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| Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs |
| CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu |
1、State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Key Laboratory for Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in growing pigs. A total of 24 ileal-cannulated pigs (Duroc×(Large White×Landrace) with body weight (21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet (P>0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal (linear, P<0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased (linear, P<0.05). The AID and SID of AA were not affected by the sampling time (P>0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr (P<0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal (0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.
Abstract Two experiments were conducted to determine the effects of fiber level from alfalfa meal and sampling time on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in growing pigs. A total of 24 ileal-cannulated pigs (Duroc×(Large White×Landrace) with body weight (21.4±1.5) kg) were randomly allotted to 4 treatments. The pigs were provided a corn-soybean meal diet or a diet containing 5, 10 or 20% of alfalfa meal during two 10-d experimental periods. The AID of AA was measured. Six ileal-cannulated pigs were fed a protein-free diet in order to estimate the endogenous protein losses and SID of AA. Ileal AA digestibility was not affected by inclusion of 5 or 10% alfalfa meal in the diet (P>0.05). The AID of His, Lys, Met, Phe, Thr, Val, Ala, Asp, Cys, Gly, Pro, Ser and Tyr reduced by 2.0–6.8% with the addition level of alfalfa meal (linear, P<0.05). The SID of His, Lys, Ser, Thr and Tyr decreased by 2.2–4.3% as the level of alfalfa meal in the diet increased (linear, P<0.05). The AID and SID of AA were not affected by the sampling time (P>0.05). A multiple linear regression analysis, taking into account both the soluble and insoluble fiber content in the diets, explained more than 36% of variation in SID of Ser and Thr (P<0.05). In conclusion, the corn-soybean meal diet containing 10% of alfalfa meal did not affect ileal AA digestion. The AID and SID of AA were similar between two sampling times. Increasing the concentration of total dietary fiber from 12.3 to 21.4% by adding graded levels of alfalfa meal (0–20%) to a corn-soybean meal control diet induced a linear reduction in AID and SID of most AA. Soluble and insoluble fibers from alfalfa meal have differential roles in the AA digestion, which may help explain the variation observed in the SID of partial AA. These findings would provide important information for dietary fiber level and composition related to AA digestion.
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Received: 10 October 2014
Accepted:
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| Fund: This work was financially supported by the Project of State Key Laboratory of Animal Nutrition, Ministry of Science and Technology, China (2004DA125184G1104) and the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences, China (ASTIP-IAS07). |
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Corresponding Authors:
ZHANG Hong-fu, Tel/Fax: +86-10-62818910,E-mail: zhanghfcaas@gmail.com
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| About author: CHEN Liang, E-mail: shengji0202@126.com;These authors contributed equally to this study. |
Cite this article:
CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu.
2015.
Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs. Journal of Integrative Agriculture, 14(12): 2598-2604.
