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Journal of Integrative Agriculture  2015, Vol. 14 Issue (4): 724-731    DOI: 10.1016/S2095-3119(14)60982-5
Special Issue: 线虫合辑Nematology
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
A new pathotype characterization of Daxing and Huangyuan populations of cereal cyst nematode (Heterodera avenae) in China
 CUI Jiang-kuan, HUANG Wen-kun, PENG Huan, LIU Shi-ming, WANG Gao-feng, KONG Lin-an, PENG De-liang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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摘要  The cereal cyst nematode (CCN, Heteroder aavenae) causes serious yield loss on cereal crops, especially wheat, worldwide. Daxing population in Beijing City and Huangyuan population in Qinghai Province, China, are two CCN populations. In this study, the CCN pathotypes of Daxing and Huangyuan populations were characterized by tests on 23 standard “International Test Assortment” with the local species Wenmai 19 as the susceptible control. Tested materials were grouped by three nematode populations’ virulence on resistant genes (Rha1, Rha2, Rha3, Cre1) and nonresistant genes, varieties and lines. Both Daxing and Huangyuan populations were avirulent to Ortolan (Ha1). Barley cvs. Ortolan, Siri, Morocco, Bajo Aragon 1-1, and Martin 403-2 were all resistant to both populations. Cultivars Herta, Harlan 43 and wheat Iskamish-K-2-light were all susceptible to Huangyuan population, all of them, however, were resistant to Daxing population. The other five oats were all resistant to the two tested CCN populations. Except Iskamisch K-2-light, all the other wheat cultivars (Capa, Loros×Koga, AUS 10894, and Psathias) were susceptible to Daxing population. Because the pathotypes of the two tested CCN populations in Beijing and Qinghai were not identical to any of the 13 pathotypes previously characterized by the test assortment, we classified Daxing and Huangyuan populations as the new pathotypes, named Ha91.

Abstract  The cereal cyst nematode (CCN, Heteroder aavenae) causes serious yield loss on cereal crops, especially wheat, worldwide. Daxing population in Beijing City and Huangyuan population in Qinghai Province, China, are two CCN populations. In this study, the CCN pathotypes of Daxing and Huangyuan populations were characterized by tests on 23 standard “International Test Assortment” with the local species Wenmai 19 as the susceptible control. Tested materials were grouped by three nematode populations’ virulence on resistant genes (Rha1, Rha2, Rha3, Cre1) and nonresistant genes, varieties and lines. Both Daxing and Huangyuan populations were avirulent to Ortolan (Ha1). Barley cvs. Ortolan, Siri, Morocco, Bajo Aragon 1-1, and Martin 403-2 were all resistant to both populations. Cultivars Herta, Harlan 43 and wheat Iskamish-K-2-light were all susceptible to Huangyuan population, all of them, however, were resistant to Daxing population. The other five oats were all resistant to the two tested CCN populations. Except Iskamisch K-2-light, all the other wheat cultivars (Capa, Loros×Koga, AUS 10894, and Psathias) were susceptible to Daxing population. Because the pathotypes of the two tested CCN populations in Beijing and Qinghai were not identical to any of the 13 pathotypes previously characterized by the test assortment, we classified Daxing and Huangyuan populations as the new pathotypes, named Ha91.
Keywords:  cereal cyst nematode       Heterodera avenae       Daxing population       Huangyuan population       pathotype  
Received: 01 April 2014   Accepted:
Fund: 

This study was financially supported by the National Natural Science Foundation of China (31171827) and the National Key Basic Research Program of China (973 Program, 2013CB127502).

Corresponding Authors:  PENG De-liang, Tel: +86-10-62815611,E-mail: pengdeliang@caas.cn     E-mail:  pengdeliang@caas.cn
About author:  CUI Jiang-kuan, E-mail: jk_cui@163.com;

Cite this article: 

CUI Jiang-kuan, HUANG Wen-kun, PENG Huan, LIU Shi-ming, WANG Gao-feng, KONG Lin-an, PENG De-liang. 2015. A new pathotype characterization of Daxing and Huangyuan populations of cereal cyst nematode (Heterodera avenae) in China. Journal of Integrative Agriculture, 14(4): 724-731.

Andersen S. 1959. Resistance of barley to various populations ofthe cereal root eelworm (Heterodera major). Nematologica,4, 91.

