, 1723-1730.

[6]Chuang C F, Running M P, Williams R W, Meyerowitz E M.1999. The PERIANTHIA gene encodes a bZIP proteininvolved in the determination of floral organ number inArabidopsis thaliana. Genes and Development, 13,334-344.

[7]Despres C, DeLong C, Glaze S, Liu E, Fobert P R. 2000. TheArabidopsis NPR1/NIM1 protein enhances the DNAbinding activity of a subgroup of the TGA family ofbZIP transcription factors. The Plant Cell, 12, 279-290.

[8]Dubouzet J, Sakuma Y, Ito Y, Kasuga M, Doubouzet E,Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K.2003. OsDREB genes in rice, Oryza sativa L., encodetranscription activators that function in drought-, highsalt-and cold-responsive gene expression. The PlantJournal, 33, 751-763.

[9]Eulgem T, Somssich I E. 2007. Networks of WRKYtranscription factors in defense signaling. CurrentOpinion in Plant Biology, 4, 366-371.

[10]Finkelstein R R, Gampala S S, Rock C D. 2002.Abscisic acidsignaling in seeds and seedings. The Plant Cell, 14(Suppl.), 15-45.

[11]Finkelstein R R, Lynch T J. 2000. The Arabidopsis abscisicacid response gene ABI5 encodes a basic leucine zippertranscription factor. The Plant Cell, 12, 599-609.

[12]Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M, SekiM, Hiratsu K, Ohme-TakagiM, Shinozaki K, Yamaguchi-Shinozaki K. 2005. REB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhancesdrought stress tolerance in Arabidopsis. The PlantCell, 17, 3470-3488.

[13]Furihata T, Maruyama K, Fujita Y, Umezawa T, Yoshida R,Shinozaki K, Yamaguchi-Shinozaki K. 2006. Abscisicacid-dependent multisite phosphorylation regulates theactivity of a transcription activator AREB1. Proceedingsof the National Academy of Sciences of the UnitedStates of America, 103, 1988-1993.

[14]Hoekstra F A, Golovina E A, Buitink J. 2001. Mechanismsof plant desiccation tolerance. Trends in Plant Science,6, 431-438.

[15]Hossain MA, Cho J I, Han M H, Ahn C H, Jeon J S, An G H,Park P B. 2010. The ABRE-binding bZIP transcriptionfactor OsABF2 is a positive regulator of abiotic stressand ABA signaling in rice. Journal of Plant Physiology,167, 1512-1520.

[16]Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L. 2006.Over-expressing a NAM, ATAF, and CUC (NAC)transcription factor enhances drought resistance andsalt tolerance in rice. Proceedings of the NationalAcademy of Sciences of the United States of America,103, 12987-12992.

[17]Hurst H C. 1995. Transcription factors 1: bZIP proteins.Protein Profile, 2, 101-168.

[18]Iida K, Seki M, Sakurai T, Satou M, Akiyama K, Toyoda T,Konagaya A, Shinozaki K. 2005. RARTF: database andtools for complete sets of Arabidopsis transcriptionfactors. DNA Research, 12, 247-256.

[19]Iwata Y, Fedoroff NV, Koizumi N. 2008. Arabidopsis bZIP60is a proteolysis-activated transcription factor involvedin the endoplasmic reticulum stress response. The PlantCell, 20, 3107-3121.

[20]Jakoby M, Weisshaar B, Droge-Laser W, Vicente-CarbajosaJ, Tiedemann J, Kroj T, Parcy F. 2002. bZIP transcriptionfactors in Arabidopsis. Trends in Plant Science, 7, 106-111.

[21]Kaminaka H, Nake C, Epple P, Dittgen J, Schutze K, ChabanC, Holt B F, Merkle T, Schäfer E, Harter K, et al. 2006.bZIP10-LSD1 antagonism modulates basal defense andcell death in Arabidopsis following infection. The EMBOJournal, 25, 4400-4411.

[22]Kang J, Choi H, Kim S Y. 2002. Arabidopsis basic leucinezipper proteins that mediate stress-responsive abscisicacid signaling. The Plant Cell, 14, 343-357.

