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| Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| MA Qiao-li1, 3*, KANG Jun-mei2*, LONG Rui-cai2, CUI Yan-jun2, ZHANG Tie-jun2, XIONG Jun-bo2, 4, YANG Qing-chuan2, SUN Yan1 |
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China
2 Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 School of Chemistry & Chemical Engineering, Ningxia University, Yinchuan 750021, P.R.China
4 Institute of Animal Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China |
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Abstract Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L–1 NaCl and 180 g L–1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.
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Received: 25 September 2015
Accepted:
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Corresponding Authors:
SUN Yan, Tel: +86-10-62732793, E-mail: cts-china@cau.edu.cn
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| About author: MA Qiao-li, E-mail: mql_2008@126.com; KANG Jun-mei,
E-mail: kangjmei@126.com; |
Cite this article:
MA Qiao-li, KANG Jun-mei, LONG Rui-cai, CUI Yan-jun, ZHANG Tie-jun, XIONG Jun-bo, YANG Qingchuan, SUN Yan.
2016.
Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings. Journal of Integrative Agriculture, 15(10): 2266-2278.
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| Aghaei K, Ehsanpour A A, Komatsu S. 2008. Proteome analysis of potato under salt stress. Journal of Proteome Research, 7, 4858–4868.Alkhalfioui F, Renard M, Vensel W H, Wong J, Tanaka C K, Hurkman W J, Buchanan B B, Montrichard F. 2007. Thioredoxin-linked proteins are reduced during germination of Medicago truncatula seeds. Plant Physiology, 144, 1559–1579.Cánovas F M, Dumas-Gaudot E, Recorbet G, Jorrin J, Mock H P, Rossignol M. 2004. Plant proteome analysis. Proteomics, 4, 285–298.Daher Z, Recorbet G, Valot B, Robert F, Balliau T, Potin S, Schoefs B, Dumas-Gaudot E. 2010. Proteomic analysis of Medicago truncatula root plastids. Proteomics, 10, 2123–2137.Dvorakova-Hola K, Matuskova A, Kubala M, Otyepka M, Kucera T, Vecer J, Herman P, Parkhomenko N, Kutejova E, Janata J. 2010. Glycine-rich loop of mitochondrial processing peptidase alpha-subunit is responsible for substrate recognition by a mechanism analogous to mitochondrial receptor Tom20. Journal of Molecular Biology, 396, 1197–1210.Frydman J, Nimmesgern E, Ohtsuka K, Hartl F U. 1994. Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones. Nature, 370, 111–117.Fuchs T M, Deppisch H, Scarlato V, Gross R. 1996. A new gene locus of Bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins. Journal of Bacteriology, 178, 4445–4452.Fulda S, Mikkat S, Stegmann H, Horn R. 2011. Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.). Plant Biology, 13, 632–642.Galati D, Srinivasan S, Raza H, Prabu S K, Hardy M, Chandran K, Lopez M, Kalyanaraman B, Avadhani N G. 2009. Role of nuclear-encoded subunit Vb in the assembly and stability of cytochrome c oxidase complex: implications in mitochondrial dysfunction and ROS production. Biochemical Journal, 420, 439–449.Gallardo K, Firnhaber C, Zuber H, Hericher D, Belghazi M, Henry C, Kuster H, Thompson R. 2007. A combined proteome and transcriptome analysis of developing Medicago truncatula seeds. Molecular & Cellular Proteomics, 6, 2165–2179.Genschik P, Jamet E, Philipps G, Parmentier Y, Gigot C, Fleck J. 1994. Molecular characterization of a beta-type proteasome subunit from Arabidopsis thaliana co-expressed at a high level with an alpha-type proteasome subunit early in the cell cycle. The Plant Journal, 6, 537–546.Hajheidari M, Abdollahian-Noghabi M, Askari H, Heidari M, Sadeghian S Y, Ober E S, Salekdeh G H. 2005. Proteome analysis of sugar beet leaves under drought stress. Proteomics, 5, 950–960.Hashimoto M, Komatsu S. 2007. Proteomic analysis of rice seedlings during cold stress. Proteomics, 7, 1293–1302.He X, Thornton J, Carmicle-Davis S, McDaniel L S. 2006. Tex, a putative transcriptional accessory factor, is involved in pathogen fitness in Streptococcus pneumoniae. Microbial Pathogenesis, 41, 199–206.Hebbelmann I, Selinski J, Wehmeyer C, Goss T, Voss I, Mulo P, Kangasjarvi S, Aro E M, Oelze M L, Dietz K J, Nunes-Nesi A, Do P T, Fernie A R, Talla S K, Raghavendra A S, Linke V, Scheibe R. 2012. Multiple strategies to prevent oxidative stress in Arabidopsis plants lacking the malate valve enzyme NADP-malate dehydrogenase. Journal of Experimental Botany, 63, 1445–1459.Houston N L, Fan C Z, Xiang Q Y, Schulze J M, Jung R, Boston R S. 2005. Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins. Plant Physiology, 137, 762–778.Imin N, Nizamidin M, Daniher D, Nolan K E, Rose R J, Rolfe B G. 2005. Proteomic analysis of somatic embryogenesis in Medicago truncatula. Explant cultures grown under 6-benzylaminopurine and 1-naphthaleneacetic acid treatments. Plant Physiology, 137, 1250–1260.Irsigler A S, Costa M D, Zhang P, Reis P A, Dewey R E, Boston R S, Fontes E P. 2007. Expression profiling on soybean leaves reveals integration of ER- and osmotic-stress pathways. BMC Genomics, 8, 431.Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. 2011. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nature Structural & Molecular Biology, 18, 191–254.Johnson S J, Close D, Robinson H, Vallet-Gely I, Dove S L, Hill C P. 2008. Crystal structure and RNA binding of the Tex protein from Pseudomonas aeruginosa. Journal of Molecular Biology, 377, 1460–1473.de Jong F, Mathesius U, Imin N, Rolfe B G. 2007. A proteome study of the proliferation of cultured Medicago truncatula protoplasts. Proteomics, 7, 722–736.Jordan D B, Chollet R. 1983. Inhibition of ribulose bisphosphate carboxylase by substrate ribulose 1,5-bisphosphate. Journal of Biological Chemistry, 258, 13752–13758.Kerepesi I, Galiba G. 2000. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science, 40, 482–487.Komatsu S, Deschamps T, Hiraga S, Kato M, Chiba M, Hashiguchi A, Tougou M, Shimamura S, Yasue H. 2012. Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots. Plant Molecular Biology, 78, 197–197.Komatsu S, Tanaka N. 2005. Rice proteome analysis: A step toward functional analysis of the rice genome. Proteomics, 5, 938–949.Krasensky J, Jonak C. 2012. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany, 63, 1593–1608.Li B, He L Z, Guo S R, Li J, Yang Y J, Yan B, Sun J, Li J. 2013. Proteomics reveal cucumber Spd-responses under normal condition and salt stress. Plant Physiology and Biochemistry, 67, 7–14.Liska A J, Shevchenko A, Pick U, Katz A. 2004. Enhanced photosynthesis and redox energy production contribute to salinity tolerance in Dunaliella as revealed by homology-based proteomics. Plant Physiology, 136, 2806–2817.Liu Y, Zhu J B, Yu G Q, Zou H S. 2010. The enoyl-ACP reductase gene, fabI1, of Sinorhizobium meliloti is involved in salt tolerance, swarming mobility and nodulation efficiency. Chinese Science Bulletin, 55, 259–262.Long R C, Yang Q C, Kang J M, Zhang T J, Wang H M, Li M N, Zhang Z. 2013. Overexpression of a novel salt stress-induced glycine-rich protein gene from alfalfa causes salt and ABA sensitivity in Arabidopsis. Plant Cell Reports, 32, 1289–1298.Ma H Y, Song L R, Shu Y J, Wang S, Niu J, Wang Z K, Yu T, Gu W H, Ma H. 2012. Comparative proteomic analysis of seedling leaves of different salt tolerant soybean genotypes. Journal of Proteomics, 75, 1529–1546.Manaa A, Ben Ahmed H, Valot B, Bouchet J P, Aschi-Smiti S, Causse M, Faurobert M. 2011. Salt and genotype impact on plant physiology and root proteome variations in tomato. Journal of Experimental Botany, 62, 2797–2813.Munns R, Husain S, Rivelli A R, James R A, Condon A G, Lindsay M P, Lagudah E S, Schachtman D P, Hare R A. 2002. Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant and Soil, 247, 93–105.Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681.Ngara R, Ndimba R, Borch-Jensen J, Jensen O N, Ndimba B. 2012. Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings. Journal of Proteomics, 75, 4139–4150.Nozu Y, Tsugita A, Kamijo K. 2006. Proteomic analysis of rice leaf, stem and root tissues during growth course. Proteomics, 6, 3665–3670.Ohana B, Moore P A, Ruben S M, Southgate C D, Green M R, Rosen C. 1993. The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes. Proceedings of the National Academy of Sciences of the United States of America, 90, 138–142.Ohtsuka J, Ichihara Y, Ebihara A, Nagata K, Tanokura M. 2009. Crystal structure of TTHA1264, a putative M16-family zinc peptidase from Thermus thermophilus HB8 that is homologous to the beta subunit of mitochondrial processing peptidase. Proteins, 75, 774–780.Orlowski M. 1990. The multicatalytic proteinase complex, a major extralysosomal proteolytic system. Biochemistry, 29, 10289–10297.Parida A K, Das A B. 2005. