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Journal of Integrative Agriculture  2026, Vol. 25 Issue (5): 1813-1821    DOI: 10.1016/j.jia.2024.07.029
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification and fine mapping of a major QTL for grain protein content, qGPC4D, using wheat–Aegilops tauschii introgression lines
Yijun Wang*, Jinhao Han*, Tenglong Zhang, Mengjia Sun, Hongyu Ren, Cunyao Bo, Yuqing Diao, Xin Ma, Hongwei Wang, Xiaoqian Wang#

National Key Laboratory of Wheat Improvement/College of Agronomy, Shandong Agricultural University, Tai’an 271018, China

 Highlights 
A major and stable quantitative trait locus (QTL) for grain protein content, qGPC4D, was identified from Aegilops tauschii and fine-mapped to a 9.88 Mb interval.
High-throughput kompetitive allele-specific PCR (KASP) markers were developed for qGPC4D, enabling efficient marker-assisted selection in breeding.
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摘要  小麦是一种重要的粮食作物,对粮食安全和人类健康至关重要。提高小麦品质已成为育种家满足市场日益增长的需求的重要任务。本研究以高产小麦品种济麦22和粗山羊草Y215为亲本构建了一套小麦-粗山羊草渐渗系。利用55K SNP芯片鉴定群体基因型并筛选出2727个高质量的SNP,同时测定两个环境下的籽粒品质相关性状。通过QTL分析鉴定出8个与籽粒蛋白质含量、淀粉含量和湿面筋含量相关的QTL。其中,qGPC4D为具有环境稳定性的主效QTL,其有利等位基因来自粗山羊草Y215。随后,利用渐渗系进一步精细定位,将qGPC4D缩小到9.88 Mb的物理区间内,并开发了三个连锁的KASP标记。本试验为小麦优质育种提供了重要的候选基因、优良的渐渗系和连锁的KASP标记。

Abstract  

Wheat is a staple cereal crop that is crucial for food security and human health.  Improving wheat quality has become an essential task for allowing breeders to meet escalating market demand.  In this study, a set of wheat–Aegilops tauschii introgression lines was developed from a cross between the high-yielding wheat variety Jimai 22 and Ae. tauschii Y215.  A high-density genetic map containing 2,727 single nucleotide polymorphisms (SNPs) was constructed using a 55K SNP array, and it was used to conduct a quantitative trait loci (QTLs) analysis for grain quality-related traits.  Eight QTLs were identified for grain protein content (GPC), starch content, and wet gluten content in two environments.  Among them, a major and environmentally stable QTL for GPC, qGPC4D, was identified, with favorable alleles contributed by Ae. tauschii Y215.  Subsequently, qGPC4D was narrowed down to a 9.88 Mb physical interval through further fine mapping utilizing the introgression lines.  In addition, three linked SNP of qGPC4D were converted into high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the introgression population.  These findings offer promising candidate genes, elite introgression lines, and KASP markers for high-quality wheat breeding.  

Keywords:  wheat        Aegilops tauschii        protein content        QTL        KASP  
Received: 12 April 2024   Accepted: 13 June 2024 Online: 19 July 2024  
Fund: This work was supported by Shandong Province Agricultural Fine Seeds Project, China (2022LZGC005), the National Natural Science Foundation of China (31901491 and 32030081), and the Young and Innovative Team Plan of Shandong Higher Education Institutions, China (2023KJ336).
About author:  #Correspondence Xiaoqian Wang, E-mail: xqwang1988@126.com * These authors contributed equally to this study.

Cite this article: 

Yijun Wang, Jinhao Han, Tenglong Zhang, Mengjia Sun, Hongyu Ren, Cunyao Bo, Yuqing Diao, Xin Ma, Hongwei Wang, Xiaoqian Wang. 2026. Identification and fine mapping of a major QTL for grain protein content, qGPC4D, using wheat–Aegilops tauschii introgression lines. Journal of Integrative Agriculture, 25(5): 1813-1821.

Belanger F C, Kriz A L. 1991. Molecular basis for allelic polymorphism of the maize Globulin-1 gene. Genetics129, 863–872.

Bo C, Fan Z, Ma X, Li A, Wang H, Kong L, Wang X. 2022. Identification and introgression of a novel HMW-GS gene from Aegilops tauschiiAgronomy12, 2709.

