园艺-分子生物合辑Horticulture — Genetics · Breeding
|Genome-wide identification and expression analysis of GDSL esterase/lipase genes in tomato
|SUN Yao-guang, HE Yu-qing, WANG He-xuan, JIANG Jing-bin, YANG Huan-huan, XU Xiang-yang
|School of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, P.R.China
GDSL酯酶/脂肪酶家族包含许多功能基因，它们在植物的生长发育、形态建成、种子油脂合成和防御反应中发挥重要的生物学功能。GDSL酯酶/脂肪酶基因可响应生物和非生物胁迫。虽然GDSL酯酶/脂肪酶家族基因在其他植物中已被鉴定和研究，但它们在番茄中的分类和功能尚不清楚。本研究首次在番茄中鉴定了80个GDSL酯酶/脂肪酶家族基因成员，命名为SlGELP1-80。我们对这些基因在染色体上的位置进行了定位，并对它们的理化性质、基因结构、系统发育关系、共线关系和顺式作用元件进行了分析。SlGELP基因在番茄中的时空表达特征具有多样性。此外，结合RNA-seq分析表明，番茄接种Stemphylium lycopersici前后SlGELP基因的表达模式不同。用qRT-PCR方法验证了番茄接种S. lycopersici病原菌以及分别喷施SA和JA处理后5个显著差异SlGELP基因的表达。本研究首次利用生物信息学方法鉴定和分析了番茄GDSL酯酶/脂肪酶家族基因，为提高植物抗病性研究提供了新的思路。
Abstract The GDSL esterase/lipase family contains many functional genes that perform important biological functions in growth and development, morphogenesis, seed oil synthesis, and defense responses in plants. The expression of GDSL esterase/lipase genes can respond to biotic and abiotic stresses. Although GDSL esterase/lipase family genes have been identified and studied in other plants, they have not been identified and their functions remain unclear in tomato. This study is the first to identify 80 GDSL esterase/lipase family genes in tomato, which were named SlGELP1–80. These genes were mapped to their positions on the chromosomes and their physical and chemical properties, gene structure, phylogenetic relationships, collinear relationships, and cis-acting elements were analyzed. The spatiotemporal expression characteristics of the SlGELP genes in tomato were diverse. In addition, RNA-seq analysis indicated that the expression patterns of the SlGELP genes in tomato differed before and after inoculation with Stemphylium lycopersici. qRT-PCR was used to analyze the expression of five SlGELP genes after treatments with S. lycopersici, salicylic acid and jasmonic acid. Finally, this study was the first to identify and analyze GDSL esterase/lipase family genes in tomato via bioinformatics approaches, and these findings provide new insights for improving the study of plant disease resistance.
Received: 02 July 2020
Accepted: 16 October 2020
|Fund: This work was supported by the “BaiQianWan” Project of Heilongjiang Province, China (2019ZX16B02), the National Natural Science Foundation of China (32002059), the Heilongjiang Natural Science Foundation of China (LH2020C10), and the Fellowship of China Postdoctoral Science Foundation (2020M681068).
|About author: SUN Yao-guang, E-mail: firstname.lastname@example.org; Correspondence YANG Huan-huan, E-mail: email@example.com; XU Xiang-yang, Tel/Fax: +86-451-55190748, E-mail: firstname.lastname@example.org
Cite this article:
SUN Yao-guang, HE Yu-qing, WANG He-xuan, JIANG Jing-bin, YANG Huan-huan, XU Xiang-yang.
Genome-wide identification and expression analysis of GDSL esterase/lipase genes in tomato. Journal of Integrative Agriculture, 21(2): 389-406.
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