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Journal of Integrative Agriculture  2020, Vol. 19 Issue (6): 1554-1564    DOI: 10.1016/S2095-3119(19)62779-6
Special Issue: 玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources
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The effect of amylose on kernel phenotypic characteristics, starch-related gene expression and amylose inheritance in naturally mutated high-amylose maize
ZHANG Xu-dong1, 2*, GAO Xue-chun1*, LI Zhi-wei1, XU Lu-chun1, LI Yi-bo1, ZHANG Ren-he1, XUE Ji-quan1, GUO Dong-wei1
1 Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs/College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
2 Institute of Crop Science, Quality of Plant Products, University of Hohenheim, Stuttgart 70599, Germany
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High-amylose maize starch has great potential for widespread industrial use due to its ability to form strong gels and film and in the food processing field, thus serving as a resistant starch source.  However, there is still a substantial shortage of high-amylose maize due to the limitation of natural germplasm resources, although the well-known amylose extender (ae) gene mutants have been found to produce high-amylose maize lines since 1948.  In this context, high-amylose maize lines (13 inbreds and 18 hybrids) originating from a natural amylose mutant in our testing field were utilized to study the correlation between amylose content (AC) and phenotypic traits (kernel morphology and endosperm glossiness), grain filling characteristics, gene expression, and amylose inheritance.  Our results showed that AC was negatively correlated with total starch content but was not correlated with grain phenotypes, such as kernel fullness, kernel morphology and endosperm glossiness.  Maize lines with higher amylose had a greater grain filling rate than that of the control (B73) during the first 20 days after pollination (DAP).  Both starch debranching enzyme (DBE) groups and starch branching enzyme IIb (SBEIIb) groups showed a greater abundance in the control (B73) than in the high-amylose maize lines.  Male parents directly predicted AC of F1, which was moderately positively correlated with the F2 generation.
Keywords:  starch biosynthesis        amylose inheritance        high-amylose maize        grain filling rate  
Received: 19 February 2019   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0300304), the Sci-Tech Project of Yangling City, China (2014NY-01), the Tang Foundation, China (A212021205), and the Shaanxi Science & Technology Co-ordination & Innovation Project, China (2015KTZDNY01-01-01).
Corresponding Authors:  Correspondence GUO Dong-wei, Tel: +86-29-87082934, Fax: +86-29-87082854, E-mail:    
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHANG Xu-dong, GAO Xue-chun, LI Zhi-wei, XU Lu-chun, LI Yi-bo, ZHANG Ren-he, XUE Ji-quan, GUO Dong-wei. 2020. The effect of amylose on kernel phenotypic characteristics, starch-related gene expression and amylose inheritance in naturally mutated high-amylose maize. Journal of Integrative Agriculture, 19(6): 1554-1564.

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