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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 1033-1043    DOI: 10.1016/S2095-3119(20)63157-4
Special Issue: Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
The MADS-box transcription factor CmAGL11 modulates somatic embryogenesis in Chinese chestnut (Castanea mollissima Blume)
GAO Yue-rong1, 2*, SUN Jia-chen1*, SUN Zhi-lin1, XING Yu1, ZHANG Qing3, FANG Ke-feng4, CAO Qing-qin1, QIN Ling1 
1 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, P.R.China
2 Beijing Bei Nong Enterprise Management Co., Ltd., Beijing 102206, P.R.China
3 College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, P.R.China
4 Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture, Beijing 102206, P.R.China
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Abstract  Somatic embryogenesis (SE) is an effective approach of in vitro regeneration that depends on plant cell totipotency. However, largely unknown of molecular mechanisms of SE in woody plants such as Chinese chestnut (Castanea mollissima Blume), limits the development of the woody plant industry. Here, we report the MADS-box transcription factor CmAGL11 in Chinese chestnut. CmAGL11 transcripts specifically accumulated in the globular embryo. Overexpression of CmAGL11 in chestnut callus enhanced its SE capacity, and the development of somatic embryos occurred significantly faster than in the control. RNA-seq results showed that CmAGL11 affects the expression of several genes related to the gibberellin, auxin, and ethylene pathways. Moreover, the analysis of DNA methylation status indicated that the promoter methylation plays a role in regulation of CmAGL11 expression during SE. Our results demonstrated that CmAGL11 plays an important role in the SE process in Chinese chestnut, possibly by regulating gibberellin, auxin, and ethylene pathways. It will help establish an efficient platform to accelerate genetic improvement and germplasm innovation in Chinese chestnut.
Keywords:  Chinese chestnut        CmAGL11        somatic embryogenesis        DNA methylation  
Received: 02 December 2019   Accepted: 04 March 2020
Fund: This work was supported by the National Key Research & Development Program of China (2018YFD1000605), the National Natural Science Foundation of China (31870671 and 31672135), and the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality, China (IDHT20180509) and the Supporting Plan for Cultivating High Level Teachers in Colleges and Universities in Beijing, China (CIT&TCD20180317).
Corresponding Authors:  Correspondence CAO Qing-qin, Tel: +86-10-80797229, E-mail: caoqingqin@bua.edu.cn; QIN Ling, Tel: +86-10-80797229, E-mail: qinlingbac@126.com   
About author:  * These authors contributed equally to this study.

Cite this article: 

GAO Yue-rong, SUN Jia-chen, SUN Zhi-lin, XING Yu, ZHANG Qing, FANG Ke-feng, CAO Qing-qin, QIN Ling. 2020. The MADS-box transcription factor CmAGL11 modulates somatic embryogenesis in Chinese chestnut (Castanea mollissima Blume). Journal of Integrative Agriculture, 19(4): 1033-1043.

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