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Journal of Integrative Agriculture
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Optimizing leaf area index estimation in mulched winter wheat: A hybrid model approach coupling PROSAIL

Chunyu Wei1, 2, Yadan Du1, 2#, Yuxuan Liu1, 2, Xiaotao Hu1, 2, Zhikai Cheng1, 2, Yang Xu1, 2, Zhengtao Zhang1, 2, Xiaobo Gu1, 2, Kadambot H.M Siddique3, Yunhui Niu4

1 Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education/Northwest A&F University, Yangling 712100, China 

2 College of Water Resources and Architectural Engineering, Northwest A&F University, Yangling 712100, China 

3 The UWA Institute of Agriculture, The University of Western Australia, M082, Perth, WA 6009, Australia 

4 Jinghui Irrigation and Drainage Management Station in Lintong District, Xi'an 712100, China


 Highlights 

Quantitative assessment of plastic mulch's impact on PROSAIL-simulated reflectance.

Development of a linear spectral hybrid model integrating PROSAIL for plastic-mulched conditions.

Optimal leaf area index (LAI) retrieval accuracy achieved through RF-LASSO coupled with enhanced PROSAIL, demonstrated model robustness for LAI retrieval in plastic-mulched fields (R²>0.7).

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摘要  

冬小麦是中国主要的粮食作物,其叶面积指数(LAI)是精准农业中用于生长评估的重要指标。基于无人机的遥感技术和PROSAIL模型已被广泛应用于LAI估算,但在覆膜条件下的估算精度因地膜的光谱干扰而受限。基于陕西省杨陵区冬小麦田间实测数据,本研究通过将实测的土壤和地膜反射率融入PROSAIL模型,构建了一种线性光谱混合模型(LSHM),以改善覆膜冬小麦的LAI反演。研究采用了无人机获取的多时相影像和田间实测LAI数据,并将该模型与随机森林(RF)和LASSO变量筛选方法相结合以提高精度。与标准PROSAIL模型相比,LSHM通过融入实测土壤和地膜光谱,显著减小了PROSAIL模拟的反射率差异,在整个生育期内平均提高了16.5%。此外,RF-LASSO优化了波段反射率(BR)和植被指数(VI)组合,增强了模型的稳定性,在抽穗期(R²=0.83RMSE=0.35MSE=0.12)和灌浆期(R²=0.71RMSE=0.50MSE=0.25)均表现出优异性能。结果表明,混合PROSAIL模型在覆膜冬小麦LAI反演估算方面具有显著潜力,为垄沟覆膜种植系统中的作物性状表型分析提供了一种稳健的方法。



Abstract  

Winter wheat is a key grain crop in China, with its leaf area index (LAI) serving as a vital indicator for growth assessment in precision agriculture. While UAV-based remote sensing and the PROSAIL model are widely used for LAI estimation, their accuracy under plastic mulch is limited due to spectral interference from mulch. Based on field measurements from winter wheat fields in Yangling, Shaanxi Province, this study developed a linear-spectral hybrid model (LSHM) by integrating measured soil and mulch reflectance into the PROSAIL model to improve LAI inversion for mulched winter wheat. UAV-collected multi-temporal images and field LAI measurements were used, and the model was coupled with random forest (RF) and LASSO variable selection to enhance accuracy. The LSHM significantly reduced reflectance differences in PROSAIL simulations by incorporating measured soil and mulch spectra, achieving R² improvements average of 16.5% across all growth stages compared to the standard PROSAIL model. Furthermore, the RF-LASSO optimized combination of band reflectance (BR) and vegetation indices (VIs) enhanced model stability, demonstrating superior performance (R²=0.83, RMSE=0.35, MSE=0.12 at heading and R²=0.71, RMSE=0.50, MSE=0.25 at filling). These results demonstrate that the hybrid PROSAIL model holds significant promise for estimating LAI inversion in mulched winter wheat, providing a robust approach for phenotyping crop traits in ridge-furrow mulched systems.

