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Journal of Integrative Agriculture  2019, Vol. 18 Issue (3): 644-655    DOI: 10.1016/S2095-3119(18)62001-5
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Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916
XIAO Ya-jing1, 2*, GAO Tan-tan2*, PENG Qi2, ZHANG Jie2, SUN Dong-mei1, SONG Fu-ping2 
1 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R.China
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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The methylerythritol phosphate pathway is responsible for the biosynthesis of terpenoids, the largest class of secondary metabolites.  Although the structures and functions of the proteins involved in this pathway have been well studied in Bacillus subtilis, only a few studies have reported the transcriptional profile of the genes involved.  Therefore, we analyzed methylerythritol phosphate pathway genes in the genome of B. subtilis 916, which has been developed as a biological control agent against some rice diseases in China.  Our results showed that methylerythritol phosphate pathway genes were distributed throughout the genome of this strain.  These genes were transcribed during both the exponential and stationary phases.  We further confirmed the transcription units of dxs, dxr, ispD, ispF, ipK, ispG, ispH, idi, and ispA in B. subtilis 916 through reverse transcription-PCR analyses; the results showed that these nine genes were located in seven different operons.  The transcript start sites of the seven different operons were determined by 5´-rapid amplification of cDNA ends-PCR.  Thus, our study provides a molecular basis at the transcriptional level for investigating homoterpene synthesis in the methylerythritol phosphate pathway of B. subtilis 916.
Keywords:  methylerythritol phosphate pathway        transcription        Bacillus subtilis        biological control  
Received: 21 December 2017   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31530095).
Corresponding Authors:  Correspondence SUN Dong-mei, Mobile: +86-13089069957, E-mail:; SONG Fu-ping, Tel: +86-10-62896634, E-mail:   
About author:  XIAO Ya-jing, Tel: +86-10-62815923, E-mail:; GAO Tan-tan, Tel: +86-10-62815923, E-mail:; * These authors contributed equally to this study.

Cite this article: 

XIAO Ya-jing, GAO Tan-tan, PENG Qi, ZHANG Jie, SUN Dong-mei, SONG Fu-ping. 2019. Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916. Journal of Integrative Agriculture, 18(3): 644-655.

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