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Journal of Integrative Agriculture  2018, Vol. 17 Issue (09): 2126-2136    DOI: 10.1016/S2095-3119(18)62027-1
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Identification and characterization of Pichia membranifaciens Hmp-1 isolated from spoilage blackberry wine
WANG Ying1, ZHAO Yan-cun2, FAN Lin-lin1, XIA Xiu-dong1, LI Ya-hui1, ZHOU Jian-zhong
1 Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
2 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
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The pellicle-forming yeast could cause the quality deterioration of wine.  In this study, a pellicle-forming strain Hmp-1 was isolated from the spoilage blackberry wine, and identified as Pichia membranifaciens based on the morphology and partial nucleotide sequence of 26S rDNA.  The effects of fermentation conditions (ethanol, sulfur dioxide, sugar, and temperature) on the growth of P. membranifaciens strain Hmp-1 and Saccharomyces cerevisiae strain FM-S-115 (a strain used for the blackberry wine fermentation) were investigated, respectively.  The results indicated that Hmp-1 had lower resistance to these factors compared to FM-S-115, and the growth of Hmp-1 was completely inhibited by 10% (v/v) or 50 mg L–1 SO2 during the fermentation of blackberry wine.  These results suggested that Hmp-1 could effectively be controlled by increasing ethanol or SO2 concentration during the fermentation and storage of blackberry wine.  Furthermore, the analysis based on gas chromatography-mass spectrometry (GC-MS) showed that Hmp-1 remarkably decreased kinds of volatile compounds in blackberry wine, especially aldehydes and esters.  In addition, some poisonous compounds were detected in the blackberry wine fermented by FM-S-115 and Hmp-1.  These results suggested that Hmp-1 was a major cause leading to the quality deterioration of blackberry wine.
Keywords:  Picihia membranifaciens Hmp-1        blackberry wine        pellicle-forming spoilage        population dynamics        volatile compounds  
Received: 21 December 2017   Accepted: 14 August 2018
Fund: This work was supported by the Natural Science Foundation of Jiangsu Province, China (BK20170603).
Corresponding Authors:  Correspondence ZHOU Jian-zhong, Tel: +86-25-84392177, Fax: +86-25-84391677, E-mail:   
About author:  WANG Ying, E-mail:;

Cite this article: 

WANG Ying, ZHAO Yan-cun, FAN Lin-lin, XIA Xiu-dong, LI Ya-hui, ZHOU Jian-zhong. 2018. Identification and characterization of Pichia membranifaciens Hmp-1 isolated from spoilage blackberry wine. Journal of Integrative Agriculture, 17(09): 2126-2136.

