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Journal of Integrative Agriculture  2020, Vol. 19 Issue (6): 1554-1564    DOI: 10.1016/S2095-3119(19)62779-6
Special Issue: 玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources
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The effect of amylose on kernel phenotypic characteristics, starch-related gene expression and amylose inheritance in naturally mutated high-amylose maize
ZHANG Xu-dong1, 2*, GAO Xue-chun1*, LI Zhi-wei1, XU Lu-chun1, LI Yi-bo1, ZHANG Ren-he1, XUE Ji-quan1, GUO Dong-wei1
1 Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs/College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
2 Institute of Crop Science, Quality of Plant Products, University of Hohenheim, Stuttgart 70599, Germany
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Abstract  
High-amylose maize starch has great potential for widespread industrial use due to its ability to form strong gels and film and in the food processing field, thus serving as a resistant starch source.  However, there is still a substantial shortage of high-amylose maize due to the limitation of natural germplasm resources, although the well-known amylose extender (ae) gene mutants have been found to produce high-amylose maize lines since 1948.  In this context, high-amylose maize lines (13 inbreds and 18 hybrids) originating from a natural amylose mutant in our testing field were utilized to study the correlation between amylose content (AC) and phenotypic traits (kernel morphology and endosperm glossiness), grain filling characteristics, gene expression, and amylose inheritance.  Our results showed that AC was negatively correlated with total starch content but was not correlated with grain phenotypes, such as kernel fullness, kernel morphology and endosperm glossiness.  Maize lines with higher amylose had a greater grain filling rate than that of the control (B73) during the first 20 days after pollination (DAP).  Both starch debranching enzyme (DBE) groups and starch branching enzyme IIb (SBEIIb) groups showed a greater abundance in the control (B73) than in the high-amylose maize lines.  Male parents directly predicted AC of F1, which was moderately positively correlated with the F2 generation.
 
Keywords:  starch biosynthesis        amylose inheritance        high-amylose maize        grain filling rate  
Received: 19 February 2019   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0300304), the Sci-Tech Project of Yangling City, China (2014NY-01), the Tang Foundation, China (A212021205), and the Shaanxi Science & Technology Co-ordination & Innovation Project, China (2015KTZDNY01-01-01).
Corresponding Authors:  Correspondence GUO Dong-wei, Tel: +86-29-87082934, Fax: +86-29-87082854, E-mail: gdwei@nwsuaf.edu.cn    
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHANG Xu-dong, GAO Xue-chun, LI Zhi-wei, XU Lu-chun, LI Yi-bo, ZHANG Ren-he, XUE Ji-quan, GUO Dong-wei. 2020. The effect of amylose on kernel phenotypic characteristics, starch-related gene expression and amylose inheritance in naturally mutated high-amylose maize. Journal of Integrative Agriculture, 19(6): 1554-1564.

Armstrong P R, Tallada J G, Hurburgh C, Hildebrand D F, Specht J E. 2011. Development of single-seed near-infrared spectroscopic predictions of corn and soybean constituents using bulk reference values and mean spectra. Transactions of the American Society of Agricultural and Biological Engineers, 54, 1529–1535.
Bear R, Vineyard M, MacMasters M, Deatherage W. 1958. Development of “amylomaize” - corn hybrids with high amylose starch: ii. Results of breeding efforts 1. Agronomy Journal, 50, 598–602.
Bhattacharyya M K, Smith A M, Ellis T N, Hedley C, Martin C. 1990. The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme. Cell, 60, 115–122.
Boyer C D, Preiss J. 1978. Multiple forms of starch branching enzyme of maize: Evidence for independent genetic control. Biochemical and Biophysical Research Communications. 80, 169–175.
Boyer C D, Preiss J. 1981. Evidence for independent genetic control of the multiple forms of maize endosperm branching enzymes and starch synthases. Plant Physiology, 67, 1141–1145.
Cameron J W. 1947. Chemico-genetic bases for the reserve carbohydrates in maize endosperm. Genetics, 32, 459.
