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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 1127-1136    DOI: 10.1016/S2095-3119(19)62764-4
Special Issue: Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Screening and degradation characteristics of a tylosin-degrading strain
FENG Chang-qing1, 2, CHENG Deng-miao2, FENG Yao2, QI Wei-ning2, JIA Zhen-hu1, Louise WEAVER3, LIU Yuan-wang2, 3, LI Zhao-jun 
1 College of Life Science, Shanxi Normal University, Linfen 041004, P.R.China
2 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3 Institute of Environmental and Science Research Ltd., Christchurch Science Centre, Christchurch 8041, New Zealand
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Abstract  
Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.  Recalcitrant antibiotic residues, such as tylosin (TYL), can cause serious environmental problems, which makes it important to eliminate TYL from the environment.  It is important to eliminate TYL from the environment.  In this study, a strain was isolated and purified from fermentation by-product that came from a TYL production factory.  The TYL degrading strain was identified by its morphology, physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16S rDNA-coding genes.  The temperature, shaking speed, initial TYL concentration, pH and inoculum sizes were investigated under simulated conditions by using single factor tests.  The results showed that TYL2, a high efficient strain was isolated and was identified as Brevibacillus borstelensis.  The degradation rate of TYL by this strain could reach to 75% with an initial concentration of 25 mg L–1 within 7 days under conditions of 7% B. borstelensis (v/v, 2×108 CFU mL–1) at pH 7.0 and at 35°C.  It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65% within 7 days.  This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.
Keywords:  antibiotics        tylosin        screening        degrading strain        B. borstelensis        degradation characteristic  
Received: 12 April 2019   Accepted: 04 March 2020
Fund: This work was jointly supported by the National Key R&D Program of China (2018YFD0500206), the National Natural Science Foundation of China (31772395), and the Fundamental Research Funds for Central Non-profit Scientific Institution, Chinese Academy of Agricultural Sciences (1610132019046).
Corresponding Authors:  Correspondence LI Zhao-jun, Tel: +86-10-82108657, E-mail: lizhaojun@caas.cn; JIA Zhen-hu, E-mail: jiaxueshi@163.com   

Cite this article: 

FENG Chang-qing, CHENG Deng-miao, FENG Yao, QI Wei-ning, JIA Zhen-hu, Louise WEAVER, LIU Yuan-wang, LI Zhao-jun. 2020. Screening and degradation characteristics of a tylosin-degrading strain. Journal of Integrative Agriculture, 19(4): 1127-1136.

Bird S B, Sutherland T D, Gresham C, Eddleston M. 2008. OpdA, a bacterial hydrolase, prevents lethality in rats after poisoning with highly toxic organophosphorus pesticides. Toxicology, 247, 88–92.
Carucci A, Cappai G, Piredda M. 2006. Biodegradability and toxicity of pharmaceuticals in biological wastewater treatment plants. Journal of Environmental Science and Health (Part A), 41, 1831–1842.
Chee-Sanford J C, Mackie R I, Koike S, Krapac I G, Lin Y F, Yannarell A C, Maxwell S, Aminov R I. 2009. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste. Journal of Environmental Quality, 38, 1086–1108.
Chen J, Wei X D, Liu Y S, Ying G G, Liu S S, He L Y, Su H C, Hu L X, Chen F R, Yang R Q. 2016. Removal of antibiotics and antibiotic resistance genes from domestic sewage by constructed wetlands: Optimization of wetland substrates and hydraulic loading. Science of the Total Environment, 565, 240–248.
Dolliver H, Gupta S. 2008. Antibiotic losses in leaching and surface runoff from manure-amended agricultural land. Environmental Quality, 37, 1227–1237.
Flemming I, Bent H S. 2001. Biodegradability of metronidazole, olaquindox, and TYL and formation of TYL degradation products in aerobic soil-manure slurries. Ecotoxicology and Environmental Safety, 48, 311–320.
Guo X T, Yin Y Y, Yang C, Dang Z. 2018. Maize straw decorated with sulfide for TYL removal from the water. Ecotoxicology and Environmental Safety, 152, 16–23.
He L Y, Ying G G, Liu Y S, Su H C, Chen J, Liu S S, Zhao J L. 2016. Discharge of swine wastes risks water quality and food safety: Antibiotics and antibiotic resistance genes from swine sources to the receiving environments. Environment International, 92–93, 210–219.
Hoese A, Clay S A, Clay D E, Oswald J, Trooien T, Thaler R, Carlson C G. 2009. Chlortetracyline and tylosin runoff from soils treated with antimicrobial containing manure. Journal of Environmental Science and Health (Part B: Pesticides Food Contaminants and Agricultural Wastes), 44, 371–378.