Van der Aa Kühle A, Jespersen L. 2003. The taxonomic position of Saccharomyces boulardii as evaluated by sequence analysis of the D1/D2 domain of 26S rDNA, the ITS1-5.8SrDNA-ITS2 region and the mitochondrial cytochrome-c oxidase II gene. Systematic and Applied Microbiology, 26, 564–571.
Álvarez-Pérez J M, Campo E, San-Juan F J, Coque J, Ferreira V, Hernández-Orte P. 2012. Sensory and chemical characterisation of the aroma of Prieto Picudo rosé wines: The differential role of autochthonous yeast strains on aroma pro?les. Food Chemistry, 133, 284–292.
Asimakopoulos A G, Wang L, Thomaidis N S, Kannan K. 2013. Benzotriazoles and benzothiazoles in human urine from several countries: A perspective on occurrence, biotransformation, and human exposure. Environment International, 59, 274–281.
Bartowsky E J, Henschke P A. 2008. Acetic acid bacteria spoilage of bottled red wine - A review. International Journal of Food Microbiology, 125, 60–70.
Combina M, Elía A, Mercado L, Catania C, Ganga A, Martinez C. 2005. Dynamics of indigenous yeast populations during spontaneous fermentation of wines from Mendoza, Argentina. International Journal of Food Microbiology, 99, 237–243.
Contreras A, Hidalgo C, Henschke P A, Chambers P J, Curtin C, Varela C. 2014. Evaluation of non-Saccharomyces yeasts for the reduction of alcohol content in wine. Applied and Environmental Microbiology, 80, 1670–1678.
Cordente A G, Curtin C D, Varela C, Pretorius I S. 2012. Flavour-active wine yeasts. Applied Microbiology and Biotechnology, 96, 601–618.
Díaz C, Molina A M, Nähring J, Fischer R. 2013. Characterization and dynamic behavior of wild yeast during spontaneous wine fermentation in steel tanks and amphorae. BioMed Research International, 2013, 540465.
Elisia I, Hu C, Popovich D G, Kitts D D. 2007. Antioxidant assessment of an anthocyanin-enriched blackberry extract. Food Chemistry, 101, 1052–1058.
Enrique M, Marcos J F, Yuste M, Martínez M, Vallés S, Manzanares P. 2008. Inhibition of the wine spoilage yeast Dekkera bruxellensis by bovine lactoferrin-derived peptides. International Journal of Food Microbiology, 127, 229–234.
Farkas A, Butiuc-Keul A, Ciatarâ? D, Neam?u C, Cr?ciuna? C, Podar D, Dr?gan-Bularda M. 2013. Microbiological contamination and resistance genes in bio?lms occurring during the drinking water treatment process. Science of the Total Environment, 443, 932–938.
Fleet G H. 2003. Yeast interactions and wine flavour. International Journal of Food Microbiology, 86, 11–22.
Fugelsang K C, Edwards C G. 2007. Managing microbial growth.  In: Wine Microbiology. Practical Applications and Procedures. 2nd ed. Springer Science, New York. pp. 65–80.
Ibeas J I, Jimenez J. 1997. Mitochondrial DNA loss caused by ethanol in Saccharomyces ?or yeasts. Applied and Environmental Microbiology, 63, 7–12.
Jackowetz J N, Mira de Orduña R. 2013. Survey of SO2 binding carbonyls in 237 red and white table wines. Food Control, 32, 687–692.
Janna H, Scrimshaw M D, Williams R J, Churchley J, Sumpter J P. 2011. From dishwasher to tap? Xenobiotic substances benzotriazole and tolyltriazole in the environment. Environmenysl Science & Technology, 45, 3858–3864.
Jiang B, Xi Z M, Luo M J, Zhang Z W. 2013. Comparison on aroma compounds in Cabernet Sauvignon and Merlot wines from four wine grape-growing regions in China. Food Research International, 51, 482–489.
Johnson M H, Gonzalez de Mejia E. 2012. Comparison of chemical composition and antioxidant capacity of commercially available blueberry and blackberry wines in Illinois. Journal of Food Science, 71, 141–148.
Loureiro V, Malfeito-Ferreira M. 2003. Spoilage yeast in the wine industry. International Journal of Food Microbiology, 86, 23–50.
Maro E D, Ercolini D, Coppola S. 2007. Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape. International Journal of Food Microbiology, 117, 201–210.
Miguel M G C P, Cardoso P G, Magalhães-Guedes K T, Schwa, R F. 2013. Identification and assessment of kefir yeast potential for sugar/ethanol-resistance. Brazilian Journal of Microbiology, 44, 113–118.
Nakagawa Y, Toda Y, Yamamura H, Hayakawa M, Iimura Y. 2011. FLO11 is essential for pellicle formation by wild pellicle-forming yeasts isolated from contaminated wines. Journal of Bioscience Bioengineering, 111, 7–9.
Oelofse A, Pretorius I S, Du Toit M. 2008. Signi?cance of Brettanomyces and Dekkera during winemaking: A synoptic review. South African Journal Enology Viticulture, 29, 128–144.
Ortiz J, Marín Arroyo M R, Noriega Domínguez M J, Navarro M, Arozarena I. 2013. Color, phenolics, and antioxidant activity of blackberry (Rubus glaucus Benth.), blueberry (Vaccinium ?oribundum Kunth.), and apple wines from Ecuador. Journal of Food Science, 78, 985–993.
Pal A, He Y L, Jekel M, Reinhard M, Gin K Y H. 2014. Emerging contaminants of public health signi?cance as water quality indicator compounds in the urban water cycle. Environment International, 71, 46–62.
Parlapani F F, Haroutounian S A, Nychas G E, Boziaris I S. 2015. Microbiological spoilage and volatiles production of gutted European sea bass stored under air and commercial modi?ed atmosphere package at 2°C. Food Microbiology, 50, 44–53.
Parlapani F F, Mallouchos A, Haroutounian S A, Boziaris I S. 2014. Microbiological spoilage and investigation of volatile pro?le during storage of sea bream ?llets under various conditions. International Journal of Food Microbiology, 189,153–163.
Petri A, Pfannebecker J, Fröhlich J, König H. 2013. Fast identi?cation of wine related lactic acid bacteria by multiplex PCR. Food Microbiology, 33, 48–54.
Rankine B C. 1968. The importance of yeast in determining the composition and quality of wines. Vitis, 7, 22–49.
Saez J S, Lopes C A, Kirs V E, Sangorrín M. 2011. Production of volatile phenols by Pichia manshurica and Pichia membranifaciens isolated from spoiled wines and cellar environment in Patagonia. Food Microbiology, 28, 503–509.
Selli S, Cabaroglu T, Canbas A, Erten H, Nurgel C, Lepoutre J P, Gunata Z. 2004. Volatile composition of red wine from cv. Kalecik Karasι grown in central Anatolia. Food Chemistry, 85, 207–213.
De Souza V R, Pereira P A, Da Silva T L, De Oliveira Lima L C, Pioe, R, Queiroz F. 2014. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chemistry, 156, 362–368.
Sponholz W F. 1992. Wine spoilage by microorganisms. In: Flee G H, ed., Wine Microbiology and Biotechnology. Harwood Academic Publishers, Amsterdam. pp. 395–419.
Sturm M E, Arroyo-López F N, Garrido-Fernández A, Querol A, Mercado L A, Ramirez M L, Combina M. 2014. Probabilistic model for the spoilage wine yeast Dekkera bruxellensis as a function of pH, ethanol and free SO2 using time as a dummy variable. International Journal of Food Microbiology, 170, 83–90.
Suárez R, Suárez-Lepe J A, Morata A, Calderón F. 2007. The production of ethylphenols in wine by yeast of genera Brettanomyces and Dekkera: A review. Food Chemistry, 102, 10–21.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The Clustal_X windows interface: Fexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876–4882.
Tristezza M, di Feo L, Tufariello M, Grieco F, Capozzi V, Spano G, Mita G, Grieco F. 2016. Simultaneous inoculation of yeasts and lactic acid bacteria: Effects on fermentation dynamics and chemical composition of Negroamaro wine. LWT-Food Science and Technology, 66, 406–412.
Veiga A, Madeira-Lopes A. 2000. Effects of weak acid preservatives on the growth and thermal death of the yeast Pichia membranifaciens in a commercial apple juice. International Journal of Food Microbiology, 56, 145–151.
Wang C X, Liu Y L. 2013. Dynamic study of yeast species and Saccharomyces cerevisiae strains during the spontaneous fermentations of Muscat blanc in Jingyang, China. Food Microbiology, 33, 172–177.
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