Cao H, Imparl-Radosevich J, Guan H, Keeling P L, James M G, Myers A M. 1999. Identification of the soluble starch synthase activities of maize endosperm. Plant Physiology, 120, 205–216.
Cárcova J, Otegui M E. 2001. Ear temperature and pollination timing effects on maize kernel set. Crop Science, 41, 1809–1815.
Case S, Capitani T, Whaley J, Shi Y, Trzasko P, Jeffcoat R, Goldfarb H. 1998. Physical properties and gelation behavior of a low-amylopectin maize starch and other high-amylose maize starches. Journal of Cereal Science, 27, 301–314.
Dinges J R, James M G, Myers A M. 2003. Genetic analysis indicates maize pullulanase-and isoamylase-type starch debranching enzymes have partially overlapping functions in starch metabolism. Journal of Applied Glycoscience, 50, 191–195.
Doebley J F, Gaut B S, Smith B D. 2006. The molecular genetics of crop domestication. Cell, 127, 1309–1321.
Duguid S, Brule-Babel A. 1994. Rate and duration of grain filling in five spring wheat (Triticum aestivum L.) genotypes. Canadian Journal of Plant Science, 74, 681–686.
Dumez S, Wattebled F, Dauvillee D, Delvalle D, Planchot V, Ball S G, D’Hulst C. 2006. Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation. The Plant Cell, 18, 2694–2709.
Dunn G, Kramer H, Whistler R L. 1953. Gene dosage effects on corn endosperm carbohydrates 1. Agronomy Journal, 45, 101–104.
Gao M, Fisher D K, Kim K N, Shannon J C, Guiltinan M J. 1997. Independent genetic control of maize starch-branching enzymes IIa and IIb (isolation and characterization of a Sbe2a cDNA). Plant Physiology, 114, 69–78.
Guan H, Dong Y, Liu C, He C, Liu C, Liu Q, Dong R, Li Y, Liu T, Wang L. 2017. A splice site mutation in shrunken1-m causes the shrunken 1 mutant phenotype in maize. Plant Growth Regulation, 83, 429–439.
Jiang Q, Du Y, Tian X, Wang Q, Xiong R, Xu G, Yan C, Ding Y. 2016. Effect of panicle nitrogen on grain filling characteristics of high-yielding rice cultivars. European Journal of Agronomy, 74, 185–192.
Knight M E, Harn C, Lilley C E, Guan H, Singletary G W, Mu-Forster C, Wasserman B P, Keeling P L. 1998. Molecular cloning of starch synthase I from maize (W64) endosperm and expression in Escherichia coli. The Plant Journal, 14, 613–622.
Kramer H, Bear R, Zuber M. 1958. Designation of high amylose gene loci in maize. Agronomy Journal, 50, 229–229.
Kramer H H, Whistler R L. 1949. Quantitative effects of certain genes on the amylose content of corn endosperm starch. Agronomy Journal, 41, 409–411.
Lin L, Guo D, Zhao L, Zhang X, Wang J, Zhang F, Wei C. 2016. Comparative structure of starches from high-amylose maize inbred lines and their hybrids. Food Hydrocolloids, 52, 19–28.
Liu Z, Ji H, Cui Z, Wu X, Duan L, Feng X, Tang J. 2011. QTL detected for grain-filling rate in maize using a RIL population. Molecular Breeding, 27, 25–36.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402–408.
Ma Y, Chen Y, Zhu J, Meng L, Guo Y, Li B, Hoogenboom G. 2018. Coupling individual kernel-filling processes with source-sink interactions into GREENLAB-maize. Annals of Botany, 121, 961–973.
McClintock B. 1948. Mutable loci in maize. Carnegie Institute of Washington, Year Book, 47, 155–169.
McClintok B. 1954. Mutations in maize and chromosomal aberrations in neurospora. Carnegie Institute of Washington, Year Book, 53, 254–260.