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60, 324–349.Parry M A J, Andralojc P J, Mitchell R A C, Madgwick P J, Keys A J. 2003. Manipulation of Rubisco: The amount, activity, function and regulation. Journal of Experimental Botany, 54, 1321–1333.Satoh T, Ishizuka T, Tomaru T, Yoshino S, Nakajima Y, Hashimoto K, Shibusawa N, Monden T, Yamada M, Mori M. 2009. Tat-binding protein-1 (TBP-1), an ATPase of 19S regulatory particles of the 26S proteasome, enhances androgen receptor function in cooperation with TBP-1-interacting protein/Hop2. Endocrinology, 150, 3283–3290.Sengupta S, Majumder A L. 2009. Insight into the salt tolerance factors of a wild halophytic rice, Porteresia coarctata: a physiological and proteomic approach. Planta, 229, 911–929.Sepe M, Festa L, Tolino F, Bellucci L, Sisto L, Alfano D, Ragno P, Calabro V, de Franciscis V, La Mantia G, Pollice A. 2011. A regulatory mechanism involving TBP-1/Tat-binding protein 1 and Akt/PKB in the control of cell proliferation. PLoS One, 6, e22800. Seufert W, Jentsch S. 1990. Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. EMBO Journal, 9, 543–550.Soares N C, Francisco R, Ricardo C P, Jackson P A. 2007. Proteomics of ionically bound and soluble extracellular proteins in Medicago truncatula leaves. Proteomics, 7, 2070–2082.Surolia N, Surolia A. 2001. Triclosan offers protection against blood stages of malaria by inhibiting enoyl-ACP reductase of Plasmodium falciparum. Nature Medicine, 7, 636–636.Swami A K, Alam S I, Sengupta N, Sarin R. 2011. Differential proteomic analysis of salt stress response in Sorghum bicolor leaves. Environmental and Experimental Botany, 71, 321–328.Tang L L, Cai H, Ji W, Luo X, Wang Z Y, Wu J, Wang X D, Cui L, Wang Y, Zhu Y M, Bai X. 2013. Overexpression of GsZFP1 enhances salt and drought tolerance in transgenic alfalfa (Medicago sativa L.). Plant Physiology and Biochemistry, 71, 22–30.Taylor C T, Moncada S. 2010. Nitric oxide, cytochrome c oxidase, and the cellular response to hypoxia. Arteriosclerosis Thrombosis and Vascular Biology, 30, 643–647.Thomas J D, VanBogelen R A. 2000. Experimentalism in proteomics. Trends in Biotechnology, 18, 7–11.Tu Y, Rochfort S, Liu Z Q, Ran Y D, Griffith M, Badenhorst P, Louie G V, Bowman M E, Smith K F, Noel J P, Mouradov A, Spangenberg G. 2010. Functional analyses of caffeic acid O-methyltransferase and cinnamoyl-CoA-reductase genes from perennial ryegrass (Lolium perenne). The Plant Cell, 22, 3357–3373.Umate P. 2010. Genome-wide analysis of thioredoxin fold superfamily peroxiredoxins in Arabidopsis and rice. Plant Signaling Behavior, 5, 1543–1546.Wang W B, Kim Y H, Lee H S, Kim K Y, Deng X P, Kwak S S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry, 47, 570–577.Wang W X, Vinocur B, Shoseyov O, Altman A. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science, 9, 244–252.Wang X Q, Yang P F, Gao Q, Liu X L, Kuang T Y, Shen S H, He Y K. 2008. Proteomic analysis of the response to high-salinity stress in Physcomitrella patens. Planta, 228, 167–177.Watanabe S, Kojima K, Ide Y, Sasaki S. 2000. Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tissue and Organ Culture, 63, 199–206.Xiong J B, Yang Q C, Kang J M, Sun Y, Zhang T J, Margaret G, Ding W. 2011. Simultaneous isolation of DNA, RNA, and protein from Medicago truncatula L. Electrophoresis, 32, 321–330.Zhu J K. 2002. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247–273.Zhu Z, Chen J, Zheng H L. 2012. Physiological and proteomic characterization of salt tolerance in a mangrove plant, Bruguiera gymnorrhiza (L.) Lam. Tree Physiology, 32, 1378–1388.Zwickl P, Grziwa A, Puehler G, Dahlmann B, Lottspeich F, Baumeister W. 1992. Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase. Biochemistry, 31, 964–972. |
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