Andersen S. 1961. Resistens mod Havreål Heterodera avenae.Meddr K. Vet. og Landbøhjsk. København, 68, 179. (inDanish)

Andersen S, Andersen K. 1982. Suggestions for determinationand terminology of pathotypes and genes for resistance in cyst-forming nematodes, especially Heterodera avenae.EPPO Bulletin, 12, 379-386

Al-Hazmi A S, Cook R, Ibrahim A A M. 2001. Pathotypecharacterization of the cereal cyst nematode, Heteroderaavenae, in Saudi Arabia. Nematology, 3, 379-382

Van Berkum J A, Seshadri A R. 1970. Some importantnematode problems in India. In: Proceedings of the tenthinternational nematology symposium of the EuropeanSociety of Nematologists. 8-13 September. Pescara, Italy.pp. 136-137

Brown R H. 1969. The occurrence of biotypes of the cereal cystnematode (Heterodera avenae Woll) in Victoria. AustralianJournal of Experimental Agriculture and Animal Husbandry,9, 453-456

Chen P S, Peng D L, Wang M Z. 1992. Cereal cyst nematodedisease of wheat. Plant Protection, 18, 37-38 (in Chinese)

Cook R. 1982. Cereal and grass hosts of some gramineouscyst nematodes. EPPO Bulletin, 12, 399-411

Cook R, Noel G R. 2002. Cyst nematodes: Globodera andHeterodera species. In: Starr J L, Cook R, Bridge J, eds.,Plant Resistance to Parasitic Nematodes. CABI Publishing,Wallingford, UK. pp. 71-105

Cook R, Rivoal R. 1998. Genetics of resistance andparasitism. In: Sharma S B, ed., The Cyst Nematodes.Chapman and Hall, London, UK. pp. 322-352

Hou S Y, Wang A L, Zhang G, Huang L L. 2012. Effect of cerealcyst nematode on agronomic characteristics and yieldlosses of spring wheat. Journal of Agriculture, 2, 33-36(in Chinese)

Kretschmer J M, Chalmers K J, Manning S, Karakousis A, BarrA R, Islam A K M R, Logue S J, Choe Y W, Barker S J,Lance R C M, Langridge P. 1997. RFLP mapping of the Ha2cereal cyst nematode resistance gene in barley. Theoreticaland Applied Genetics, 94, 1060-1064

Li H M, Wang X, Pei S, Le X H, Liang Z. 2010. A preliminarysurvey of the cereal cyst nematode Heterodera avenae onwheat in Jiangsu Province of China. Plant Protection, 36,172-175 (in Chinese)

Li H X, Liu Y E, Wei Z, Li M Q. 2012. The detection of Heteroderaavenae from the cereal field in Autonomous Regions of Tibetand Xinjiang. Nematology Research in China, 4, 164-165(in Chinese)

Liu J, Wu H Y, Zhang G M, Yu G L, Peng D L. 2009.Observations and morphometrics of cereal cyst nematodefrom Binzhou, Shandong, China. In: Proceedings of theFirst Workshop of the International Cereal Cyst NematodeInitiative. 21-23 October, 2009 Antalya, Turkey.

Mathur B N, Arya H C. 1974. The occurrence of biotypes of thecereal cyst nematode (Heterodera avenae) in the light soilsof Rajasthan and Harya, India. Nematology, 20, 19-26.

Nicol J M, Rivoal R 2007. Global knowledge and its applicationfor the integrated control and management of nematodeson wheat. In: Ciancio A, Mukerji K G, eds., IntegratedManagement and Biocontrol of Vegetable and Grain CropNematodes. Springer, Dordrecht, The Netherlands. pp.251-294

Nicol J M, Rivoal R. 2008. Global knowledge and its applicationfor the integrated control and management of nematodeson wheat. In: Ciancio A, Mukerji K G, eds., Integratedmanagement and Biocontrol of Vegetable and GrainCrops Nematodes. Springer Academic Publishing, TheNetherlands. pp. 243-287

Nicol J M. 2002. Important nematode pests. In: Curtis BC, Rajaram S, Macpherson H G, eds., Bread Wheat:Improvementand Production. Food and AgricultureOrganization of the United Nations, Rome, Italy. pp.345-366

Peng D L, Cook R. 1996. Observation on pathotypes of cerealcyst nematode (Heterodera avenae) in China. Afro-AsianJournal of Nematology, 6, 176-178

Peng D L, Huang W K, Sun J H, Liu C J, Zhang H M. 2012.First report of cereal cyst nematode (Heterodera avenae) inTianjin, China. Nematology Research in China, 4, 162-163(in Chinese)