[23]KimS, Kang J Y, Cho D I, Park J H, Kim S Y. 2004. ABF2, anABRE-binding bZIP factor, is an essential componentof glucose signaling and its overexpression affectsmultiple stress tolerance. The Plant Journal, 40, 75-87.

[24]Kobayashi F, Maeta E, Terashima A, Kawaura K, OgiharaY, Takumi S. 2008. Development of abiotic stresstolerance via bZIP-type transcription factor LIP19 incommon wheat. Journal of Experimental Botany, 59,891-905.

[25]Kobayashi F, Maeta E, Terashima A, Takumi S. 2008.Positive role of a wheat HvABI5 ortholog in abioticstress response of seedlings. Plant Physiology, 134,74-86.

[26]Kurahashi Y, Terashima A, Takumi S. 2009. Variation indehydration tolerance, ABA sensitivity and related geneexpression patterns in D-genome progenitor andsynthetic hexaploid wheat lines. International Journalof Molecular Sciences, 10, 2733-2751.

[27]Landschulz W H, Johnson P F, McKnight S L. 1988. Theleucine zipper: a hypothetical structure common to anew class of DNA binding proteins. Science, 240, 1759-1764.

[28]Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. 1998. Two transcriptionfactors, DREB1 and DREB2, with an EREBP/AP2 DNAbindingdomain separate two cellular signaltransduction pathways in drought and low-temperatureresponsivegene expression in Arabidopsis. The PlantCell, 10, 1391-1406.

[29]Lu G J, Gao C X, Zheng X N, Han B. 2009. Identification ofOsbZIP72 as a positive regulator of ABA response anddrought tolerance in rice. Planta, 229, 605-615.

[30]Mukherjee K, Choudhury A R, Gupta B, Gupta S, SenguptaD N. 2006. An ABRE-binding factor, OsBZ8, is highlyexpressed in salt tolerant cultivars than in salt sensitivecultivars of indica rice. BMC Plant Biology, 6, 1-14.

[31]Nakagawa H, Ohmiya K, Hattori T. 1996.Arice bZIP protein,designated OsBZ8, is rapidly induced by abscisic acid.The Plant Journal, 9, 217-227.

[32]Niggeweg R, Thurow C, Kegler C, Gatz C. 2000. Tobaccotranscription factor TGA2.2 is the main component ofas-1-binding factor ASF-1 and is involved in salicylicacid- and auxin-inducible expression of as-1-containingtarget promoters.

[33]Journal of Biological Chemistry, 275,19897-19905.

[34]Nijhawan A, JainM, TyagiAM, Khurana J P. 2008. Genomicsurvey and gene expression analysis of the basic leucinezipper transcription factor family in rice. PlantPhysiology, 146, 333-350.

[35]Niu X, Renshaw-Gegg L, Miller L, Guiltinan M. 1999.Bipartite determinants of DNA-binding specificity ofplant basic leucine zipper protein. Plant MolecularBiology, 41, 1-13.

[36]O’shea E K, Rutkowski R, Kim P S. 1992. Mechanism ofspecificity in the Fos-Jun oncoprotein heterodimer. Cell,68, 699-708.

[37]Osterlund M T, Hardtke C S, Wei N, Deng X W. 2000.Targeted destabilization of HY5 during light-regulateddevelopment of Arabidopsis. Nature, 405, 462-466.

[38]Oyama T, Shimura Y, Okada K. 1997. The Arabidopsis HY5gene encodes a bZIP protein that regulates stimulusinduceddevelopment of root and hypocotyls. GenesDevelopment, 11, 2983-2995.

[39]Pellegrineschi A, Reynolds M, Pacheco M, Brito R M,Almeraya R, Yamaguchi-Shinozaki K, Hoisington D.2004. Stress-induced expression in wheat of theArabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions.Genome, 47, 493-500.

[40]Pontier D, Miao Z H, Lam E. 2001. Trans-dominantsuppression of plant TGA factors reveals their negativeand positive roles in plant defense responses. ThePlant Journal, 27, 529-538.

[41]Rodriguez-Uribe L, O’Connell M A. 2006. A root-specificbZIP transcription factor is responsive to water deficitstress in tepary bean (Phaseolus acutifolius) andcommon bean (P. vulgaris). Journal of ExperimentalBotany, 57, 1391-1398.

