|
|
|
|
|
| Identification of Organic Substances Associated with Tissue Senescence in Upland Cotton (Gossypium spp. L.) Based on GC-MS Analysis |
| College of Agronomy, Agricultural University of Hebei |
|
|
|
摘要 Premature senescence in crop production, especially occurred at the late growth stage, generally results in a reduction inyield and quality. Therefore, it is beneficial for yield and quality to properly delay senescence of plant tissues during thelate developmental stage. In this study, it was observed that the chlorophyll content and photosynthetic rate weregradually decreased along leaf growth progression, and the rates of reduction were promoted by drought. Based on gaschromatography-mass spectrometry (GC-MS) analysis, total eight, five, seven, and five kinds of organic compounds thatputatively associated with the tissue senescent progression were identified in leaves, fruit branches, petals, and sepals,respectively. It was found that the identified organic compound, such as α-pinene, β-pinene, and pentadecane werepresent in different tissues. Among the total ten organic substances identified to be related with the leaf senescence, halfwere specifically detected in the drought treatment. These results suggest some biochemical pathways associated withthe leaf senescence are distinctly regulated by drought. The identified organic compounds in the tested tissues showedthree types on the performance pattern based on the contents along with the senescent progression, including graduallyincreasing, decreasing, and a curve with one single peak. Thus, during the senescence process in tissues, a subset ofmetabolic substances occur modifications on the quantities, reflecting a complicate biochemical reactions are initiated viathe senescence signals. Further analysis of the important organic substances will be helpful for elucidation of the tissuesenescence mechanism at the biochemical level and provide a new insight of the senescence signaling transductions incotton.
Abstract Premature senescence in crop production, especially occurred at the late growth stage, generally results in a reduction inyield and quality. Therefore, it is beneficial for yield and quality to properly delay senescence of plant tissues during thelate developmental stage. In this study, it was observed that the chlorophyll content and photosynthetic rate weregradually decreased along leaf growth progression, and the rates of reduction were promoted by drought. Based on gaschromatography-mass spectrometry (GC-MS) analysis, total eight, five, seven, and five kinds of organic compounds thatputatively associated with the tissue senescent progression were identified in leaves, fruit branches, petals, and sepals,respectively. It was found that the identified organic compound, such as α-pinene, β-pinene, and pentadecane werepresent in different tissues. Among the total ten organic substances identified to be related with the leaf senescence, halfwere specifically detected in the drought treatment. These results suggest some biochemical pathways associated withthe leaf senescence are distinctly regulated by drought. The identified organic compounds in the tested tissues showedthree types on the performance pattern based on the contents along with the senescent progression, including graduallyincreasing, decreasing, and a curve with one single peak. Thus, during the senescence process in tissues, a subset ofmetabolic substances occur modifications on the quantities, reflecting a complicate biochemical reactions are initiated viathe senescence signals. Further analysis of the important organic substances will be helpful for elucidation of the tissuesenescence mechanism at the biochemical level and provide a new insight of the senescence signaling transductions incotton.
|
|
Received: 27 July 2010
Accepted:
|
|
Corresponding Authors:
Correspondence XIAO Kai, Professor, Ph D, Mobile: 13784984637, E-mail: xiaokai@hebau.edu.cn
E-mail: xuzl@hebau.edu.cn
|
| About author: XU Zhen-long, MSc, Tel: +86-312-7528416, E-mail: xuzl@hebau.edu.cn |
Cite this article:
XU Zhen-long, GUO Cheng-jin, GU Jun-tao, LU Wen-jing, LI Xiao-juan, XIAO Kai.
2011.
Identification of Organic Substances Associated with Tissue Senescence in Upland Cotton (Gossypium spp. L.) Based on GC-MS Analysis. Journal of Integrative Agriculture, 10(8): 1197-1205.
