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| DNA methylation patterns of banana leaves in response to Fusarium oxysporum f. sp. cubense tropical race 4 |
| LUO Jing-yao, PAN Xiao-lei, PENG Tie-cheng, CHEN Yun-yun, ZHAO Hui, MU Lei, PENG Yun, HE Rui, TANG Hua |
| Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou 570228, P.R.China |
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Abstract Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this study, with methylation-sensitive amplification polymorphism (MSAP) technique, DNA methylation was compared between the leaves inoculated with Foc TR4 and the mock-inoculated leaves at different pathogenic stages. With 25 pairs of primers, 1 144 and 1 255 fragments were amplified from the infected and mock-inoculated leaves, respectively. DNA methylation was both changed and the average methylated CCGG sequences were 34.81 and 29.26% for the infected and the mock-inoculated leaves. And DNA hypermethylation and hypomethylation were induced by pathogen infection during all pathogenic stages. Further, 69 polymorphic fragments were sequenced and 29 of them showed sequence similarity to genes with known functions. And RT-PCR results of four genes indicated that their expression patterns were consistent with their methylation patterns. Our results suggest that DNA methylation plays important roles in pathogenic response to Foc TR4 for banana.
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Received: 05 June 2016
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (30860149 and 31360364), the Joint Support Program from Tropical Crop Breeding Engineering Center of Ministry of Education of China and the Crop Science National Key Disciplines of China (lhxm-2012-2), and the Key Scientific Research Program from Hainan Province, China (ZDZX2013023). |
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Corresponding Authors:
TANG Hua, Mobile: +86-13138915025, E-mail: thtiger@163.com
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| About author: LUO Jing-yao, E-mail: sunney729@163.com |
Cite this article:
LUO Jing-yao, PAN Xiao-lei, PENG Tie-cheng, CHEN Yun-yun, ZHAO Hui, MU Lei, PENG Yun, HE Rui, TANG Hua.
2016.
DNA methylation patterns of banana leaves in response to Fusarium oxysporum f. sp. cubense tropical race 4. Journal of Integrative Agriculture, 15(12): 2736-2744.
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| De Ascensao A R, Dubery I A. 2000. Panama disease: cell Wall reinforcement in banana roots in response to elicitors from Fusarium oxysporum f. sp. cubense race four. Phytopathology, 90, 1173–1180.Ayyadurai N, Ravindra Naik P, Sreehari Rao M, Sunish Kumar R, Samrat S K, Manohar M, Sakthivel N. 2006. Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana. Journal of Applied Microbiology, 100, 926–937.Bender J. 2004. DNA methylation and epigenetics. Annual Review of Plant Biology, 55, 41–68.Boyko A, Kathiria P, Zemp F J, Yao Y, Pogribny I, Kovalchuk I. 2007. Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability). Nucleic Acids Research, 35, 1714–1725.Chinnusamy V, Zhu J K. 2009. RNA-directed DNA methylation and demethylation in plants. Science China Life Sciences, 52, 331–343.Chisholm S T, Coaker G, Day B, Staskawicz B J. 2006. Host-microbe interactions: shaping the evolution of the plant immune response. Cell, 124, 803–814.D’Hont A, Denoeud F, Aury J M, Baurens F C, Carreel F, Garsmeur O, Noel B, Bocs S, Droc G, Rouard M, Da Silva C, Jabbari K, Cardi C, Poulain J, Souquet M, Labadie K, Jourda C, Lengelle J, Rodier-Goud M, Alberti A, et al. 2012. The banana (Musa acuminata) genome and the evolution of monocotyledonous plants. Nature, 488, 213–217.Dowen R H, Pelizzola M, Schmitz R J, Lister R, Dowen J M, Nery J R, Dixon J E, Ecker J R. 2012. Widespread dynamic DNA methylation in response to biotic stress. Proceedings of the National Academy of Sciences of the United States of America, 109, e2183–2191.Guo L, Han L, Yang L, Zeng H, Fan D, Zhu Y, Feng Y, Wang G, Peng C, Jiang X, Zhou D, Ni P, Liang C, Liu L, Wang J, Mao C, Fang X, Peng M, Huang J. 2014. Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. PLOS One, 9, e95543.Gurjar G, Barve M, Giri A, Gupta V. 2009. Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia, 101, 484–495.Joobeur T, King J J, Nolin S J, Thomas C E, Dean R A. 2004. The Fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. The Plant Journal, 39, 283–297.Jordon-Thaden I E, Chanderbali A S, Gitzendanner M A, Soltis D E. 2015. Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta. Applications in Plant Sciences, 3, 1400105. Li C, Shao J, Wang Y, Li W, Guo D, Yan B, Xia Y, Peng M. 2013. Analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of Fusarium oxysporum f. sp. cubense. BMC Genomics, 14, 851.Li J, Gao F, Li N, Li S, Yin G, Tian G, Jia S, Wang K, Zhang X, Yang H, Nielsen A L, Bolund L. 2009. An improved method for genome wide DNA methylation profiling correlated to transcription and genomic instability in two breast cancer cell lines. BMC Genomics, 10, 223.Manfioletti G, Schneider C. 1998. A new and fast method for preparing high quality lambda DNA suitable for sequencing. Nucleic Acids Research, 16, 2873-2884.Peraza-Echeverria S, Herrera-Valencia V A, Kay A. 2001. Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP). Plant Science, 161, 359–367.Peraza-Echeverria S, James-Kay A, Canto-Canche B, Castillo-Castro E. 2007. Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in banana. Molecular Genetics and Genomics, 278, 443–453.Ploetz R C. 2006. Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology, 96, 653–656.Sha A H, Lin X H, Huang J B, Zhang D P. 2005. Analysis of DNA methylation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP (MSAP) analysis. Molecular Genetics and Genomics, 273, 484–490.Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P. 1998. Dissection of the fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. The Plant Cell, 10, 1055–1068.Tang X M, Tao X, Wang Y, Ma D W, Li D, Yang H, Ma X R. 2014. Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique. Molecular Genetics and Genomics, 289, 1075–1084.Vanyushin B F, Ashapkin V V. 2011. DNA methylation in higher plants: past, present and future. Molecular Genetics and Genomics, 1809, 360–368.Wang P, Xia H, Zhang Y, Zhao S, Zhao C, Hou L, Li C, Li A, Ma C, Wang X. 2015. Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in maize (Zea may). BMC Genomics, 16, 21.Wang Z, Zhang J, Jia C, Liu J, Li Y, Yin X, Xu B, Jin Z. 2012. De novo characterization of the banana root transcriptome and analysis of gene expression under Fusarium oxysporum f. sp. cubense tropical race 4 infection. BMC Genomics, 13, 650.Xie J, Zhang X Y, Chen Y H, Chen C Y, Wei S S, Xia Y Q, Tang H. Isolation of Fusarium oxysporum f. sp. cubense and its morphological investigation. 2010. Journal of Tropical Organisms, 1, 337–341. (in Chinese)Xiong L Z, Xu C G, Saghai Maroof M A, Zhang Q. 1999. Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique. Molecular Genetics and Genomics, 261, 439–446.Xu Y, Zhong L, Wu X, Fang X, Wang J. 2009. Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Planta, 229, 471–483.Zhao M L, Wang J N, Shan W, Fan J G, Kuang J F, Wu K Q, Li X P, Chen W X, He F Y, Chen J Y and Lu W J. 2013. Induction of jasmonate signalling regulators MaMYC2s and their physical interactions with MaICE1 in methyl jasmonate-induced chilling tolerance in banana fruit. Plant, Cell & Environment, 36, 30–51.Zhang X, Yazaki J, Sundaresan A, Cokus S, Chan S W, Chen H, Henderson I R, Shinn P, Pellegrini M, Jacobsen S E, Ecker J R. 2006. Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell, 126, 1189–1201. |
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