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| Assessment of wheat variety distinctness using SSR markers |
| WANG Li-xin1, QIU Jun2, CHANG Li-fang3, LIU Li-hua1, LI Hong-bo1, PANG Bin-shuang1, ZHAO Chang-ping1 |
1、Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences,
Beijing 100097, P.R.China
2、National Agriculture Technology Extension and Service Center, Ministry of Agriculture, Beijing 100026, P.R.China
3、National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of
Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat (SSR) markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value (GSV) ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were >90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV>90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.
Abstract Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat (SSR) markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value (GSV) ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were >90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV>90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.
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Received: 30 September 2014
Accepted:
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| Fund: This study was funded by the Innovation Platform for Basic Research in Breeding of Beijing, China (D08070500690801), and the Science and Technology Innovation Ability Construction Projects, Beijing Academy of Agricultural and Forestry Sciences, China (KJCX20140202). |
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Corresponding Authors:
WANG Li-xin, Tel: +86-10-51503966,Fax: +86-10-51503757, E-mail: wanglx53@263.net;ZHAO Chang-ping, Tel: +86-10-51503968, Fax: +86-10-51503757, E-mail: cp_zhao@vip.sohu.com
E-mail: wanglx53@263.net;ZHAO Chang-ping,cp_zhao@vip.sohu.com
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Cite this article:
WANG Li-xin1, QIU Jun2, CHANG Li-fang3, LIU Li-hua1, LI Hong-bo1, PANG Bin-shuang1, ZHAO Chang-ping1.
2015.
Assessment of wheat variety distinctness using SSR markers. Journal of Integrative Agriculture, 14(10): 1923-1935.
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