Nannas N J, Dawe R K. 2015. Genetic and genomic toolbox of Zea mays. Genetics, 199, 655–669.
Nelson O, Pan D. 1995. Starch synthesis in maize endosperms. Annual Review of Plant Biology, 46, 475–496.
Nelson O E, Rines H W. 1962. The enzymatic deficiency in the waxy mutant of maize. Biochemical and Biophysical Research Communications, 9, 297–300.
Pan D, Nelson O E. 1984. A debranching enzyme deficiency in endosperms of the sugary-1 mutants of maize. Plant Physiology, 74, 324–328.
Patron N J, Smith A M, Fahy B F, Hylton C M, Naldrett M J, Rossnagel B G, Denyer K. 2002. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in the 5´-non-coding region. Plant Physiology, 130, 190–198.
Pfister B, Zeeman S C. 2016. Formation of starch in plant cells. Cellular and Molecular Life Sciences, 73, 2781–2807.
Polaske N W, Wood A L, Campbell M R, Nagan M C, Pollak L M. 2005. Amylose determination of native high-amylose corn starches by differential scanning calorimetry. Starch-Stärke, 57, 118–123.
Pratt R, Paulis J, Miller K, Nelsen T, Bietz J. 1995. Association of zein classes with maize kernel hardness. Cereal Chemistry, 72, 162–166.
Singh A K, Sharma V, Pal A K, Acharya V, Ahuja P S. 2013. Genome-wide organization and expression profiling of the NAC transcription factor family in potato (Solanum tuberosum L.). DNA Research, 20, 403–423.
Sui Z, Huber K C, BeMiller J N. 2013. Effects of the order of addition of reagents and catalyst on modification of maize starches. Carbohydrate Polymers, 96, 118–130.
Sun C, Sathish P, Ahlandsberg S, Jansson C. 1998. The two genes encoding starch-branching enzymes IIa and IIb are differentially expressed in barley. Plant Physiology, 118, 37–49.
Tester R F, Karkalas J, Qi X. 2004. Starch - composition, fine structure and architecture. Journal of Cereal Science, 39, 151–165.
Tsai C Y. 1974. The function of the waxy locus in starch synthesis in maize endosperm. Biochemical Genetics, 11, 83–96.
Vineyard M, Bear R, MacMasters M, Deatherage W. 1958. Development of “amylomaize” - corn hybrids with high amylose starch: I. genetic considerations 1. Agronomy Journal, 50, 595–598.
Wu Y, Campbell M, Yen Y, Wicks Z, Ibrahim A M. 2009. Genetic analysis of high amylose content in maize (Zea mays L.) using a triploid endosperm model. Euphytica, 166, 155–164.
Yamanouchi H, Nakamura Y. 1992. Organ specificity of isoforms of starch branching enzyme (Q-enzyme) in rice. Plant and Cell Physiology, 33, 985–991.
Yang J, Zhang H, Song X, Wang X. 2011. New method for qualifying and evaluating kernel fullness in maize by image process. Journal of Maize Sciences, 19, 148–152. (in Chinese)
Yang Y P, Juang Y S, Hsu B D. 2002. A quick method for assessing chloroplastic starch granules by flow cytometry. Journal of Plant Physiology, 159, 103–106.
Zhang X, Chen Y, Zhang R, Zhong Y, Luo Y, Xu S, Liu J, Xue J, Guo D. 2016. Effects of extrusion treatment on physicochemical properties and in vitro digestion of pregelatinized high amylose maize flour. Journal of Cereal Science, 68, 108–115.
Zhao Y, Li N, Li B, Li Z, Xie G, Zhang J. 2015. Reduced expression of starch branching enzyme IIa and IIb in maize endosperm by RNAi constructs greatly increases the amylose content in kernel with nearly normal morphology. Planta, 241, 449–461.
Zhu T, Jackson D S, Wehling R L, Geera B. 2008. Comparison of amylose determination methods and the development of a dual wavelength iodine binding technique. Cereal Chemistry, 85, 51–58.
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