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Journal of Integrative Agriculture  2019, Vol. 18 Issue (10): 2351-2360    DOI: 10.1016/S2095-3119(19)62735-8
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification of SNPs and expression patterns of FZD3 gene and its effect on wool traits in Chinese Merino sheep (Xinjiang Type)
ZHAO Bing-ru1, 2, FU Xue-feng2, TIAN Ke-chuan2, HUANG Xi-xia1, DI Jiang2, BAI Yan2, XU Xin-ming2, TIAN Yue-zhen2, WU Wei-wei2, ABLAT Sulayman1, ZENG Wei-dan1, HANIKEZI Tulafu2
1 College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, P.R.China
2 Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi 830011, P.R.China
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As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes.  However, its effects on wool traits are not clear.  The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not.  PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs.  Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels.  The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively.  Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD) (P=0.04) and live weight (LW) (P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW) (P=0.04).  The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group.  Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes.  While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos.  Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).
Keywords:  Chinese Merino sheep (Xinjiang type)        FZD3         single nucleotide polymorphism (SNP)        expression pattern        association analysis        wool traits  
Received: 29 July 2018   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31360543 and 31760655), the earmarked fund for the China Agriculture Research System (CARS-39), the Open Project of Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, China (2016D03017) and the Postdoctoral Science Foundation, China (2017M623287). 
Corresponding Authors:  Correspondence TIAN Ke-chuan, E-mail:; HUANG Xi-xia, E-mail:   
About author:  ZHAO Bing-ru, E-mail:;

Cite this article: 

ZHAO Bing-ru, FU Xue-feng, TIAN Ke-chuan, HUANG Xi-xia, DI Jiang, BAI Yan, XU Xin-ming, TIAN Yue-zhen, WU Wei-wei, ABLAT Sulayman, ZENG Wei-dan, HANIKEZI Tulafu. 2019. Identification of SNPs and expression patterns of FZD3 gene and its effect on wool traits in Chinese Merino sheep (Xinjiang Type). Journal of Integrative Agriculture, 18(10): 2351-2360.

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