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Special Issue:
动物科学合辑Animal Science
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| MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle |
Hong Quyen Dang1, 2, XU Gu-li1, HOU Lian-jie1, XU Jian1, HONG Guang-liang1, Chingyuan Hu3, WANG Chong1 |
1 National Engineering Research Center for Breeding Swine Industry/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China
2 Bac Giang Agricultural and Forestry University, Bac Giang 26000, Viet Nam
3 Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Honolulu, HI 96822, USA |
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Abstract Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. MicroRNAs (miRNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. miR-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated miR-22 function in proliferation and differentiation of porcine satellite cells (PSCs) in skeletal muscle. Our data show that miR-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated (P<0.05) during differentiation. After treated with the miR-22 inhibitor, PSCs proliferation was significantly increased (P<0.05), as indicated by the up-regulation (P<0.01) of cyclin D1 (CCND1), cyclin B1 (CCNB1) and down-regulation (P<0.05) of P21. Conversely, over-expression of miR-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed (P<0.05), evidenced by two major myogenic markers: myogenin (MyoG) and myosin heavy chain (MyHC), after transfecting the PSCs with miR-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with miR-22 mimics. In conclusion, the data from this study indicated that miR-22 inhibited the PSCs proliferation but promoted their differentiation.
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Received: 25 October 2018
Accepted:
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| Fund: This work was supported by the Key Foundation for Basic and Application Research in Higher Education of Guangdong, China (2017KZDXM009), the China Postdoctoral Science Foundation (2018M640789) and the Provincial Agricultural Science Innovation and Promotion Project, China. (2018LM2150). |
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Corresponding Authors:
Correspondence WANG Chong, Tel: +86-20-85285031, Fax: +86-20-85280740, E-mail: betty@scau.edu.cn
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| About author: Hong Quyen Dang, Tel: +84-983816582, E-mail: quyendangbafu @gmail.com; |
Cite this article:
Hong Quyen Dang, XU Gu-li, HOU Lian-jie, XU Jian, HONG Guang-liang, Chingyuan Hu, WANG Chong.
2020.
MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle. Journal of Integrative Agriculture, 19(1): 225-233.
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