Borrill P, Ramirez-Gonzalez R, Uauy C. 2016. expVIP: A customizable RNA-seq data analysis and visualization platform  Plant Physiology170, 2172–2186.

Brenchley R, Spannagl M, Pfeifer M, Barker G L, D’amore R, Allen A M, Mckenzie N, Kramer M, Kerhornou A, Bolser D. 2012. Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature491, 705.

Cox T S, Sorrells M, Bergstrom G, Sears R, Gill B, Walsh E, Leath S, Murphy J. 1994. Registration of KS92WGRC21 and KS92WGRC22 hard red winter wheat germplasms resistant to wheat spindle-streak mosaic virus, wheat soilborne mosaic virus, and powdery mildew. Crop Science34, 546.

Cox T S, Wu J, Wang S, Cai J, Zhong Q, Fu B. 2017. Comparing two approaches for introgression of germplasm from Aegilops tauschii into common wheat. The Crop Journal5, 355–362.

Gaurav K, Arora S, Silva P, Sánchez-Martín J, Horsnell R, Gao L, Brar G S, Widrig V, John Raupp W, Singh N, Wu S, Kale S M, Chinoy C, Nicholson P, Quiroz-Chávez J, Simmonds J, Hayta S, Smedley M A, Harwood W, Pearce S, et al. 2021. Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement. Nature Biotechnology40, 422–431.

Ghaffary S M T, Faris J D, Friesen T L, Visser R G, Van Der Lee T A, Robert O, Kema G H. 2012. New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat. Theoretical and Applied Genetics124, 125–142.

Gill B, Wilson D, Raupp W, Hatchett J, Harvey T, Cox T, Sears R. 1991. Registration of KS89WGRC4 hard red winter wheat germplasm with resistance to Hessian fly, greenbug, and soil-borne mosaic virus. Crop Science31, 246.

Gill H S, Li C, Sidhu J S, Liu W, Sehgal S K. 2019. Fine mapping of the wheat leaf rust resistance gene Lr42International Journal of Molecular Sciences20, doi: 10.3390/ijms20102445.

Guo Y, Wang G, Guo X, Chi S, Yu H, Jin K, Huang H, Wang D, Wu C, Tian J, Chen J, Bao Y, Zhang W, Deng Z. 2023. Genetic dissection of protein and starch during wheat grain development using QTL mapping and GWAS. Frontiers in Plant Science14, 1189887.

Hao M, Zhang L Q, Zhao L B, Dai S F, Li A L, Yang W Y, Xie D E, Li Q C, Ning S Z, Yan Z H. 2019. A breeding strategy targeting the secondary gene pool of bread wheat: Introgression from a synthetic hexaploid wheat. Theoretical and Applied Genetics132, 2285–2294.

Hao Y C, Kong F M, Wang L L, Zhao Y, Li M Y, Che N X, Li S, Wang M, Hao M, Zhang X, Zhao Y. 2024. Genome-wide association study of grain micronutrient concentrations in bread wheat. Journal of Integrative Agriculture23, 1468–1480.

Harrell F E. 2019. Hmisc: harrell miscellaneous, R package version 4.2-0. https://CRAN.R-project.org/package=Hmisc

He F, Pasam R, Shi F, Kant S, Keeble-Gagnere G, Kay P, Forrest K, Fritz A, Hucl P, Wiebe K, Knox R, Cuthbert R, Pozniak C, Akhunova A, Morrell P L, Davies J P, Webb S R, Spangenberg G, Hayes B, Daetwyler H, et al. 2019. Exome sequencing highlights the role of wild-relative introgression in shaping the adaptive landscape of the wheat genome. Nature Genetics51, 896–904.

Huang L, Brooks S A, Li W, Fellers J P, Trick H N, Gill B S. 2003. Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics164, 655–664.

Imtiaz M, Ogbonnaya F C, Oman J, van Ginkel M. 2008. Characterization of quantitative trait loci controlling genetic variation for preharvest sprouting in synthetic backcross-derived wheat lines. Genetics178, 1725–1736.

