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| Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle |
| WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong |
| Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University,Yangling 712100, P.R.China |
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摘要 Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds (Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein) using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed: W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium (P>0.05). The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD (P<0.01 or P<0.05). These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.
Abstract Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds (Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein) using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed: W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium (P>0.05). The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD (P<0.01 or P<0.05). These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.
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Received: 04 July 2011
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (30972080), the Keystone Project of Transfer Gene in China (2009ZX08009-157B), and the Program of National Beef Cattle Industrial Technology System of China (CARS-38). |
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Corresponding Authors:
Correspondence CHEN Hong, E-mail: chenhong1212@263.net
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Cite this article:
WANG Mou, ZHANG Ya, YU Hui, LAI Xin-sheng, ZHU Jin-long, JIAO Jin-zhen, LAN Xian-yong, LEI Chuzhao, ZHANG Liang-zhi, CHEN Hong.
2012.
Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle. Journal of Integrative Agriculture, 12(8): 1327-1332.
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