|
| [1] Dhindsa R A, Plumb-Dhindsa P, Thorpe T A. 1981. Leafsenescence: correlated with increased permeability and lipidperoxidation, and decreased levels of superoxide dismutaseand catalase. Journal of Experimental Botany, 126, 93-101.[2] Diaz C, Purdy S, Christ A, Morot-Gaudry J F, Wingler A,Masclaux-Daubresse C. 2005. Characterization of markersto determine the extent and variability of leaf senescence inArabidopsis. A metabolic profiling approach. PlantPhysiology, 138, 898-908.[3] Dong H, Li W, Tang W, Li Z, Zhang D, Niu Y. 2006. Yield,quality and leaf senescence of cotton grown at varyingplanting dates and plant densities in the Yellow River Valleyof China. Field Crops Research, 98, 106-115.[4] Dong H, Niu Y, Kong X, Luo Z. 2009. Effects of early-fruitremoval on endogenous cytokinins and abscisic acid in relationto leaf senescence in cotton. Plant Growth Regulation, 59,93-101.[5] Dong H, Niu Y, Li W, Zhang D. 2008. Effects of cotton rootstockon endogenous cytokinins and abscisic acid in xylem sap andleaves in relation to leaf senescence. Journal of ExperimentalBotany, 59, 1295-1304.[6] Dong H, Li W, Tang W, Li Z, Zhang D. 2007. Heterosis in yield,endotoxin expression and some physiological parameters inBt transgenic cotton. Plant Breeding, 126, 169-175.[7] Gan S, Amasino R M. 1997. Making sense of senescence. PlantPhysiology, 113, 313-319.[8] Gribic V, Bleecker A B. 1995. Ethylene regulates the timing ofleaf senescence in Arabidopsis. The Plant Journal, 8, 595-602.[9] Guinn G, Brummett D. 1993. Leaf age, decline in photosynthesis,and changes in abscisic acid, indole-3-acetic acid and cytokininin cotton leaves. Field Crops Research, 32, 269-275.[10] Hare P D, Møller S G, Huang L F, Chua N H. 2003. LAF3, anovel factor required for normal phytochrome A signaling.Plant Physiology, 133, 1592-1604.[11] Himelblau E, Amasino R M. 2001. Nutrients mobilized fromleaves of Arabidopsis thaliana during leaf senescence. Journalof Plant Physiology, 158, 1317-1323.[12] Hörtensteiner S, Feller U. 2002. Nitrogen metabolism andremobilization during senescence. Journal of ExperimentalBotany, 53, 927-937.[13] Koch T, Krumm T, Jung V, Engelberth J, Boland W. 1999.Differential induction of plant volatile biosynthesis in thelima bean by early and late intermediates of the octadecanoidsignalingpathway. Plant Physiology, 121, 153-162.[14] Lim P O, Woo H R, Nam H G. 2003. Molecular genetics of leafsenescence in Arabidopsis. Trends in Plant Science, 8, 272- 278. Liu L, Li C, Sun H, Jia L. 2006. Advances of research on cottonleaf senescence physiology. Chinese Agricultural ScienceBulletin, 22, 316-321. (in Chinese)[15] Liu L, Li C, Sun Hn, Zhang Y, Bai Z, Feng L. 2009. Effects ofnitrogen on cotton senescence and the correspondingphysiological mechanisms. Scientia Agricultura Sinica, 42,1575-1581. (in Chinese)[16] Lohoman K N, Gan S S, John M C. 1994. Molecular analysis ofnatural leaf senescence in Arabidopsis thaliana. PhysiologiumPlant, 92, 322-328.[17] Mae T. 2004. Leaf senescence and nitrogen metabolism. In:Noodén L D, ed., Plant Cell Death Processes. Elsevier,Amsterdam. pp. 157-168.[18] Masclaux-Daubresse C, Purdy S, Lemaitre T, Pourtau N, TaconnatL, Renou J, Wingler A. 2006. Genetic variation suggestsinteraction between cold acclimation and metabolic regulationof leaf senescence. Plant Physiology, 143, 434-446.[19] Nam H G. 1997. The molecular genetic analysis of leaf senescence.Current Opinion in Biotechnology, 8, 200-207.[20] Pic E, Bernard Serve T, Tardieu F, Turc O. 2002. Leaf senescenceinduced by mild water deficit follows the same sequence ofmacroscopic, biochemical, and molecular events asmonocarpic senescence in pea. Plant Physiology, 128, 236-246.[21] Porra R J, Thompson W A, Kreidemann P E. 1989. Determinationof accurate extinction coefficients and simultaneous equationsfor assaying chlorophylls a and b extracted with four differentsolvents: verification of the concentration of chlorophyllstandards by atomic absorption spectroscopy. Biochimicaet Biophysica Acta, 975, 348-394.[22] Seltmann M A, Stingl N E, Lautenschlaeger J K, Krischke M,Mueller M J, Berger S. 2010. Differential impact oflipoxygenase 2 and jasmonates on natural and stress-inducedsenescence in Arabidopsis. Plant Physiology, 152, 1940-1950.[23] Shen F, Yu S, Fan S, Li J, Huang Z. 2003. The relationshipbetween hormone and membrance lipid peroxidation in cottonleaf during senescence. Acta Photophysiologica Sinica, 29,589-592. (in Chinese)[24] Smart C M. 1994. Gene expression during leaf senescence. NewPhytologist, 126, 419-448.[25] Vicentini F, Hortensteiner S, Schellenberg M. 1995. Chlorophyllbreakdown in senescence leaves: identification of thebiochemical lesion in a stay-green genotype of Festucapratensis huds. New Phytologist, 129, 247-252.[26] Wright P R. 1998. Research into early senescence syndrome incotton. Better Crops International, 12, 14-16.[27] Wright P R. 1999. Premature senescence of cotton (Gossypiumhirsutum L.): predominantly a potassium disorder causedby an imbalance of source and sink. Plant and Soil, 211, 231-239.[28] Zhang H, Li C, Xiao K. 2008. The effects of nitrogen onphotosynthesis and activities of cellular protection enzymesin cotton cultivars with different senescing properties. ActaAgriculturae Boreali-Sinica, 23, 170-174. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
