Please wait a minute...
Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2369-2379    DOI: 10.1016/S2095-3119(15)61272-2
Soil & Fertilization﹒Irrigation﹒Plant Nutrition﹒ Agro-Ecology & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Screening of a microbial consortium with efficient corn stover degradation ability at low temperature
Qinggeer1, GAO Ju-lin1, YU Xiao-fang1, ZHANG Bao-lin2, WANG Zhi-gang1, Borjigin Naoganchaolu1, HU Shu-ping1, SUN Ji-ying1, XIE Min1, WANG Zhen1
1 Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R.China
2 Chemistry and Environmental Science College, Inner Mongolia Normal University, Hohhot 010019, P.R.China
Export:  BibTeX | EndNote (RIS)      
Abstract      To speed up the degradation of corn stover directly returned to soil at low temperature, the corn stover-degrading microbial consortium GF-20, acclimated to biological decomposition in the frigid region, was successfully constructed under a long-term limiting substrate. To evaluate its potential in accelerating the decomposition of un-pretreated corn stover, the decomposing property, fermentation dynamic and the microbial diversity were analyzed. GF-20 degraded corn stover by 32% after 15-day fermentation at 10°C. Peak activities of filter paperlyase (FPA), β-glucosidases (CB), endoglucanases (Cx), and cellobiohydrolases (C1) were 1.15, 1.67, 1.73, and 1.42 U mL–1, appearing at the 6th, 3rd, 11th, and 9th d, respectively. The pH averaged at 6.73–8.42, and the optical density (OD) value peaked at 1.87 at the 120 h of the degradation process. Cellulase, hemicellulase and lignin in corn stover were persistently degraded by 44.85, 43.85 and 25.29% at the end of incubation. Result of denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that GF-20 had a stable component structure under switching the temperature and pH. The composition of the GF-20 was also analyzed by constructing bacterial 16S rDNA clone library and fungal 18SrDNA-PCR-DGGE. Twenty-two bacterial clones and four fungal bands were detected and identified dominant bacteria represented by Cellvibrio mixtus subsp., Azospira oryzae, Arcobacter defluyii, and Clostridium populeti and the fungi were mainly identified as related to Trichosporon sp.
Keywords:  corn stover        degradation        microbial consortium        low temperature  
Received: 24 July 2015   Accepted:

  This study was supported by the National Natural Science Foundation of China (31260300), the National Maize Industrial Technology Systems, China (CARS-02-63), the Science & Technology Project for Food Production, China (2011BAD16B13, 2012BAD04B04, 2013BAD07B04), the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120).

Corresponding Authors:  GAO Ju-lin, Tel: +86-471-4301317, E-mail:   

Cite this article: 

Qinggeer , GAO Ju-lin, YU Xiao-fang, ZHANG Bao-lin, WANG Zhi-gang, Borjigin Naoganchaolu, HU Shu-ping, SUN Ji-ying, XIE Min, WANG Zhen. 2016. Screening of a microbial consortium with efficient corn stover degradation ability at low temperature. Journal of Integrative Agriculture, 15(10): 2369-2379.

Anna J S, ltita S, Brigitta B, Balazs V, Csaba H, Karoly M. 2009. DGGE and T-RFLP analysis of bacterial succession during mushroom compost production and sequence aided T-RFLP profile of mature compost. Microbial Ecology, 57, 522–533.

An S Y, Asahara M, Goto K, Kasai H, Yokota A. 2007. Terribacillus saccharophilus gen. nov., sp. nov. and Terribacillus halophilus sp. nov., spore-forming bacteria isolated from field soil in Japan. International Journal of Systematic and Evolutionary Microbiology, 57, 51–55.

Avrahami S, Liesack W, Conrad R. 2003. Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers. Environmental Microbiology, 5, 691–705.

Bi Y Y, Gao C Y, Wang Y J. 2009. Estimation of straw resources in China. Transaction of the Chinese Society of Agricultural Engineering, 12, 211–217.

Chun J, Lee J H, Jung Y. 2007. EzTaxon: A web- based tool for identification of prokaryotes based on 16S ribosomal RNA gens sequences. International Journal of Systematic and Evolutionary Microbiology, 57, 2259–2261.

Cui Z J, Li M D, Piao Z, Yong H Z, Ishii M, Igarashi Y. 2002. Selection of a composite microbial system MC1 with efficient and stability cellulose degradation bacteria and its function. Environmental Science, 23, 36–39.

Freier D, Mothershed C P, Wiegel J. 1988. Characterization of Clostridium thermocellum JW20. Applied and Environmental Microbiology, 54, 204–211.

Gao L J, Yang H Y, Wang X F, Huang Z Y, Ishiic M, Igarashic Y, Cui Z J. 2008. Rice straw fermentation using lactic acid bacteria. Bioresource Technology, 99, 2742–2748.

Garbeva P, Veen J A, Elsas J D. 2004. Microbial diversity in soil: Selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 42, 243–270.

