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| YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development |
| WANG Zhong-wei*, ZHANG Tian-quan*, XING Ya-di, ZENG Xiao-qin, WANG Ling, LIU Zhong-xian, SHI Jun-qiong, ZHU Xiao-yan, MA Ling, LI Yun-feng, LING Ying-hua, SANG Xian-chun, HE Guang-hua |
| Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.China |
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Abstract The nuclear-encoded light-harvesting chlorophyll a/b-binding proteins (LHCPs) are specifically translocated from the stroma into the thylakoid membrane through the chloroplast signal recognition particle (cpSRP) pathway. The cpSRP is composed of a cpSRP43 protein and a cpSRP54 protein, and it forms a soluble transit complex with LHCP in the chloroplast stroma. Here, we identified the YGL9 gene that is predicted to encode the probable rice cpSRP43 protein from a rice yellow-green leaf mutant. A phylogenetic tree showed that an important conserved protein family, cpSRP43, is present in almost all green photosynthetic organisms such as higher plants and green algae. Sequence analysis showed that YGL9 comprises a chloroplast transit peptide, three chromodomains and four ankyrin repeats, and the chromodomains and ankyrin repeats are probably involved in protein-protein interactions. Subcellular localization showed that YGL9 is localized in the chloroplast. Expression pattern analysis indicated that YGL9 is mainly expressed in green leaf sheaths and leaves. Quantitative real-time PCR analysis showed that the expression levels of genes associated with pigment metabolism, chloroplast development and photosynthesis were distinctly affected in the ygl9 mutant. These results indicated that YGL9 is possibly involved in pigment metabolism, chloroplast development and photosynthesis in rice.
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Received: 07 December 2015
Accepted:
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| Fund: This research was supported by the Special Fund for Industry of Ministry of Agriculture of China (201303129), the Fundamental Research Funds for the Central Universities, China (XDJK2013A023), the Key Program of Chongqing, China (cstc2012ggC80002), and the Upgrade Project of the Key Laboratory of Chongqing, China (cstc2014pt-sy80001). |
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Corresponding Authors:
HE Guang-hua, Tel: +86-23-68250158, E-mail: heghswu@163.com
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| About author: * These authors contributed equally to this study. |
Cite this article:
WANG Zhong-wei, ZHANG Tian-quan, XING Ya-di, ZENG Xiao-qin, WANG Ling, LIU Zhong-xian, SHI Jun-qiong, ZHU Xiao-yan, MA Ling, LI Yun-feng, LING Ying-hua, SANG Xian-chun, HE Guang-hua.
2016.
YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development. Journal of Integrative Agriculture, 15(05): 944-953.
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| Amin P, Sy D A C, Pilgrim M L, Parry D H, Nussaume L, Hoffman N E. 1999. Arabidopsis mutants lacking the 43-and 54-kilodalton subunits of the chloroplast signal recognition particle have distinct phenotypes. Plant Physiology, 121, 61–70.Armbruster U, Pesaresi P, Pribil M, Hertle A, Leister D. 2011. Update on chloroplast research: New tools, new topics, and new trends. Molecular Plant, 4, 1–16.Bals T, Dunschede B, Funke S, Schunemann D. 2010. Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: An in vivo and in vitro study. FEBS Letters, 584, 4138–4144.Chen S, Tao L, Zeng L, Vega-Sanchez M E, Umemura K, Wang G L. 2006. A highly efficient transient protoplast system for analyzing defence gene expression and protein-protein interactions in rice. Molecular Plant Pathology, 7, 417–427.Falk S, Ravaud S, Koch J, Sinning I. 2010. The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membrane. Journal of Biological Chemistry, 285, 5954–5962.Falk S, Sinning I. 2010. cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a/b-binding proteins. Journal of Biological Chemistry, 285, 21655–21661.Goforth R L, Peterson E C, Yuan J G, Moore M J, Kight A D, Lohse M B, Sakon J, Henry R L. 2004. Regulation of the GTPase cycle in post-translational signal recognition particle-based protein targeting involves cpSRP43. Journal of Biological Chemistry, 279, 43077–43084.Hermkes R, Funke S, Richter C, Kuhlmann J, Schunemann D. 2006. The α-helix of the second chromodomain of the 43 kDa subunit of the chloroplast signal recognition particle facilitates binding to the 54 kDa subunit. FEBS Letters, 580, 3107–3111.Hiei Y, Komari T. 2008. Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. Nature Protocols, 3, 824–834.Horn A, Hennig J, Ahmed Y L, Stier G, Wild K, Sattler M, Sinning I. 2015. Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction. Nature Communications, 6, 8875.Jaru-Ampornpan P, Shen K A, Lam V Q, Ali M, Doniach S, Jia T Z, Shan S O. 2010. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature Structural & Molecular Biology, 17, 696–702.Jarvis P, Robinson C. 2004. Mechanisms of protein import and routing in chloroplasts. Current Biology, 14, R1064-R1077.Jarvis P, Soll J. 2002. Toc, Tic, and chloroplast protein import. Biochimica et Biophysica Acta-molecular Cell Research, 1590, 177–189.Jonas-Straube E, Hutin C, Hoffman N E, Schunemann D. 2001. Functional analysis of the protein-interacting domains of chloroplast SRP43. Journal of Biological Chemistry, 276, 24654–24660.Keegstra K, Cline K. 1999. Protein import and routing systems of chloroplasts. The Plant Cell, 11, 557–570.Klimyuk V I, Persello-Cartieaux F, Havaux M, Contard-David P, Schuenemann D, Meiherhoff K, Gouet P, Jones J D G, Hoffman N E, Nussaume L. 1999. A chromodomain protein encoded by the Arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. The Plant Cell, 11, 87–99.Lewis N E, Marty N J, Kathir K M, Rajalingam D, Kight A D, Daily A, Kumar T K S, Henry R L, Goforth R L. 2010. A dynamic cpSRP43-Albino3 interaction mediates translocase regulation of chloroplast signal recognition particle (cpSRP)-targeting components. Journal of Biological Chemistry, 285, 34220–34230.Li H M, Chiu C C. 2010. Protein transport into chloroplasts. Annual Review of Plant Biology, 61, 157–180.Ouyang M, Li X, Ma J, Chi W, Xiao J, Zou M, Chen F, Lu C, Zhang L. 2011. LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway. Nature Communications, 2, 277.Plumley F G, Schmidt G W. 1995. Light-harvesting chlorophyll a/b complexes: Interdependent pigment synthesis and protein assembly. The Plant Cell, 7, 689–704.Race H L, Herrmann R G, Martin W. 1999. Why have organelles retained genomes? Trends in Genetics, 15, 364–370.Richter C V, Bals T, Schunemann D. 2010. Component interactions, regulation and mechanisms of chloroplast signal recognition particle-dependent protein transport. European Journal of Cell Biology, 89, 965–973.Robinson C, Thompson S J, Woolhead C. 2001. Multiple pathways used for the targeting of thylakoid proteins in chloroplasts. Traffic, 2, 245–251.Schnell D J, Blobel G, Keegstra K, Kessler F, Ko K, Soll J. 1997. A consensus nomenclature for the protein-import components of the chloroplast envelope. Trends in Cell Biology, 7, 303–304.Schunemann D. 2004. Structure and function of the chloroplast signal recognition particle. Current Genetics, 44, 295–304.Schuenemann D, Gupta S, Persello-Cartieaux F, Klimyuk V I, Jones J D G, Nussaume L, Hoffman N E. 1998. A novel signal recognition particle targets light-harvesting proteins to the thylakoid membranes. Proceedings of the National Academy of Sciences of the United States of America, 95, 10312–10316.Sivaraja V, Kumar T K S, Leena P S T, Chang A N, Vidya C, Goforth R L, Rajalingam D, Arvind K, Ye J L, Chou J, Henry R, Yu C. 2005. Three-dimensional solution structures of the chromodomains of cpSRP43. Journal of Biological Chemistry, 280, 41465–41471.Stengel K F, Holdermann I, Cain P, Robinson C, Wild K, Sinning I. 2008. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43. Science, 321, 253–256.Stoebe B, Martin W, Kowallik K V. 1998. Distribution and nomenclature of protein-coding genes in 12 sequenced chloroplast genomes. Plant Molecular Biology Reporter, 16, 243–255.Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.Tu C J, Peterson E C, Henry R, Hoffman N E. 2000. The L18 domain of light-harvesting chlorophyll proteins binds to chloroplast signal recognition particle 43. Journal of Biological Chemistry, 275, 13187–13190.Tzvetkova-Chevolleau T, Hutin C, Noel L D, Goforth R, Carde J P, Caffarri S, Sinning I, Groves M, Teulon J M, Hoffman N E, Henry R, Havaux M, Nussaume L. 2007. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. The Plant Cell, 19, 1635–1648.Umate P. 2010. Genome-wide analysis of the family of light-harvesting chlorophyll a/b-binding proteins in Arabidopsis and rice. Plant Signaling & Behavior, 5, 1537–1542.Wang J L, Jiang J D, Oard J H. 2000. Structure, expression and promoter activity of two polyubiquitin genes from rice (Oryza sativa L.). Plant Science, 156, 201–211.Wellburn A R. 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144, 307–313.Yu B Y, Gruber M Y, Khachatourians G G, Zhou R, Epp D J, Hegedus D D, Parkin I A P, Welsch R, Hannoufa A. 2012. Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus. Journal of Experimental Botany, 63, 5189–5202.Zhang F T, Luo X D, Hu B L, Wan Y, Xie J K. 2013. YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice. Rice, 6, 7.Zhang T Q, Guo S, Xing Y D, Du D, Sang X C, Ling Y H, He G H. 2015. Molecular mapping of a new yellow-green leaf gene YGL9 in rice (Oryza sativa L.). Acta Agronomica Sinica, 41, 989–997. (in Chinese) |
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