Holly D, Satish G, Sally N. 2008. Antibiotic degradation during manure composting. Journal of Environmental Quality, 37, 1245–1253.
Homem V, Santos L. 2011. Degradation and removal methods of antibiotics from aqueous matricese - a review. Journal of Environmental Management, 92, 2304–2347.
Huang M H, Tian S X, Chen D H, Zhang W, Wu J, Chen L. 2012. Removal of sulfamethazine antibiotics by aerobic sludge and an isolated Achromobacter sp. S-3. Journal of Environmental Sciences, 24, 1594–1599.
Huang X, Pan B L, Sun J Q, Zhao Y Y, Li S P. 2009. Isolation, characterization of a strain capable of degrading imazethapyr and its use in degradation of the herbicide in soil. Current Microbiology, 59, 363–367.
Jiao S, Zheng S, Yin D, Wang L H, Chen L Y. 2008. Aqueous oxytetracycline degradation and the toxicity change of degradation compounds in photoirradiation process. Journal of Environmental Sciences, 20, 806–813.
Kolz A C, Ong S K, Moorman T B. 2005. Sorption of tylosin onto swine manure. Chemosphere, 60, 284–289.
Lan S H, Wu X W, Xu P R. 2011. Study on screening of dominant bacteria for phenol degradation. International Conference on Computer Distributed Control and Intelligent Environmental Monitoring. [2019-04-06]. https://ieeexplore.ieee.org/document/5748163
Lee K, Weiset A M, St-Pierre S. 1996. Enhanced oil biodegradation with mineral fine interaction. Spill Science & Technology Bulletin, 3, 263–267.
Li B, Yang L, Wang C Q, Zhang Q P, Liu Q C, Li Y D, Xiao R. 2017. Adsorption of Cd(II) from aqueous solutions by rape straw biochar derived from different modification processes. Chemosphere, 175, 332–340.
Liu H M, Lou X, Ge Z J, Yang F, Chen D B, Zhu J C, Xu J H, Li S P, Hong Q. 2015. Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh). Brazilian Journal of Microbiology, 46, 425–432.
Liu Y W, Chang H Q, Li Z J, Zhang C, Feng Y, Cheng D M. 2016. Gentamicin removal in submerged fermentation using the novel fungal strain Aspergillus terreus FZC3. Scientific Reports, 6, 35856.
Liu Y W, Chang H Q, Li Z J, Feng Y, Cheng D M, Xue J M. 2017. Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage. Scientific Reports, 7, 11004.
Ma Y L, Wang L Q, Liu L J, Zhang X S. 2015. Biodegradation of tylosin residue in pharmaceutical solid waste by a novel Citrobacter amalonaticus strain. Environmental Progress & Sustainable Energy, 34, 99–104.
Maki T, Hasegawa H, Kitami H, Fumoto K, Munekage Y, Ueda K. 2006. Bacterial degradation of antibiotic residues in marine fish farm sediments of Uranouchi Bay and phylogenetic analysis of antibiotic-degrading bacteria using 16S rDNA sequences. Fisheries Science, 72, 811–820.
Migliore L, Fiori M, Spadoni A, Galli E. 2012. Biodegradation of oxytetracycline by Pleurotus ostreatus mycelium: A mycoremediation technique. Journal of Hazardous Materials, 215–216, 227–232.
Minkevich I G, Satroutdinov A D, Dedyukhina E G, Chistyakova T I, Kaparullina E N, Koshelev A V, Okunev O N. 2006. The effect of temperature on bacterial degradation of EDTA in pH-auxostat. World Journal of Microbiology & Biotechnology, 22, 1205–1213.
Mitchell S M, Ullman J L, Teel A L, Watts R J. 2015. Hydrolysis of amphenicol and macrolide antibiotics: Chloramphenicol, florfenicol, spiramycin, and tylosin. Chemosphere, 134, 504–511.
Mompelat S, Le Bot B, Thomas O. 2009. Occurrence and fate of pharmaceutical products and by-prodcuts, from resource to drinling water. Environmental Intermational, 35, 803–814.
Pan M, Chu L M. 2016. Adsorption and degradation of five selected antibiotics in agricultural soil. Science of the Total Environment, 545–546, 48–56.
Pawar R M. 2015. The effect of soil pH on bioremediation of polycyclic aromatic hydrocarbons (PAHS). Journal of Bioremediation & Biodegradation, 6, 291.
Porter A W, Hay A G. 2007. Identification of opdA, a gene involved in biodegradation of the endocrine disrupter octylphenol. Applied and Environmental Microbiology, 73, 7373–7379.
Prats C, Francesch R, Arboix M, Pérez B. 2001. Determination of tylosin residues in different animal tissues by hige performance liquid chromatography. Journal of Chromatography (B), 766, 57–65.