Jiang P, Zhang P, Wu L, He Y, Li C, Ma H, Zhang X. 2021. Linkage and association mapping and kompetitive allele-specific PCR marker development for improving grain protein content in wheat. Theoretical and Applied Genetics134, 3563–3575.

Jiang Y, Wang D L, Hao M, Zhang J, Liu D C. 2023. Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters. Journal of Integrative Agriculture22, 999–1008.

Juliana P, Poland J, Huerta-Espino J, Shrestha S, Crossa J, Crespo-Herrera L, Toledo F H, Govindan V, Mondal S, Kumar U, Bhavani S, Singh P K, Randhawa M S, He X, Guzman C, Dreisigacker S, Rouse M N, Jin Y, Pérez-Rodríguez P, Montesinos-López O A, et al. 2019. Improving grain yield, stress resilience and quality of bread wheat using large-scale genomics. Nature Genetics51, 1530–1539.

Kerber E R. 1987. Resistance to leaf rust in hexaploid wheat: Lr32 a third gene derived from Triticum tauschiiCrop Science27, 204–206.

Kumar A, Mantovani E E, Simsek S, Jain S, Elias E M, Mergoum M. 2019. Genome wide genetic dissection of wheat quality and yield related traits and their relationship with grain shape and size traits in an elite×non-adapted bread wheat cross. PLoS ONE14, e0221826.

Kunert A, Naz A A, Dedeck O, Pillen K, Léon J. 2007. AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides×T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theoretical and Applied Genetics115, 683–695.

Li J, Wei H, Hu X, Li C, Tang Y, Liu D, Yang W. 2011. Identification of a high-yield introgression locus in Chuanmai 42 inherited from synthetic hexaploid wheat. Acta Agronomica Sinica37, 255–261. (in Chinese)

Li L L, Mao Z Q, Wang P, Cai J, Zhou Q, Zhong Y X, Jiang D, Wang X. 2025. Drought priming enhances wheat grain starch and protein quality under drought stress during grain filling. Journal of Integrative Agriculture24, 2888–2901.

Li N, Miao Y, Ma J, Zhang P, Chen T, Liu Y, Che Z, Shahinnia F, Yang D. 2023. Consensus genomic regions for grain quality traits in wheat revealed by Meta-QTL analysis and in silico transcriptome integration. The Plant Genome16, e20336.

Liu Z H, Lai X J, Chen Y J, Zhao P, Wang X M, Ji W Q, Xu S B. 2024. Selection and application of four QTLs of grain protein content in modern wheat cultivars. Journal of Integrative Agriculture23, 2557–2570.

Loit E, Melnyk C W, Macfarlane A J, Scott F W, Altosaar I. 2009. Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin. BMC Plant Biology9, 93.

Lutz J, Hsam S L K, Limpert E, Zeller F J. 1995. Chromosomal location of powdery mildew resistance genes in Triticum aestivum L. (common wheat). 2. Genes Pm2 and Pm19 from Aegilops squarrosa L. Heredity74, 152–156.

Lv G, Tian Q, Zhang F, Chen J, Niaz M, Liu C, Hu H, Sun C, Chen F. 2021. Reduced expression of lipoxygenase genes improves flour processing quality in soft wheat. Journal of Experimental Botany72, 6247–6259.

Ma F F, Li R Z, Guo G H, Nie F, Zhu L L, Liu W J, Lyu L, Bai S L, Zhao X P, Li Z, Zhang D L, Li H, Li S P, Zhou Y, Song C P. 2023. Introgression of QTL from Aegilops tauschii enhances yield-related traits in common wheat. The Crop Journal11, 1521–1532.

Mahjourimajd S, Taylor J, Rengel Z, Khabaz-Saberi H, Kuchel H, Okamoto M, Langridge P. 2016. The genetic control of grain protein content under variable nitrogen supply in an Australian wheat mapping population. PLoS ONE11, e0159371.

Marcone M F, Kakuda Y, Yada R Y. 1998. Salt-soluble seed globulins of various dicotyledonous and monocotyledonous plants - I. Isolation/purification and characterization. Food Chemistry62, 27–47.

Marcussen T, Sandve S R, Heier L, Spannagl M, Pfeifer M, Jakobsen K S, Wulff B, Steuernagel B, Mayer K, Olsen O A. 2014. Ancient hybridizations among the ancestral genomes of bread wheat. Science345, 1250092.