Keywords:  mulched winter wheat       PROSAIL model              linear-spectral hybrid model              LAI              LASSO  
Online: 08 April 2026  
Fund: 

This study was funded by the National Natural Science Foundation of China for Young Scholars (5210906652579049), the China Postdoctoral Science Foundation (2023T160534 and 2022M712604), the Postdoctoral Science Foundation of Shaanxi Province, China (2023BSHTBZZ29), the National Key Research and Development Program (2023YFD190080504 and 2022YFD1900401), and the Key Research and Development Program of Shaanxi Province, China (2022NY-114).

About author:  Chunyu Wei, E-mail: 2019012003@nwafu.edu.cn; #Correspondence Yadan Du, E-mail: dyd123027@163.com

Cite this article: 

Chunyu Wei, Yadan Du, Yuxuan Liu, Xiaotao Hu, Zhikai Cheng, Yang Xu, Zhengtao Zhang, Xiaobo Gu, Kadambot H.M Siddique, Yunhui Niu. 2026. Optimizing leaf area index estimation in mulched winter wheat: A hybrid model approach coupling PROSAIL. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.04.006

Amirruddin A D, Muharam F M, Ismail M H, Tan N P, Ismail M F. 2022. Synthetic Minority Over-sampling TEchnique (SMOTE) and Logistic Model Tree (LMT)-Adaptive Boosting algorithms for classifying imbalanced datasets of nutrient and chlorophyll sufficiency levels of oil palm (Elaeis guineensis) using spectroradiometers and unmanned aerial vehicles. Computers and Electronics in Agriculture, 193, 106646.

Antonucci G, Impollonia G, Croci M, Potenza E, Marcone A, Amaducci S. 2023. Evaluating biostimulants via high-throughput field phenotyping: Biophysical traits retrieval through PROSAIL inversion. Smart Agricultural Technology, 3, 100067.

Baret F, Guyot G. 1991. Potentials and limits of vegetation indices for LAI and APAR assessment. Remote Sensing of Environment, 35, 161-173.

Berger K, Atzberger C, Danner M, D’Urso G, Mauser W, Vuolo F, Hank T. 2018. Evaluation of the PROSAIL model capabilities for future hyperspectral model environments: A review study. Remote Sensing, 10, 85.

Bioucas-Dias J M, Plaza A, Dobigeon N, Parente M, Du Q, Gader P, Chanussot J. 2012. Hyperspectral unmixing overview: Geometrical, statistical, and sparse regression-based approaches. IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 5, 354-379.

Chakhvashvili E, Siegmann B, Muller O, Verrelst J, Bendig J, Kraska T, Rascher U. 2022. Retrieval of crop variables from rroximal multispectral UAV image data using PROSAIL in maize Canopy. Remote Sensing, 14, 1247.

Chen P, Wang F. 2022. Effect of crop spectra purification on plant nitrogen concentration estimations performed using high-spatial-resolution images obtained with unmanned aerial vehicles. Field Crops Research, 288, 108708.

Cheng Z, Gu X, Du Y, Zhou Z, Li W, Zheng X, Cai W, Chang T. 2024a. Spectral purification improves monitoring accuracy of the comprehensive growth evaluation index for film-mulched winter wheat. Journal of Integrative Agriculture, 23, 1523-1540.

Cheng Z, Gu X, Wei C, Zhou Z, Zhao T, Wang Y, Li W, Du Y, Cai H. 2024b. Monitoring aboveground organs biomass of wheat and maize: A novel model combining ensemble learning and allometric theory. European Journal of Agronomy, 161, 127338.

Cheng Z, Gu X, Zhou Z, Zhang Y, Yin H, Li W, Chang T, Du Y. 2024c. Enhancing in-season yield forecast accuracy for film-mulched wheat: A hybrid approach coupling crop model and UAV remote-sensing data by ensemble learning technique. European Journal of Agronomy, 156, 127174.

Chlingaryan A, Sukkarieh S, Whelan B. 2018. Machine learning approaches for crop yield prediction and nitrogen status estimation in precision agriculture: A review. Computers and Electronics in Agriculture, 151, 61-69.