Reyes C, Fernández J, Freer J, Mondaca M A, Zaror C, Malato S, Mansilla H D. 2006. Journal of Photochemistry and Photobiology (A: Chemistry), 184, 141–146.
Rodriguez-Mozaza S, Chamorro S, Marti E, Huerta B, Gros M, Sànchez-Melsió A, Borrego C M, Barceló D, Balcázar J L. 2015. Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river. Water Research, 69, 234–242.
Saeideh K M, Mohammadhossein R, Morteza V, Mohammadreza E. 2014. Removal offrom aqueous solution by UV/nano Ag/S2 process: Influence of operational parameters and kinetic study. Korean Journal of Chemical Engineering, 31, 1577–1581.
Sanscartier D, Reimer K, Zeeb B, Koch I. 2011. The effect of temperature and aeration rate on bioremediation of diesel-contaminated soil in solid-phase bench-scale bioreactors. Soil and Sediment Contamination, 20, 353–369.
Schlusener M P, Bester K. 2006. Persistence of antibiotics such as macrolides tiamulin and salinomycin in soil. Environmental Polltuion, 143, 565–571.
Selvam A, Zhao Z, Wong J W. 2012. Composting of swine manure spiked with sulfadiazine, chlortetracycline and ciprofloxacin. Bioresource Technology, 126, 412–417.
Takayama K, Suye S, Kuroda K, Kitaguchi T, Tsuchiyama K, Fukuda T, Chen W, Mulchandni A. 2006. Surface display of organophosphorus hydrolase on Saceharomyces cerevisiae. Biotechnology Progress, 22, 939–943.
Tang J C, Taniguvhi H, Chu H, Zhou Q, Nagata S. 2008. Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes. Letters in Applied Microbiology, 48, 38–43.
Tasslit D, Laoufi A N, Bentahar F. 2011. Photocatalytic deterioration of tylosin in an aqueous suspension using UV/TiO2. Science of Advanced Materials, 3, 944–948.
Turiel E, Bordin G, Rodriguez A R. 2005. Determination of quinolones and fluoroquinolones in hospital sewage water by off-line and on-line solid-phase extraction procedures coupled to HPLC-UV. Journal of Separation Science, 28, 57–267.
Uggetti E, Sialve B, Latrille E, Steyer J P. 2014. Anaerobic digestate as substrate for microalgae culture: The role of ammonium concentration on the microalgae productivity. Bioresource Technology, 152, 437–443.
Vogna D, Marotta R, Andreozzi R, Napolitano A, Ischia M. 2004. Kinetic and chemical assessment of the UV/H2O2 treatment of antiepileptic drug carbamazepine. Chemosphere, 54, 497–505.
Wang Y, Li B F, Zhao X, Piao M Z. 2014. Isolation and characterization of a fucoidan-degrading bacterium from Laminaria japonica.  Journal of Ocean University of China (Oceanic and Coastal Sea Research), 13, 153–156.
Wu T Y, Mohammad A W, Jahim M J, Anuar N. 2009. Optimized reuse and bioconversion from retentate of pre-filtered palm oil mill effluent (POME) into microbial protease by Aspergillus terreus using response surface methodology. Journal of Chemical Technology & Biotechnology, 84, 1390–1396.
Xie Y H, Pan Y H, Bai B, Xu Z Z, Ding L, Li Q, Xu Y, Zhu T. 2015. Degradation performance and optimal parameters of two bacteria in degrading nonylphenol. Journal of Computational and Theoretical Nanoscience, 12, 2657–2663.
Yang C F, Lee C M, Wang C C. 2006. Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica. Chemosphere, 62, 709–714.
Yin F B, Dong H M, Zhang W Q, Zhu Z P, Shang B, Wang Y. 2019. Removal of combined antibiotic (florfenicol, and tilmicosin) during anaerobic digestion and their relative effect. Renewable Energy, 139, 895–903.
Yin Y Y, Guo X T, Peng D. 2018. Iron and manganese oxides modified maize straw to remove from aqueous solutions. Chemosphere, 205, 156–165.
Yu C H, Chen F, Zhao X X, Guo T, Lin R H, Qu M M, Liu Q. 2011. Isolation, identification and degrading characteristics of phenol-degrading bacteria B3. International Conference on Electrical and Control Engineering. Institute of Electrical and Electronics Engineers, New York. pp. 3509–3512.
Zhang W J, Cao J, Li K L, Sun X, Liu X L, Zhao L. 2018. Isolation and screening of tylosin-degrading microorganisms and analysis on the effect of dregs degradation. [2019-01-09]. http://www.dpi-proceedings.com/index.php/dtcse/article/download/20551/20048
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