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Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2369-2379    DOI: 10.1016/S2095-3119(15)61272-2
Soil & Fertilization﹒Irrigation﹒Plant Nutrition﹒ Agro-Ecology & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Screening of a microbial consortium with efficient corn stover degradation ability at low temperature
Qinggeer1, GAO Ju-lin1, YU Xiao-fang1, ZHANG Bao-lin2, WANG Zhi-gang1, Borjigin Naoganchaolu1, HU Shu-ping1, SUN Ji-ying1, XIE Min1, WANG Zhen1
1 Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R.China
2 Chemistry and Environmental Science College, Inner Mongolia Normal University, Hohhot 010019, P.R.China
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Abstract      To speed up the degradation of corn stover directly returned to soil at low temperature, the corn stover-degrading microbial consortium GF-20, acclimated to biological decomposition in the frigid region, was successfully constructed under a long-term limiting substrate. To evaluate its potential in accelerating the decomposition of un-pretreated corn stover, the decomposing property, fermentation dynamic and the microbial diversity were analyzed. GF-20 degraded corn stover by 32% after 15-day fermentation at 10°C. Peak activities of filter paperlyase (FPA), β-glucosidases (CB), endoglucanases (Cx), and cellobiohydrolases (C1) were 1.15, 1.67, 1.73, and 1.42 U mL–1, appearing at the 6th, 3rd, 11th, and 9th d, respectively. The pH averaged at 6.73–8.42, and the optical density (OD) value peaked at 1.87 at the 120 h of the degradation process. Cellulase, hemicellulase and lignin in corn stover were persistently degraded by 44.85, 43.85 and 25.29% at the end of incubation. Result of denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that GF-20 had a stable component structure under switching the temperature and pH. The composition of the GF-20 was also analyzed by constructing bacterial 16S rDNA clone library and fungal 18SrDNA-PCR-DGGE. Twenty-two bacterial clones and four fungal bands were detected and identified dominant bacteria represented by Cellvibrio mixtus subsp., Azospira oryzae, Arcobacter defluyii, and Clostridium populeti and the fungi were mainly identified as related to Trichosporon sp.
Keywords:  corn stover        degradation        microbial consortium        low temperature  
Received: 24 July 2015   Accepted:
Fund: 

  This study was supported by the National Natural Science Foundation of China (31260300), the National Maize Industrial Technology Systems, China (CARS-02-63), the Science & Technology Project for Food Production, China (2011BAD16B13, 2012BAD04B04, 2013BAD07B04), the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120).

Corresponding Authors:  GAO Ju-lin, Tel: +86-471-4301317, E-mail: nmgaojulin@163.com   

Cite this article: 

Qinggeer , GAO Ju-lin, YU Xiao-fang, ZHANG Bao-lin, WANG Zhi-gang, Borjigin Naoganchaolu, HU Shu-ping, SUN Ji-ying, XIE Min, WANG Zhen. 2016. Screening of a microbial consortium with efficient corn stover degradation ability at low temperature. Journal of Integrative Agriculture, 15(10): 2369-2379.

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