Darvishzadeh R, Skidmore A, Schlerf M, Atzberger C. 2008. Inversion of a radiative transfer model for estimating vegetation LAI and chlorophyll in a heterogeneous grassland. Remote Sensing of Environment, 112, 2592-2604.

Dash J, Curran P. 2004. The MERIS terrestrial chlorophyll index. International Journal of Remote Sensing, 25, 5403-5413.

Ding J, Wu J, Ding D, Yang Y, Gao C, Hu W. 2021. Effects of tillage and straw mulching on the crop productivity and hydrothermal resource utilization in a winter wheat-summer maize rotation system. Agricultral Water Management, 254, 106933.

Du R, Chen J, Xiang Y, Zhang Z, Yang N, Yang X, Tang Z, Wang H, Wang X, Shi H, Li W. 2023. Incremental learning for crop growth parameters estimation and nitrogen diagnosis from hyperspectral data. Computers and Electronics in Agriculture, 215, 108356.

Duan S B, Li Z L, Wu H, Tang B H, Ma L, Zhao E, Li C. 2014. Inversion of the PROSAIL model to estimate leaf area index of maize, potato, and sunflower fields from unmanned aerial vehicle hyperspectral data. International Journal of Applied Earth Observation and Geoinformation, 26, 12-20.

Féret J B, Gitelson A A, Noble S D, Jacquemoud S. 2017. PROSPECT-D: Towards modeling leaf optical properties through a complete lifecycle. Remote Sensing of Environment, 193, 204-215.

Féret J B, le Maire G, Jay S, Berveiller D, Bendoula R, Hmimina G, Cheraiet A, Oliveira J C, Ponzoni F J, Solanki T, de Boissieu F, Chave J, Nouvellon Y, Porcar-Castell A, Proisy C, Soudani K, Gastellu-Etchegorry J P, Lefèvre-Fonollosa M J. 2019. Estimating leaf mass per area and equivalent water thickness based on leaf optical properties: Potential and limitations of physical modeling and machine learning. Remote Sensing of Environment, 231, 110959.

Fang H. 2003. Retrieving leaf area index using a genetic algorithm with a canopy radiative transfer model. Remote Sensing of Environment, 85, 257-270.

Gao H, Yan C, Liu Q, Ding W, Chen B, Li Z. 2019. Effects of plastic mulching and plastic residue on agricultural production: A meta-analysis. Science of the Total Environment, 651, 484-492.

Gitelson A, Merzlyak M N. 1994. Quantitative estimation of chlorophyll-a using reflectance spectra: Experiments with autumn chestnut and maple leaves. Journal of Photochemistry and Photobiology (B: Biology), 22, 247-252.

Gitelson A A, Gritz Y, Merzlyak M N. 2003. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. Journal of Plant Physiology, 160, 271-282.

Gitelson A A, Kaufman Y J, Stark R, Rundquist D. 2002. Novel algorithms for remote estimation of vegetation fraction. Remote Sensing of Environment, 80, 76-87.

Guo A, Ye H, Huang W, Qian B, Wang J, Lan Y, Wang S. 2023. Inversion of maize leaf area index from UAV hyperspectral and multispectral imagery. Computers and Electronics in Agriculture, 212, 108020.

Hapke B. 2012. Theory of Reflectance and Emittance Spectroscopy. 2th ed. Cambridge University Press, UK.

Hasituya, Chen Z, Wang L, Wu W, Jiang Z, Li H. 2016. Monitoring plastic-mulched farmland by Landsat-8 OLI imagery using spectral and textural features. Remote Sensing8, 353.

Hauser L T, Féret J B, An Binh N, van der Windt N, Sil  F, Timmermans J, Soudzilovskaia N A, van Bodegom P M. 2021. Towards scalable estimation of plant functional diversity from Sentinel-2: In-situ validation in a heterogeneous (semi-)natural landscape. Remote Sensing of Environment, 262, 112505.