[42]Schmidt R J, Burr FA, Aukerman M J, Burr B. 1990. Maizeregulatory gene opaque-2 encodes a protein with a‘leucine zipper’ motif that binds to zein DNA.

[43]Proceedings of the National Academy of Sciences ofthe United States of America, 87, 46-50.

[44]Schmidt R J, Burr F A, Burr B. 1987. Transposon taggingand molecular analysis of the maize regulatory locusopaque-2.

[45]Science, 238, 960-963.

[46]Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y,Kamiya A, Nakajima M, Enju A, Sakurai T, et al. 2002.Monitoring the expression profiles of 7000 Arabidopsisgenes under drought, cold and high salinity stressesusing a full-length cDNA microarray. The PlantJournal, 31, 279-292.

[47]Shimizu H, Sato K, Berberich T,MiyazakiA, Ozaki R, Imai R,Kusano T. 2005. LIP19, a basic region leucine zipperprotein, is a Fos-like molecular switch in the cold signalingof rice plants. Plant Cell Physiology, 46, 1623-1634.

[48]Thomashow M F. 1999. Plant cold acclimation: freezingtolerance genes and regulatory mechanisms. AnnualReview of Plant Physiology and Plant MolecularBiology, 50, 571-599.

[49]Thurow C, Schiermeyer A, Krawczyk S, Butterbrodt T,Nickolov K, Gatz C. 2005. Tobacco bZIP transcriptionfactor TGA2.2 and related factor TGA2.1 have distinctroles in plant defense responses and plan tdevelopment. Plant Journal, 44, 100-113.

[50]Ulm R, Baumann A, Oravecz A, Mate Z, Adam E, Oakeley EJ, Schafer E, Nagy F. 2004. Genome-wide analysis of geneexpression reveals function of the bZIP transcriptionfactor HY5 in the UV-B response of Arabidopsis.Proceedings of the National Academy of Sciences ofthe United States of America, 101, 1397-1402.

[51]Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K,Yamaguchi-Shinozaki K. 2000. Arabidopsis basicleucine zipper transcription factors involved in anabscisic acid-dependent signal transduction pathwayunder drought and high-sa l ini ty condi tions .Proceedings of the National Academy of Sciences ofthe United States of America, 97, 11632-11637.

[52]Walsh J, Waters C A, Freeling M. 1998. The maize geneliguleless2 encodes a basic leucine zipper proteininvolved in the establishment of the leaf blade-sheathboundary. Genes and Development, 12, 208-218.

[53]Wellmer F, Kircher S, Rugner A, Frohnmeyer H, Schafer E,Harter K. 1999. Phosphorylation of the parsley bZIPtranscription factor CPRF2 is regulated by light. Journalof Biological Chemistry, 274, 29476-29482.

[54]Weltmeier F, Rahmani F, Ehlert A, Dietrich K, Schütze K,Wang X, Chaban C, Hanson J, Teige M, Harter K, et al.2009. Expression patterns within the Arabidopsis C/S1bZIP transcription factor network: availability ofheterodimerization partners controls gene expressionduring stress response and development. PlantMolecular Biology, 69, 107-119.

[55]Wingender E, Chen X, Fricke E, Geffers R, Hehl R, LeibichI, Krull M, Matys V, Michael H, Ohnhauser R, et al.2001. The TRANSFAC system on gene expressionregulation. Nucleic Acids Research, 29, 281-283.

[56]Xiang Y, Tang N, Du H, Ye HY, Xiong L Z H. 2008.Characterization of OsbZIP23 as a key player of the basicleucine zipper transcription factor family for conferringabscisic acid sensitivity and salinity and droughttolerance in rice. Plant Physiology, 148, 1938-1952.

[57]Zhang B, Foley R C, Singh K B. 1993. Isolation andcharacterization of two related Arabidopsis ocs-elementbZIP binding proteins. The Plant Journal, 4, 711-716.

[58]Zhu J K. 2002. Salt and drought stress signal transductionin plants. Annual Review of Plant Biology, 53, 247-273.
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