Guerfali M, Saidi A, Gargouri A, Belghith H. 2015. Enhanced enzymatic hydrolysis of waste paper for ethanol production using separate saccharification and fermentation. Applied Biochemistry and Biotechnology, 175, 25–42.

Guo P, Wang X F, Zhu W B, Yang H Y, Cheng X, Cui Z J. 2008. Degradation of corn stover by the composite microbial system of MC1. Journal of Environmental Sciences, 20, 109–114.

Haruta S, Cui Z J, Huang Z Y. 2002. Construction of a stable microbial community with high cellulose degradation ability. Applied Microbiology and Biotechnology, 59, 529–534.

Honeina K, Kanekoa G, Katsuyamab I, Matsumotob M, Kawashimab Y, Yamadac M, Watabea S. 2012. Studies on the cellulose-degrading system in a shipworm and its potential applications. Energy Procedia, 18, 1271–1274.

Juhanson J, Truu J, Heinaru E, Heinaru A. 2009. Survival and catabolic performance of introduced Pseudomonas strains during phytoremediationand bioaugmentation experiment. FEMS Microbiology Ecology, 70, 446–455.

Kirclman D L. 2002. The ecology of cytphagic-flavobacteria in aquat is enviroanents. FEMS Microbiology Ecology, 39, 91–100.

Lane D J. 1991. 16S/23S rRNA sequencing. In: Stackebrandt E, eds., Nucleic Acids Techniques in Bacterial Systematics. John Wiley, Sons, Chichester, UK. pp. 115-175.

Liang X, Fu Y, Tong L, Liu H. 2014. Microbial shifts of the silkworm larval gut in response to lettuce leaf feeding. Applied Microbiology and Biotechnology, 98, 3769–3776.

Li J, Liu G, Chen M, Li Z, Qin Y, Qu Y. 2013. Cellodextrin transporters play important roles in cellulase induction in the cellulolytic fungus Penicillium oxalicum. Applied Microbiology and Biotechnology, 97, 10479–10488.

Li L, Li K, Wang K, Chen C, Gao C, Ma C, Xu P. 2014. Efficient production of 2,3-butanediol from corn stover hydrolysate by using a thermophilic Bacillus licheniformis strain. Bioresource Technology, 170, 256–261.

Li P P, Wang X J, Yuan X F, Wang X F, Cao Y Z, Cui Z J. 2011. Screening of a composite microbial system and its characteristics of wheat straw degradation. Agricultural Sciences in China, 10, 1586–1594.

Liu C L, Wang X F, Wang X J, Li P P, Cui Z J. 2010. The character of normal temperature straw-rotting microbial community. Agricultural Sciences in China, 9, 101–105.

Lü Y C, Li N, Gong D H, Wang X F, Cui Z J. 2012. The effect of temperature on the structure and function of a cellulose-degrading microbial community. Applied Biochemistry and Biotechnology, 168, 219–233.

Lü Y C, Wang X F, Li N, Wang X J, Ishii M, Igarashi Y, Cui Z J. 2011. Characterization of the effective cellulose degrading strain CTL-6. Journal of Environmental Sciences, 23, 649–655.

Lv K Y, Chou H G, Bai J F. 2013. Development of direct return of corn stover to soil: Current status, driving forces and constraints. China Population Resource and Environment, 23, 171–176.

Maki M L, Broere M, Leung K T, Qin W. 2011. Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. International Journal of Biochemistry and Molecular Biology, 2, 146–154.

Okeke B C. 2014. Cellulolytic and xylanolytic potential of high β-glucosidase-producing Trichoderma from decaying biomass. Applied Biochemistry and Biotechnology, 174, 1581–1598.

Panswad T, Doungchai A, Anotai J. 2003. Temperature effect on microbial community of enhanced biological phosphorus removal system. Water Research, 37, 409–415.

Polz M F, Cavanaugh C M. 1998. Bias in template to product ratios in multitemplate PCR. Applied and Environmental Microbiology, 64, 3724–3730.

Ren Z, Ward T E, Logan B E, Regan J M. 2007. Characterization of the cellulolytic and hydrogen-producing activities of six mesophilic Clostridium species. Journal of Applied Microbiology, 103, 2258–2266.

Rogers G M, Baecker A A W. 1991. Clostridium xylanolyticum sp. nov., an anaerobic xylanolytic bacterium from decayed Pinus patula wood chips. International Journal of Systematic and Evolutionary Microbiology, 41, 140–143.

Sarula, Gao J L, Yu X F, Hu S P. 2013. Screening of low temperature maize stover decomposition microorganism. Agricultural Sciences in China, 46, 4082–4090.

Strakowska J, B?aszczyk L, Che?kowski J. 2014. The significance of cellulolytic enzymes produced by Trichoderma in opportunistic lifestyle of this fungus. Journal of Basic Microbiology, 54, 2–13.

Sukumaran R K, Singhania R R, Mathew G, Pandey A. 2009. Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bioethanol production. Renewable Energy, 34, 421–424.

Ulrich A, Gabriele K, Stephan W. 2008. Diversity and activity of cellulose-decomposing bacteria, isolated from a soamy soil after long-term manure application. FEMS Microbiology Ecology, 55, 512–522.