Houborg R, McCabe M F. 2018. A hybrid training approach for leaf area index estimation via Cubist and random forests machine-learning. ISPRS Journal of Photogrammetry and Remote Sensing, 135, 173-188.

Huete A, Didan K, Miura T, Rodriguez E P, Gao X, Ferreira L G. 2002. Overview of the radiometric and biophysical performance of the MODIS vegetation indices. Remote Sensing of Environment, 83, 195-213.

Huete A R. 1988. A soil-adjusted vegetation index (SAVI). Remote Sensing of Environment, 25, 295-309.

Jacquemoud S, Verhoef W, Baret F, Bacour C, Zarco-Tejada P J, Asner G P, François C, Ustin S L. 2009. PROSPECT+SAIL models: A review of use for vegetation characterization. Remote Sensing of Environment, 113, S56-S66.

Jay S, Maupas F, Bendoula R, Gorretta N. 2017. Retrieving LAI, chlorophyll and nitrogen contents in sugar beet crops from multi-angular optical remote sensing: Comparison of vegetation indices and PROSAIL inversion for field phenotyping. Field Crops Research, 210, 33-46.

Jiang H, Wei X, Chen Z, Zhu M, Yao Y, Zhang X, Jia K. 2023. Influence of different soil reflectance schemes on the retrieval of vegetation LAI and FVC from PROSAIL in agriculture region. Computers and Electronics in Agriculture, 212, 108165.

Jiang Z, Huete A, Didan K, Miura T. 2008. Development of a two-band enhanced vegetation index without a blue band. Remote Sensing of Environment, 112, 3833-3845.

Jiao Q J, Sun Q, Zhang B, Huang W J, Ye H C, Zhang Z M, Zhang X, Qian B X. 2022. A random forest algorithm for retrieving canopy chlorophyll content of wheat and soybean trained with PROSAIL simulations using adjusted average leaf angle. Remote Sensing14, 98.

Kumar S, Attri S D, Singh K K. 2019. Comparison of Lasso and stepwise regression technique for wheat yield prediction. Journal of Agrometeorology, 21, 188-192.

Li H, Dai J, Xiao J, Zou X, Chen T, Holmose M. 2022. Spectral variable selection based on least absolute shrinkage and selection operator with ridge-adding homotopy. Chemometrics and Intelligent Laboratory Systems, 221, 104487.

Liang L, Di L, Zhang L, Deng M, Qin Z, Zhao S, Lin H. 2015. Estimation of crop LAI using hyperspectral vegetation indices and a hybrid inversion method. Remote Sensing of Environment, 165, 123-134.

Liu T, Duan S B, Liu N, Wei B, Yang J, Chen J, Zhang L. 2024. Estimation of crop leaf area index based on Sentinel-2 images and PROSAIL-Transformer coupling model. Computers and Electronics in Agriculture, 227, 109663.

Liu Y, Ma X, An L, Sun H, Zhao F, Yan X, Ma Y, Li M. 2025. Exploring UAV narrow-band hyperspectral indices and crop functional traits derived from radiative transfer models to detect wheat powdery mildew. International Journal of Applied Earth Observation and Geoinformation, 141, 104627.

Luo L, Wang Z, Huang M, Hui X, Wang S, Zhao Y, He H, Zhang X, Diao C, Cao H, Ma Q, Liu J. 2018. Plastic film mulch increased winter wheat grain yield but reduced its protein content in dryland of northwest China. Field Crops Research, 218, 69-77.

Ma D, Chen L, Qu H, Wang Y, Misselbrook T, Jiang R. 2018. Impacts of plastic film mulching on crop yields, soil water, nitrate, and organic carbon in Northwestern China: A meta-analysis. Agricultural Water Management, 202, 166-173.

Machwitz M, Pieruschka R, Berger K, Schlerf M, Aasen H, Fahrner S, Jiménez-Berni J, Baret F, Rascher U. 2021. Bridging the gap between remote sensing and plant phenotyping-Challenges and opportunities for the next generation of sustainable agriculture. Frontiers in Plant Science, 12, 749374.

