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Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2266-2278    DOI: 10.1016/S2095-3119(15)61280-1
Physiology·Biochemistry·Cultivation·Tillage Advanced Online Publication | Current Issue | Archive | Adv Search |
Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings
MA Qiao-li1, 3*, KANG Jun-mei2*, LONG Rui-cai2, CUI Yan-jun2, ZHANG Tie-jun2, XIONG Jun-bo2, 4, YANG Qing-chuan2, SUN Yan1
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China
2 Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 School of Chemistry & Chemical Engineering, Ningxia University, Yinchuan 750021, P.R.China
4 Institute of Animal Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China
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Abstract      Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L–1 NaCl and 180 g L–1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.
Keywords:  alfalfa        salinity stress        osmotic stress        seedling growth        proteomics  
Received: 25 September 2015   Accepted: 01 October 2016
Corresponding Authors:  SUN Yan, Tel: +86-10-62732793, E-mail:   
About author:  MA Qiao-li, E-mail:; KANG Jun-mei, E-mail:;

Cite this article: 

MA Qiao-li, KANG Jun-mei, LONG Rui-cai, CUI Yan-jun, ZHANG Tie-jun, XIONG Jun-bo, YANG Qingchuan, SUN Yan. 2016. Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings. Journal of Integrative Agriculture, 15(10): 2266-2278.

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