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Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2513-2524    DOI: 10.1016/S2095-3119(17)61693-9
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Antimicrobial activity from Piper sarmentosum Roxb. against rice pathogenic bacteria and fungi
Pragatsawat Chanprapai1, 2, Warinthorn Chavasiri2
1 Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
2 Natural Products Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
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Abstract  In vitro antimicrobial activity of the dichloromethane and methanol extracts of Piper sarmentosum leaves, fruits, stems, and the essential oil obtained from the fresh leaves together with their constituents were investigated against two rice pathogenic fungi: Rhizoctonia solani (sheath blight causal agent) and Bipolaris oryzae (brown spot causal agent), and two rice pathogenic bacteria: Xanthomonas oryzae pv. oryzae (Xoo) (bacterial leaf blight causal agent) and pv. oryzicola (Xoc) (bacterial leaf streak causal agent).  Among them, the dichloromethane extracts of the leaves and fruits, and the essential oil showed significantly high potential anti-rice microbial activity.  Based on bioassay-guided fractionation of the dichloromethane leave and fruit extracts, myristicin, sarmentine, brachystamide B, brachyamide B, and piperonal were isolated.  Moreover, the major constituent of its oil was also myristicin.  Myristicin and brachyamide B revealed the highest potent inhibition against R. solani and B. oryzae (half maximal inhibitory concentration (IC50) of 0.69 and 0.12 mmol L–1), respectively.  Moreover, brachyamide B and piperonal displayed most antibacterial activity against Xoo (MIC/MBC 7.62/1.90 mmol L–1) and Xoc (MIC/MBC 2.59/20.75 mmol L–1), respectively.  Additionally, the essential oil also exhibited the antimicrobial activity against all tested rice pathogenic bacteria and fungi.  These compounds and the oil were first evaluated for anti-rice pathogenic microbial activity.
Keywords:  Piper sarmentosum Roxb        bioassay guided fractionation        antimicrobial activity  
Received: 13 September 2016   Accepted:

The research was supported by a grant from the 90th Anniversary of Chulalongkorn University Fund, Thailand (Ratchadaphiseksomphot Endowment Fund, F31GSES13).

Corresponding Authors:  Correspondence Warinthorn Chavasiri, Tel: +66-2-2187625, E-mail:   
About author:  Pragatsawat Chanprapai, E-mail:

Cite this article: 

Pragatsawat Chanprapai, Warinthorn Chavasiri. 2017. Antimicrobial activity from Piper sarmentosum Roxb. against rice pathogenic bacteria and fungi. Journal of Integrative Agriculture, 16(11): 2513-2524.

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