Mao W, Wang Y, Wang Y R. 2003. Real-time Detection of Between-row Weeds Using Machine Vision. 2003 American Society of Agriculture and Biological Engineers Annual Meeting. American Society of Agriculture and Biological Engineers, Michigan. pp 031004.

Maresma Á, Lloveras J, Martínez-Casasnovas J A. 2018. Use of multispectral airborne images to improve in-season nitrogen management, predict grain yield and estimate economic return of maize in irrigated high yielding environments. Remote Sensing, 10, 543.

Mulla D J. 2013. Twenty five years of remote sensing in precision agriculture: Key advances and remaining knowledge gaps. Biosystems Engineering, 114, 358-371.

Parker G G. 2020. Tamm review: Leaf area index (LAI) is both a determinant and a consequence of important processes in vegetation canopies. Forest Ecology and Management, 477, 118496.

Peng Y, Nguy-Robertson A, Arkebauer T, Gitelson A A. 2017. Assessment of canopy chlorophyll content retrieval in maize and soybean: Implications of hysteresis on the development of generic algorithms. Remote Sensing, 9, 116-125.

Peng Y, Zhu T E, Li Y C, Dai C, Fang S H, Gong Y, Wu X T, Zhu R S, Liu K. 2019. Remote prediction of yield based on LAI estimation in oilseed rape under different planting methods and nitrogen fertilizer applications. Agrcultural Forest Meteorology, 271, 116-125.

Prudnikova E, Savin I, Vindeker G, Grubina P, Shishkonakova E, Sharychev D. 2019. Influence of soil background on spectral reflectance of winter wheat crop canopy. Remote Sensing11, 1932.

Punalekar S M, Verhoef A, Quaife T L, Humphries D, Bermingham L, Reynolds C K. 2018. Application of Sentinel-2A data for pasture biomass monitoring using a physically based radiative transfer model. Remote Sensing of Environment, 218, 207-220.

Qu Y, Gao Z, Shang J, Liu J, Casa R. 2021. Simultaneous measurements of corn leaf area index and mean tilt angle from multi-directional sunlit and shaded fractions using downward-looking photography. Computers and Electronics in Agriculture, 180, 105881.

Reyniers M, Walvoort D, Baardemaaker. 2006. A linear model to predict with a multi-spectral radiometer the amount of nitrogen in winter wheat. International Journal of Remote Sensing, 27, 4159-4179.

Rondeaux G, Steven M, Baret F. 1996. Optimization of soil-adjusted vegetation indices. Remote Sensing of Environment, 55, 95-107.

Roosjen P P J, Brede B, Suomalainen J M, Bartholomeus H M, Kooistra L, Clevers J G P W. 2018. Improved estimation of leaf area index and leaf chlorophyll content of a potato crop using multi-angle spectral data – potential of unmanned aerial vehicle imagery. International Journal of Applied Earth Observation and Geoinformation, 66, 14-26.

Roujean J L, Breon F M. 1995. Estimating PAR absorbed by vegetation from bidirectional reflectance measurements. Remote Sensing of Environment, 51, 375-384.

Rouse J W, Haas R H, Schell J A, Deering D W. 1973. Monitoring vegetation systems in the great plains with ERTS, in, 1973.

Sehgal V K, Chakraborty D, Sahoo R N. 2016. Inversion of radiative transfer model for retrieval of wheat biophysical parameters from broadband reflectance measurements. Information Processing in Agriculture, 3, 107-118.

Si Y, Schlerf M, Zurita-Milla R, Skidmore A, Wang T. 2012. Mapping spatio-temporal variation of grassland quantity and quality using MERIS data and the PROSAIL model. Remote Sensing of Environment, 121, 415-425.

Sinha S K, Padalia H, Dasgupta A, Verrelst J, Rivera J P. 2020. Estimation of leaf area index using PROSAIL based LUT inversion, MLRA-GPR and empirical models: Case study of tropical deciduous forest plantation, North India. International Journal of Applied Earth Observation and Geoinformation, 86, 102027.