Miranda L M, Murphy J P, Marshall D, Cowger C, Leath S. 2007. Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theoretical and Applied Genetics114, 1451–1456.

Miranda L M, Murphy J P, Marshall D, Leath S. 2006. Pm34: A new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theoretical and Applied Genetics113, 1497.

Mirzaghaderi G, Mason A S. 2019. Broadening the bread wheat D genome. Theoretical and Applied Genetics132, 1295–1307.

Muqaddasi Q H, Brassac J, Ebmeyer E, Kollers S, Korzun V, Argillier O, Stiewe G, Plieske J, Ganal M W, Röder M S. 2020. Prospects of GWAS and predictive breeding for European winter wheat’s grain protein content, grain starch content, and grain hardness. Scientific Reports10, 12541.

Ogbonnaya F C, Abdalla O, Mujeeb-Kazi A, Kazi A G, Xu S S, Gosman N, Lagudah E S, Bonnett D, Sorrells M E, Tsujimoto H. 2013. Synthetic hexaploids: harnessing species of the primary gene pool for wheat improvement. Plant Breeding Reviews37, 35–122.

Olson E L, Rouse M N, Pumphrey M O, Bowden R L, Gill B S, Poland J A. 2013. Introgression of stem rust resistance genes SrTA10187 and SrTA10171 from Aegilops tauschii to wheat. Theoretical & Applied Genetics126, 2477–2484.

Pu Z E, Ye X L, Li Y, Shi B X, Guo Z, Dai S F, Ma J, Liu Z H, Jiang Y F, Li W, Jiang Q T, Chen G Y, Wei Y M, Zheng Y L. 2022. Identification and validation of novel loci associated with wheat quality through a genome-wide association study. Journal of Integrative Agriculture21, 3131–3147.

Rasheed A, Ogbonnaya F C, Lagudah E, Appels R, He Z. 2018. The goat grass genome’s role in wheat improvement. Nature Plants4, 56–58.

Raupp W J, Sukhwinder-Singh, Brown-Guedira G L, Gill B S. 2001. Cytogenetic and molecular mapping of the leaf rust resistance gene Lr39 in wheat. Theoretical and Applied Genetics102, 347–352.

Rowland G G, Kerber E R. 1974. Telocentric mapping in hexaploid wheat of genes for leaf rust resistance and other characters derived from Aegilops squarrosaCanadian Journal of Genetics & Cytology16, 137–144.

Rudd J C, Devkota R N, Baker J A, Peterson G L, Lazar M D, Bean B, Worrall D, Baughman T, Marshall D, Sutton R, Rooney L W, Nelson L R, Fritz A K, Weng Y, Morgan G D, Seabourn B W. 2014. ‘TAM 112’ wheat, resistant to greenbug and wheat curl mite and adapted to the dryland production system in the southern high plains. Journal of Plant Registrations8, 291–297.

Saini D K, Devi P, Kaushik P. 2020. Advances in genomic interventions for wheat biofortification: A review. Agronomy10, 62.

Sambasivam P, John M, Michael A, Urmil B, Xiaojing W, Li H, Karin D, Mingcheng L, Xiuying K, Harbans B. 2013. The gene Sr33, an ortholog of barley Mla genes, encodes resistance to wheat stem rust race Ug99. Science341, 786–788.

Shang J, Li L, Liu C, Hong J, Liu M, Zhao B, Zheng X. 2021. Relationships of flour characteristics with isolated starch properties in different Chinese wheat varieties. Journal of Cereal Science99, 103210.

Shevkani K, Katyal M, Singh N. 2024. A comparative review of protein and starch characteristics and end-use quality of soft and hard wheat. Food Chemistry Advances4, 100613.

Singh R P, Nelson J C, E. S M. 2000. Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Science40, 1148–1155.

Song Z P, Zuo Y Y, Xiang Q, Li W J, Li J, Liu G, Dai S F, Yan Z H. 2023. Investigation of Aegilops umbellulata for stripe rust resistance, heading date, and the contents of iron, zinc, and gluten protein. Journal of Integrative Agriculture22, 1258–1265.