Vinas M, Sabate J, Guasp C, Lalucat, Solanas A M. 2005. Culture dependent and independent approaches establish the complexity of a PAH degrading microbial consortium. Canadian Journal of Microbiology, 51, 897.

Wang H, Li J J, Lü Y C, Guo P, Wang X F, Kazuhirod M, Cui Z J. 2013. Bioconversion of un-pretreated lignocellulosic materials by a microbial consortium XDC-2. Bioresource Technology, 136, 481–487.

Wang R F, Zhang J W, Dong S T, Liu P. 2011. Present situation of maize straw resource utilization and its effect in main maize production regions of China. Chinese Journal of Applied Ecology, 22, 1504–1510. (in Chinese)

White T J, Bruns T D, Lee S. 1990. Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. PCR Protocols: A Guide to Methods and Applications. USA. pp. 315–322.

Wu X Y, Zheng G, Zhang W W, Xu X W, Wu M, Zhu X F. 2010. Amphibacillus jilinensis sp. Nov., a facultatively anaerobic and alkaliphilic bacilli from a soda lake in China. International Journal of Systematic and Evolutionary Microbiology, 60, 2540–2543.

Wu Y R, He J. 2015. Characterization of a xylanase producing Cellvibrio mixtus strain J3-8 and its genome analysis. Scientific Reports, 21, 10521.

Zhang D D, Yuan X F, Guo P, Suo Y L, Wang X F, Wang W D, Cui Z J. 2011. Microbial population dynamics and changes in main nutrients during the acidification process of pig manures. Journal of Environmental Sciences, 23, 497–505.

Zhang Q Z, Cai W M, Wang J. 2008. Stimulatory effects of biosurfactant produced by Pseudomonas aeruginosa BSZ-07 on rice straw decomposing. Journal of Environmental Sciences, 20, 975–980.

Zhu H, Qu F, Zhu L H. 1993. Isolation of genomic DNAs fromplants, fungi and bacteria using benzyl chloride. Nucleic Acids Research, 21, 5279–5280.
[1] YANG Ya-jun, XU Hong-xing, WU Zhi-hong, LU Zhong-xian. Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2195-2203.
[2] FENG Chang-qing, CHENG Deng-miao, FENG Yao, QI Wei-ning, JIA Zhen-hu, Louise WEAVER, LIU Yuan-wang, LI Zhao-jun. Screening and degradation characteristics of a tylosin-degrading strain[J]. >Journal of Integrative Agriculture, 2020, 19(4): 1127-1136.
[3] YANG Ya-jun, XU Hong-xing, WU Zhi-hong, LU Zhong-xian. pH influences the profiles of midgut extracts in Cnaphalocrocis medinalis (Guenée) and its degradation of activated Cry toxins[J]. >Journal of Integrative Agriculture, 2020, 19(3): 775-784.
[4] GUO Gang, SHEN Chen, LIU Qiang, ZHANG Shuan-lin, SHAO Tao, WANG Cong, WANG Yong-xin, XU Qing-fang, HUO Wen-jie.
The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage
[J]. >Journal of Integrative Agriculture, 2020, 19(3): 838-847.
[5] LI Zhao-jun, QI Wei-ning, FENG Yao, LIU Yuan-wang, Ebrahim Shehata, LONG Jian . Degradation mechanisms of oxytetracycline in the environment[J]. >Journal of Integrative Agriculture, 2019, 18(9): 1953-1960.
[6] YU Xiao-fang, BORJIGIN Qinggeer, GAO Ju-lin, WANG Zhi-gang, HU Shu-ping, BORJIGIN Naoganchaolu, WANG Zhen, SUN Ji-ying, HAN Sheng-cai.
Exploration of the key microbes and composition stability of microbial consortium GF-20 with efficiently decomposes corn stover at low temperatures
[J]. >Journal of Integrative Agriculture, 2019, 18(8): 1893-1904.
[7] TIAN Xing-zhou, Pramote Paengkoum, Siwaporn Paengkoum, Sorasak Thongpea, BAN Chao. Comparison of forage yield, silage fermentative quality, anthocyanin stability, antioxidant activity, and in vitro rumen fermentation of anthocyanin-rich purple corn (Zea mays L.) stover and sticky corn stover[J]. >Journal of Integrative Agriculture, 2018, 17(09): 2082-2095.
[8] PAN Xiao-hua, YANG Liang, Yves Beckers, XIONG Ben-hai, JIANG Lin-shu. Accuracy comparison of dry matter intake prediction models evaluated by a feeding trial of lactating dairy cows fed two total mixed rations with different forage source[J]. >Journal of Integrative Agriculture, 2017, 16(04): 921-929.
[9] QIN Meng-zhen , SHEN Yi-xin. Effect of Application of a Bacteria Inoculant and Wheat Bran on Fermentation Quality of Peanut Vine Ensiled Alone or with Corn Stover[J]. >Journal of Integrative Agriculture, 2013, 12(3): 556-560.
No Suggested Reading articles found!