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| AOAC (Association of Official Analytical Chemists). 2007.Official Methods of Analysis. 18th ed. Association of OfficialAnalytical Chemists, Gaithersburg, MD.Bach Knudsen K E. 1997. Carbohydrate and lignin contents ofplant materials used in animal feeding. Animal Feed Scienceand Technology, 67, 319-338Bach Knudsen K E, Hedemann M S, Laerke H N. 2012. Therole of carbohydrates in intestinal health of pigs. AnimalFeed Science and Technology, 173, 41-53Buraczewska L, ?wi?ch E, Tu?nio A, Taciak M, CeregrzynM, Korczyński W. 2007. The effect of pectin in amino aciddigestibility and digesta viscosity, motility and morphologyof the small intestine, and on N-balance and performanceof young pigs. Livestock Science, 109, 53-56Castillo M, Martin-Orue S M, Anguita M, Perez J F, Gasa J.2007. Adaptation of gut microbiota to corn physical structureand different types of dietary fibre. Livestock Science, 109,149-152Chen L, Gao L X, Zhang H F. 2014. Effect of graded levelsof fiber from alfalfa meal on nutrient digestibility and flowof fattening pigs. Journal of Integrative Agriculture, 13,1746-1752Chen L, Zhang H F, Gao L X, Zhao F, Lu Q P, Sa R N. 2013.Effect of graded levels of fiber from alfalfa meal on intestinalnutrient flow and hindgut fermentation of growing pigs.Journal of Animal Science, 91, 4757-4764Dégen L, Halas V, Babinszky L. 2007. Effect of dietary fibre onprotein and fat digestibility and its consequences on dietformulation for growing and fattening pigs: A review. ActaAgriculturae Scandinavica (Section A - Animal Science),57, 1-9Dilger R N, Sands J S, Ragland D, Adeola O. 2004. Digestibilityof nitrogen and amino acids in soybean meal with addedsoyhulls. Journal of Animal Science, 82, 715-724Le Goff G, van Milgen J, Noblet J. 2002. Influence of dietary fibreon digestive utilization and rate of passage in growing pigs,finishing pigs, and adult sows. Animal Science, 74, 503-515Grala W, Verstegen M W A, Jansman A J M, Huisman J,Wasilewko J. 1998. Nitrogen utilization in pigs fed diets withsoybean and rapeseed products leading to different ilealendogenous nitrogen losses. Journal of Animal Science,76, 569-577Guillon F, Saulnier L, Robert P, Thibault J F, Champ M. 2007.Chemical structure and function of cell wall through cerealgrains and vegetable samples. In: Salovaara H, GatesF, Tenkanen M, eds., Dietary Fibre Components andFunctions. Wageningen Academic Publishers, Wageningen,the Netherlands. pp. 31-64Hooda S, Metzler-Zebeli B U, Vasanthan T, Zijlstra R T. 2011.Effects of viscosity and fermentability of dietary fibre onnutrient digestibility and digesta characteristics in ilealcannulatedgrower pigs. British Journal of Nutrition, 106,664-674Hopwood D E, Pethick D W, Pluske J R, Hampson D J. 2004.Addition of pearl barley to a rice-based diet for newlyweaned piglets increases the viscosity of the intestinalcontents, reduces starch digestibility and exacerbates post-weaning colibacillosis. British Journal of Nutrition,92, 419-427Jenkins D J, Kendall C W, Axelsen M, Augustin L S, VuksanV. 2000. Viscous and nonviscous fibres, nonabsorbableand low glycaemic index carbohydrates, blood lipids andcoronary heart disease. Current Opinion in Lipidology, 11,49-56Johnson L J, Noll S, Renteria A, Shurson J. 2003. Feedingby-product high in concentration of fiber to non-ruminants.In: Third National Symposium on Alternative Feeds forLivestock and Poultry Held. Kansas City, MO, USA.Kass M L, Van Soest P J, Pond W G, Lewis B, McDowell RE. 1980. Utilization of dietary fiber from alfalfa by growingswine. I. Apparent digestibility of diet components in specificsegments of the gastrointestinal tract. Journal of AnimalScience, 50, 175-191de Lange C F M, Pluske J, Gong J, Nyachoti C M. 2010.Strategic use of feed ingredients and feed additives tostimulate gut health and development in young pigs.Livestock Science, 134, 124-134Lenis N P, Bikker P, van der Meulen J, van Diepen J T M,Bakker J G M, Jongbloed