Su Y, Liao P, Song D, Huang S, He J, Gao X, Li S. 2020. Application of Aegilops tauschiiTriticum aestivum recombinant inbred lines for grain protein content quantitative trait loci detection and wheat improvement. Canadian Journal of Plant Science100, 425–434.

Uthayakumaran S, Wrigley C W. 2010. Wheat: Characteristics and quality requirements. In: Wrigley C W, Batey I L, eds., Cereal Grains: Assessing and Managing Quality. Woodhead Publishing/CRC Press, Cambridge, UK. pp. 59–111.

Wang X, Yan X, Tian X, Zhang Z, Wu W, Shang J, Ouyang J, Yao W, Li S. 2020. Glycine- and proline-rich protein OsGPRP3 regulates grain size and quality in rice. Journal of Agricultural and Food Chemistry68, 7581–7590.

Wang Z, Li Y, Yang Y, Liu X, Qin H, Dong Z, Zheng S, Zhang K, Wang D. 2017. New insight into the function of wheat glutenin proteins as investigated with two series of genetic mutants. Scientific Reports7, 3428.

Wiersma A T, Pulman J A, Brown L K, Cowger C, Olson E L. 2017. Identification of Pm58 from Aegilops tauschiiTheoretical and Applied Genetics130, 1123–1133.

Yan L, Liang F, Xu H, Zhang X, Zhai H, Sun Q, Ni Z. 2017. Identification of QTL for grain size and shape on the D genome of natural and synthetic allohexaploid wheats with near-identical AABB genomes. Frontiers in Plant Science8, 1705.

Yang Z, Wang Z, Hu Z, Xin M, Yao Y, Peng H, You M, Su Z, Guo W. 2020. Comparative analysis of the genomic sequences between commercial wheat varieties Jimai 22 and Liangxing 99. Acta Agronomica Sinica46, 1870–1883. (in Chinese)

Yu M, Chen G Y, Zhang L Q, Liu Y X, Liu D C, Wang J R, Pu Z E, Zhang L, Lan X J, Wei Y M, Liu C J, Zheng Y L. 2014. QTL mapping for important agronomic traits in synthetic hexaploid wheat derived from Aegiliops tauschii ssp. tauschiiJournal of Integrative Agriculture13, 1835–1844.

Zhang G, Chen R Y, Shao M, Bai G, Seabourn B W. 2021. Genetic analysis of end-use quality traits in wheat. Crop Science61, 1709–1723.

Zhang J, Ren J, Yang J, Fu S, Zhang X, Xia C, Zhao H, Yang K, Wen C. 2023. Evaluation of SNP fingerprinting for variety identification of tomato by DUS testing. Agriculture Communications1, 100006.

Zhang L, Guan E, Yang Y, Liu Y, Zhang T, Bian K. 2021. Impact of wheat globulin addition on dough rheological properties and quality of cooked noodles. Food Chemistry362, 130170.

Zhang L, Guan E, Zhang K, Zhang T, Bian K. 2022. The aggregation characteristics of wheat globulin induced by heating and edible salts and its effects on noodle processing quality. LWT154, 112803.

Zhao G, Xie S, Zong S, Wang T, Mao C, Shi J, Li J. 2022. Mutation of TL1, encoding a novel C2H2 zinc finger protein, improves grains eating and cooking quality in rice. Theoretical and Applied Genetics135, 3531–3543.

Zheng F F, Deng Z Y, Shi C L, Zhang X Y, Tian J C. 2013. QTL mapping for dough mixing characteristics in a recombinant inbred population derived from a waxy × strong gluten wheat (Triticum aestivum L.). Journal of Integrative Agriculture12, 951–961.

Zhou Y, Bai S, Li H, Sun G, Zhang D, Ma F, Zhao X, Nie F, Li J, Chen L, Lv L, Zhu L, Fan R, Ge Y, Shaheen A, Guo G, Zhang Z, Ma J, Liang H, Qiu X, et al. 2021. Introgressing the Aegilops tauschii genome into wheat as a basis for cereal improvement. Nature Plants7, 774–786.

Zhu C, Wang C, Zhou J, Wang Y, Chen Q, Fu L. 2022. Purification and identification of globulin-1 S allele as a novel allergen with N-glycans in wheat (Triticum aestivum). Food Chemistry390, 133189.

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