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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 1127-1136    DOI: 10.1016/S2095-3119(19)62764-4
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Screening and degradation characteristics of a tylosin-degrading strain
FENG Chang-qing1, 2, CHENG Deng-miao2, FENG Yao2, QI Wei-ning2, JIA Zhen-hu1, Louise WEAVER3, LIU Yuan-wang2, 3, LI Zhao-jun 
1 College of Life Science, Shanxi Normal University, Linfen 041004, P.R.China
2 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3 Institute of Environmental and Science Research Ltd., Christchurch Science Centre, Christchurch 8041, New Zealand
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Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.  Recalcitrant antibiotic residues, such as tylosin (TYL), can cause serious environmental problems, which makes it important to eliminate TYL from the environment.  It is important to eliminate TYL from the environment.  In this study, a strain was isolated and purified from fermentation by-product that came from a TYL production factory.  The TYL degrading strain was identified by its morphology, physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16S rDNA-coding genes.  The temperature, shaking speed, initial TYL concentration, pH and inoculum sizes were investigated under simulated conditions by using single factor tests.  The results showed that TYL2, a high efficient strain was isolated and was identified as Brevibacillus borstelensis.  The degradation rate of TYL by this strain could reach to 75% with an initial concentration of 25 mg L–1 within 7 days under conditions of 7% B. borstelensis (v/v, 2×108 CFU mL–1) at pH 7.0 and at 35°C.  It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65% within 7 days.  This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.
Keywords:  antibiotics        tylosin        screening        degrading strain        B. borstelensis        degradation characteristic  
Received: 12 April 2019   Accepted: 04 March 2020
Fund: This work was jointly supported by the National Key R&D Program of China (2018YFD0500206), the National Natural Science Foundation of China (31772395), and the Fundamental Research Funds for Central Non-profit Scientific Institution, Chinese Academy of Agricultural Sciences (1610132019046).
Corresponding Authors:  Correspondence LI Zhao-jun, Tel: +86-10-82108657, E-mail:; JIA Zhen-hu, E-mail:   

Cite this article: 

FENG Chang-qing, CHENG Deng-miao, FENG Yao, QI Wei-ning, JIA Zhen-hu, Louise WEAVER, LIU Yuan-wang, LI Zhao-jun. 2020. Screening and degradation characteristics of a tylosin-degrading strain. Journal of Integrative Agriculture, 19(4): 1127-1136.

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