A W. 1996. Effect of dietaryneutral detergent fiber on ileal digestibility and portal fluxof nitrogen and amino acids and on nitrogen utilization ingrowing pigs. Journal of Animal Science, 74, 2687-2699Lindberg J E, Cortova Z. 1995. The effect of increasing inclusionof lucerne leaf meal in a barley-based diet on the partitionof digestion and on nutrient utilization in pigs. Animal FeedScience and Technology, 56, 11-20Lindberg J E. 2014. Fiber effects in nutrition and gut health inpigs. Journal of Animal Science and Biotechnology, 5, 15.Longland A C, Low A G, Quelch D B, Bray S P. 1993. Adaptationto the digestion of non-starch polysaccharide in growing pigsfed on cereal or semi-purified basal diets. British Journal ofNutrition, 70, 557-566Mosenthin R, Sauer W C, Ahrens F. 1994. Dietary pectin’seffect on ileal and fecal amino acid digestibility and exocrinepancreatic secretions in growing pigs. Journal of Nutrition,124, 1222-1229Noblet J, Le Goff G I. 2001. Effect of dietary fibre on theenergy value of feeds for pigs. Animal Feed Science andTechnology, 90, 35-52NRC (National Research Council, USA). 1998. NutrientRequirements of Swine. 10th ed. National Academic Press,Washington, D.C.NRC (National Research Council, USA). 2012. NutrientRequirements of Swine. 11th ed. National Academic Press,Washington, D.C.Panetta D M, Powers W J, Xin H, Kerr B J, Stadler K J. 2006.Nitrogen excretion and ammonia emissions from pigsfed modified diets. Journal of Environmental Quality, 35,1297-1308Prosky L, Asp N G, Schweizer T F, DeVires J W, Fruda I. 1988.Determination of insoluble, soluble, and total dietary fiberin foods and food products: Interlaboratory study. Journal-Association of Official Analytical Chemists, 71, 1012-1023Régnier C, Jaguelin Y, Noblet J, Renaudeau D. 2012. Ilealdigestibility of amino acids of cassava, sweet potato,cocoyam and erythrina foliages fed to growing pigs. Animal,6, 586-593SAS (Statistical Analysis System). 2009. SAS User’s Guide:Statistics. SAS Institute, Cary, NC.Schulze H, van Leeuwen P, Verstegen M W, Huisman J,Souffrant W B, Ahrens F. 1994. Effect of level of dietaryneutral detergent fiber on ileal apparent digestibility andileal nitrogen losses in pigs. Journal of Animal Science,72, 2362-2368Schürch A F, Lloyd L E, Champton E W. 1950. The use ofchromic oxide as an index for determining the digestibilityof a diet. Journal of Nutrition, 50, 629-636Souffrant W B. 2001. Effect of dietary fibre on ileal digestibilityand endogenous nitrogen losses in the pigs. Animal FeedScience and Technology, 90, 93-102Stein H H, Gibson M L, Pedersen C, Boersma M G. 2006. Aminoacid and energy digestibility in ten samples of distillers driedgrain with solubles fed to growing pigs. Journal of AnimalScience, 84, 853-860Stein H H, Seve B, Fuller M F, Moughan P J, De Lange CM G. 2007. Invited review: Amino acid bioavailabilityand digestibility in pig feed ingredients: Terminology andapplication. Journal Animal of Science, 85, 172-180Thacker P A, Haq I. 2008. Nutrient digestibility, performance andcarcass traits of growing-finishing pigs fed diets containinggraded levels of dehydrated lucerne meal. Journal of theScience of Food and Agriculture, 88, 2019-2025Urriola P E, Stein H H. 2010. Effects of distillers dried grains withsolubles on amino acid, energy, and fiber digestibility andon hindgut fermentation of dietary fiber in a corn-soybeanmeal diet fed to growing pigs. Journal of Animal Science,88, 1454-1462Wenk C. 2001. The role of dietary fibre in the digestivephysiology of the pig. Animal Feed Science and Technology,90, 21-33Wilfart A, Montagne L, Simmins P H, Noblet J, van Milgen J.2007. Effect of fibre content in the diet on the mean retentiontime in different segments of the digestive tract in growingpigs. Livestock Science, 109, 27-29Yen J T. 2004. Dehydrated alfalfa meal reduces urinary ureaexcretion in finishing gilts. Journal of Animal Science,82(suppl. 2), 68. |
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