|Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
|YANG Ya-jun, XU Hong-xing, WU Zhi-hong, LU Zhong-xian
|State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
Midgut juice plays an important role in food digestion and detoxification in insects. In order to understand the potential of midgut juice of Cnaphalocrocis medinalis (Guenée) to degrade Bt proteins, the enzymatic activity of midgut juice and its degradation of Bt proteins (Cry2A, Cry1C, Cry1Aa, and Cry1Ac) were evaluated in this study through protease inhibitor treatments. The activities of total protease in midgut juices were significantly inhibited by phenylmethylsulfonyl fluoride (PMSF), tosyl-L-lysine chloromethyl ketone (TLCK), pepstatin A and leupeptin. The enzymatic activity of chymotrypsin was significantly inhibited by PMSF, and enzymatic activity of trypsin was significantly inhibited by ethylenediaminetetraacetic acid (EDTA), PMSF, tosyl phenylalanine chloromethyl ketone (TPCK), TLCK and trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E-64). EDTA could significantly inhibit the degradation of Cry2A by C. medinalis. EDTA, PMSF, TPCK, and TLCK could inhibit the degradation of Cry1C and Cry1Aa. EDTA, PMSF, TPCK, TLCK, and E-64 could inhibit the degradation of Cry1Ac. Our results indicated that some protease inhibitors hindered various enzymatic activities in the larval midgut of C. medinalis, which may reduce the insect’s ability to degrade Bt toxins. These findings may aid the application of protease inhibitors in the management of this insect pest in the future.
Received: 02 April 2020
|Fund: This study was supported by the Zhejiang Provincial Natural Science Foundation of China (LY20C140004), the National Science and Technology Major Project of China (2016ZX08001001), the National Natural Science Foundation of China (31501669) and the earmarked fund for China Agriculture Research System (CARS-01-36).
Correspondence LU Zhong-xian, E-mail: email@example.com
|About author: YANG Ya-jun, E-mail: firstname.lastname@example.org;
Cite this article:
YANG Ya-jun, XU Hong-xing, WU Zhi-hong, LU Zhong-xian.
Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae). Journal of Integrative Agriculture, 20(8): 2195-2203.
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