Song G, Wang Q. 2023. Coupling effective variable selection with machine learning techniques for better estimating leaf photosynthetic capacity in a tree species (Fagus crenata Blume) from hyperspectral reflectance. Agrcultural Forest Meteorology, 338, 109528.

Sun Q, Jiao Q, Qian X, Liu L, Liu X, Dai H. 2021. Improving the retrieval of crop canopy chlorophyll content using vegetation index combinations. Remote Sensing, 13, 470.

Sun Y, Qin Q, Ren H, Zhang T, Chen S. 2020. Red-edge band vegetation indices for leaf area index estimation from Sentinel-2/MSI Imagery. IEEE Transactions on Geoscience and Remote Sensing, 58, 826-840.

Tibshirani R. 2011. Regression shrinkage and selection via the Lasso: A retrospective. Journal of the Royal Statistical Society Series B: Statistical Methodology, 73, 273-282.

Verhoef W, Jia L, Xiao Q, Su Z. 2007. Unified optical-thermal four-stream radiative transfer theory for homogeneous vegetation canopies. IEEE Transactions on Geoscience and Remote Sensing, 45, 1808-1822.

Verrelst J, Camps-Valls G, Muñoz-Marí J, Rivera J P, Veroustraete F, Clevers J G P W, Moreno J. 2015. Optical remote sensing and the retrieval of terrestrial vegetation bio-geophysical properties–A review. ISPRS Journal of Photogrammetry and Remote Sensing, 108, 273-290.

Verrelst J, Muñoz J, Alonso L, Delegido J, Rivera J P, Camps-Valls G, Moreno J. 2012. Machine learning regression algorithms for biophysical parameter retrieval: Opportunities for Sentinel-2 and-3. Remote Sensing of Environment, 118, 127-139.

Viña A, Gitelson A A, Nguy-Robertson A L, Peng Y. 2011. Comparison of different vegetation indices for the remote assessment of green leaf area index of crops. Remote Sensing of Environment, 115, 3468-3478.

Wang B, Jia K, Liang S, Xie X, Wei X, Zhao X, Yao Y, Zhang X. 2018. Assessment of Sentinel-2 MSI spectral band reflectances for estimating fractional vegetation cover. Remote Sensing, 10, 1927.

Wang X, Li X, Yang Z, Gao Z, Wang L, Zhang B. 2022. Retrieving method for leaf area index of winter wheat by combining PROSAIL model with VMG model. Transactions of the Chinese Society for Agriculture Machinery, 53, 209-216.

Wu L, Liu X, Wang P, Zhou B, Liu M, Li X. 2013. The assimilation of spectral sensing and the WOFOST model for the dynamic simulation of cadmium accumulation in rice tissues. International Journal of Applied Earth Observation and Geoinformation, 25, 66-75.

Wu Z, Zhao C, Qin Q. 2022. Evaluating the impact of spatial heterogeneity on the prosail model and lai inversion. International Geoscience and Remote Sensing Symposium 2022-2022 Institute of Electrical and Electronics Engineers International. Institute of Electrical and Electronics Engineers, Malaysia. pp. 6260-6263.

Xue J, Su B. 2017. Significant remote sensing vegetation indices: A review of developments and applications. Journal of Sensors, 2017, 1-17.

Yadav V P, Prasad R, Bala R. 2019. Leaf area index estimation of wheat crop using modified water cloud model from the time-series SAR and optical satellite data. Geocarto International, 36, 791-802.

Yang N, Zhang Z, Yang X, Zhang J, Zhang B, Xie P, Wang Y, Chen J, Shi L. 2025. UAV-based stomatal conductance estimation under water stress using the PROSAIL model coupled with meteorological factors. International Journal of Applied Earth Observation and Geoinformation, 137, 104425.

Yue J, Yang G, Li C, Liu Y, Wang J, Guo W, Ma X, Niu Q, Qiao H, Feng H. 2024. Analyzing winter-wheat biochemical traits using hyperspectral remote sensing and deep learning. Computers and Electronics in Agriculture, 222, 109026.

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