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Journal of Integrative Agriculture  2018, Vol. 17 Issue (06): 1289-1298    DOI: 10.1016/S2095-3119(17)61821-5
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GmDRR1, a dirigent protein resistant to Phytophthora sojae in Glycine max (L.) Merr.
CHEN Qing-shan1, YU Guo-long1, ZOU Jia-nan1, WANG Jing1, QIU Hong-mei1, ZHU Rong-sheng1, CHANG Hui-lin1, JIANG Hong-wei1, HU Zhen-bang1, LI Chang-yu1, ZHANG Yan-jiao1, WANG Jin-hui1, WANG Xue-ding1, GAO Shan1, LIU Chun-yan1, QI Zhao-ming1, FU Yong-fu2, XIN Da-wei1 
1 Key Laboratory of Soybean Biology, Ministry of Education/College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China
2 Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
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摘要  Received  26 June, 2017    Accepted  17 October, 2017
© 2018 CAAS. Publishing services by Elsevier B.V.  All rights reserved.

Soil-borne pathogen Phytophthora sojae is an oomycete that causes devastating damage to soybean yield.  To mine original resistant genes in soybean is an effective and environmentally-friend approach controlling the disease.  In this study, soybean proteins were extracted from the first trifoliolates infected by predominant P. sojae race 1 and analyzed by two-dimensional gel electrophoresis.  Nineteen differently-expressed protein spots were detected, and 10 of them were further applied for Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry Assay.  One protein containing a dirigent (DIR) domain was identified and belonged to the DIR-b/d family.  Therefore, it was named as GmDRR1 (Glycine max Disease Resistance Response 1).  Then, GmDRR1 gene was pathologically confirmed to be involved in the resistant to P. sojae in soybean.  GmDRR1-GFP (green fluorescent protein) fusion proteins localized in the cell membrane.  qRT-PCR results showed GmDRR1 gene expressed differently in P. sojae resistant- and susceptible-soybean cultivars.  By the promoter analysis, we found a haplotype H8 was existing in most resistant soybean varieties, while a haplotype H77 was existing in most susceptible soybean varieties.  The H77 haplotype had seven SNPs (C to A, G to C, C to A, T to A, T to C, T to C, and T to A) and two single nucleotide insertions.  The results supported that the expression difference of GmDRR1 genes between P. sojae resistant- and susceptible-soybean cultivars might depend on the GmDRR1 promoter SNPs.  The results suggested that GmDRR1 was a dirigent protein involved in soybean resistant to P. sojae and paved a novel way for investigation of the molecular regulatory mechanism of the defense response to P. sojae in soybean.
Keywords:  dirigent domain ')" href="#">  
Received: 26 June 2017   Accepted:
Fund: This study was financially supported by the Academic Skeleton Support Plan of Department of Education of Heilongjiang Province, China (1254G011), the National Natural Science Foundation of China (31271747, 31471516, 31400074, 31401465, 31501332), the National High-Tech R&D Program of China (the 863 Program, 2013AA102602), the Research Fund for the Doctoral Program of Higher Education of China (20122325120015), the Academic Backbone Project of Northeast Agricultural University, China (15XG02), and the Talented Young Project of Northeast Agricultural University, China (518062).
Corresponding Authors:  XIN Da-wei, Tel/Fax: +86-451-55191945,E-mail:dwxin@neau.edu.cn;FU Yong-fu, Tel/Fax: +86-10-82105864,E-mail:fuyongfu@caas.cn; QI Zhao-ming,Tel/Fax:+86-451-55191945,E-mail:qizhaoming1860@126.com   
About author:  CHEN Qing-shan, E-mail: qshchen@126.com;
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CHEN Qing-shan
YU Guo-long
ZOU Jia-nan
QIU Hong-mei
ZHU Rong-sheng
CHANG Hui-lin
JIANG Hong-wei
HU Zhen-bang
LI Chang-yu
ZHANG Yan-jiao
WANG Jin-hui
WANG Xueding
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CHEN Qing-shan, YU Guo-long, ZOU Jia-nan, WANG Jing, QIU Hong-mei, ZHU Rong-sheng, CHANG Hui-lin, JIANG Hong-wei, HU Zhen-bang, LI Chang-yu, ZHANG Yan-jiao, WANG Jin-hui, WANG Xueding, GAO Shan.... 2018. GmDRR1, a dirigent protein resistant to Phytophthora sojae in Glycine max (L.) Merr.. Journal of Integrative Agriculture, 17(06): 1289-1298.

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