Peng D L, Li H X, Wang X F, Riley I T, Nicol J M, Hou S Y,Ou S Q. 2008. New distribution of cereal cyst nematode(Heterodera avenae) in China. Nematology Research inChina, 2, 344-345 (in Chinese)

Peng D L, Nicol J M, Li H M, Hou S Y, Li H X, Chen S L, MaP, Li H L, Riley I T. 2009. Current knowledge of cereal cystnematode (Heterodera avenae ) on wheat in China. In: RileyI T, Nicol J M, Dababat A A, eds., Cereal Cyst Nematodes:Status, Research and Outlook. CIMMYT, Ankara, Turkey.pp. 29-34

Peng D L, Subbotin S, Moens M. 2003. rDNA restrictionfragment length polymorphism of Heterodera avenae inChina. Acta Phytopathologica Sinica, 33, 323-329 (inChinese)

Peng D L, Zhang D, Nicol J M, Chen S L, Waeyenberge L,Moens M, Li H L,Tang W H, Riley I T. 2007. Occurrence,distribution and research situation of cereal cyst nematodein China. In: Proceedings of the XVI International PlantProtection Congress. 15-18 October, 2007 Glasgow,Scotland, UK. British Crop Protection Council, Alton,Hampshire, UK. pp. 350-351

Peng D L, Zhang D S, Qi S H, Chen P S. 1995. Distributionand control of cereal cyst nematode (Heterodera avenae)in China. In: Advances of Chinese Plant Protection. ChinaScience and Technology Press, China. pp. 53-56 (inChinese)

Persmark L, Banck A, Andersson S. 1992. Evaluation ofmethods for extraction of nematodes and endoparasiticfungi from soil. Nematologica, 38, 520-530

Person-Dedryver F, Doussinault G. 1984. Genetic interactionsbetween French pathotypes of Heterodera avenae Woll.and barley varieties. I. Varietal behavior. Agronomie, 4,763-771 (in French)

Qi X L, Peng D L, Peng H, Long H B, Huang W K, He W T. 2012.Rapid molecular diagnosis based on SCAR marker systemfor cereal cyst nematode. Scientia Agricultura Sinica, 45,4388-4395 (in Chinese)

Riley I T, McKay A C. 2009. Cereal cyst nematode in Australia: Biography of a biological invader In: Rile I T, Nicol J M,Dababat A A, eds., Cereal Cyst Nematodes: Status,Research and Outlook. CIMMYT, Ankara, Turkey. pp.23-28

Rivoal R, Cook R. 1993. Nematode pests of cereals. In:Evans K, Trudgill D L, Webster J M, eds., Plant ParasiticNematodes in Temperate Agriculture. CAB International,Wallingford, UK. pp. 259-303

Sanchez A, Zancada C. 1987. Characterization of Heteroderaavenae pathotypes from Spain. Nematologica, 33, 55-60

Smiley R W, Gui P Y, John N P. 2011. Resistance of wheat,barley and oat to Heterodera avenae in the PacificNorthwest, USA. Nematology, 13, 539-552

Smiley R W, Nicol J M. 2009. Nematodes which challengeglobal wheat production. In: Carver B F, ed., Wheat Scienceand Trade. Wiley-Blackwell, Ames I A, USA. pp. 171-187

Smiley R W, Whittaker R G, Gourlie J A, Easley S A, InghamR E. 2005. Plant-parasitic nematodes associated withreduced wheat yield in Oregon: Heterodera avenae. Journalof Nematology, 37, 297-307

Valerie M W. 1999. Plant nematode resistance genes. CurrentOpinion in Plant Biology, 2, 327-331

Yuan H X, Sun J W, Yang W X, Xing X P, Wang Z Y, RileyI T, Li H L. 2010. New pathotypes of Heterodera avenae(cereal cyst nematode) from winter wheat in Zhengzhou,Henan, China. Australasian Plant Pathology, 39, 107-111

Zheng J W, Cheng H R, Fang Z D. 1997. Pathotype of cerealcyst nematodes (Heterodera avenae) on wheat in Shanxiand Anhui, China. Acta Phyto Pathologica Sinica, 27,309-314 (in Chinese)

Zheng J W, Lin M, Cheng H, Fang Z. 1996. Identifiction of twocyst nematode populations parasitizing wheat in Anhui,China. Jiangsu Journal of Agricultural Sciences, 12, 31-35(in Chinese)
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