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| Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| ZENG Qiu-feng, YANG Xia, ZHENG Ping, ZHANG Ke-ying, LUO Yu-heng, DING Xue-mei, BAI Shi-ping, WANG Jian-ping, XUAN Yue, SU Zhuo-wei |
| Key Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University,Ya’an 625014, P.R.China |
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摘要 The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg–1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg–1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.
Abstract The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg–1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg–1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.
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Received: 07 November 2014
Accepted:
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| Fund: This research was supported by grants from the National Natural Science Foundation of China (31101733). |
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Corresponding Authors:
ZENG Qiu-feng, Mobile: +86-13778765040,Fax: +86-835-2885065, E-mail: zqf@sicau.edu.cn
E-mail: zqf@sicau.edu.cn
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| About author: These authors contributed equally to this study |
Cite this article:
ZENG Qiu-feng, YANG Xia, ZHENG Ping, ZHANG Ke-ying, LUO Yu-heng, DING Xue-mei, BAI Shi-ping, WANG Jian-ping, XUAN Yue, SU Zhuo-wei.
2016.
Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers. Journal of Integrative Agriculture, 15(1): 183-190.
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| Bottje W G, Wideman R F. 1995. Potential role of free radicalsin the pathogenesis of pulmonary hypertension syndrome.Poultry and Avian Biology Reviews, 6, 211-231Carmeliet P, Ferreira V, Breier G, Pollefeyt S, Kieckens L,Gertsenstein M, Fahrig M, Vandenhoeck A, Harpal K,Eberhardt C, Declercq C, Pawling J, Moons L, CollenD, Risau W, Nagy A 1996 Abnormal blood vesseldevelopment and lethality in embryos lacking a single VEGFallele. Nature, 380, 435-439Cort W M. 1974. Antioxidant activity of tocopherols, ascorbylpalmitate, and ascorbic acid and their mode of action.Journal of the American Oil Chemists Society, 51, 321-325Daneshyar M, Kermanshahi H, Golian A. 2009. Changes of biochemical parameters and enzyme activities in broilerchickens with cold-induced ascites. Poultry Science, 88,106-110Enkvetchakul B, Beasley J, Bottje W. 1995. Pulmonary arteriolehypertrophy in broilers with pulmonary hypertensionsyndrome (ascites). Poultry Science, 74, 1677-1682Enkvetchakul B, Bottje W, Anthony N, Moore R, Huff W. 1993.Compromised antioxidant status associated with ascites inbroilers. Poultry Science, 72, 2272-2280Ema M, Taya S, Yokotani N, Sogawa K, Matsuda Y, Fujii-Kuriyama Y. 1997. A novel bHLH-PAS factor with closesequence similarity to hypoxia-inducible factor 1 alpharegulates the VEGF expression and is potentially involved inlung and vascular development. Proceedings of the NationalAcademy of Sciences of the United States of America, 94,4273-4278Elvert G, Kappel A, Heidenreich R, Englmeier U, Lanz S, AckerT, Rauter M, Plate K, Sieweke M, Breier G, Flamme I. 2003.Cooperative interaction of hypoxia-inducible factor-2alpha(HIF-2alpha) and Ets-1 in the transcriptional activationof vascular endothelial growth factor receptor-2 (Flk-1).Journal of Biological Chemistry, 278, 7520-7530Ferrara N, Carver-More K, Chen H, Dowd M, Lu L, O’SheaK S, Powell-Braxton L, Hillan K J, Moore M W. 1996.Heterozygous embryonic lethality induced by targetedinactivation of the VEGF gene. Nature, 380, 439-442Fuh G, Li B, Crowley C, Cunningham B, Wells J A. 1998.Requirements for binding and signaling of the kinase domainreceptor for vascular endothelial growth factor. Journal ofBiological Chemistry, 273, 11197-11204Haddad J J, Land S C. 2001. Non-hypoxic, ROS-sensitivepathway mediates TNF-alpha-dependent regulation ofHIF-Ialpha. FEBS Letters, 505, 269-274José R, Nespereira B, Pérez-Ilzarbe M, Eguinoa E, PáramoJ A. 2005. Vitamins C and E prevent endothelial VEGFand VEGFR-2 overexpression induced by porcinehypercholesterolemic LDL. Cardiovascular Research, 65,665-673Julian R J, McMillan I, Quinton M. 1989. The effect of coldand dietary energy on right ventricular hypertrophy, rightventricular failure and ascites in meat-type chickens. AvianPathology, 18, 675-684Kappel A, Roenicke V, Damert A, Flamme I, Risau W,Breier G. 1999. Identification of VEGF receptor-2 (Flk-1)promoter/enhancer sequences driving endothelium-specifictranscription in transgenic mice. Blood, 93, 4284-4292Kim H N, Kim H, Kong J M, Bae S, Kim Y S, Lee N, Cho B J, LeeS K, Kim H R, Hwang Y I. 2011. Vitamin C down-regulatesVEGF production in B16F10 murine melanoma cells via thesuppression of p42/44 MAPK activation. Journal of CellularBiochemistry, 112, 894-901Knowles H J, Raval R R, Harris A L, Ratcliffe P J. 2003. Effectof ascorbate on the activity of hypoxia inducible factor incancer cells. Cancer Research, 63, 1764-1768Ladmakhi M H, Buys N, Dewil E, Rahimi G, Decuypere E.1997. The prophylactic effect of vitamin C supplementationon broiler ascites incidence and plasma thyroid hormoneconcentration. Avian Pathology, 26, 33-44Livak K J, Schmittgen T D. 2001. Analysis of relative geneexpression data using real-time quantitative PCR and the2(-delta delta C(T)) method. Method, 25, 402-408Morrell N W, Atochina E N, Morris K G, Danilov S M, StenmarkK R. 1995. Angiotensin converting enzyme expression isincreased in small pulmonary arteries of rats with hypoxiainducedpulmonary hypertension. Journal of ClinicalInvestigation, 96, 1823.Mujahid A, Furuse M. 2009. Oxidative damage in differenttissues of neonatal chicks exposed to low environmentaltemperature. Comparative Biochemistry and Physiology(Part A, Molecular and Integrative Physiology), 152,604-608Nespereira B, Pérez-Ilzarbe M, Fernández P, Fuentes A M,Páramo J A, Rodr??guez J A. 2003. Vitamins C and Edownregulate vascular VEGF and VEGFR-2 expressionin apolipoprotein-E-deficient mice. Atherosclerosis, 171,67-73NRC (National Research Council). 1994. Nutrient Requirementsof Poultry. 9th ed. National Academies Press, Washington,D.C.Sahin E, Gumuslu S. 2004. Cold-stress-induced modulation ofantioxidant defence: role of stressed conditions in tissueinjury followed by protein oxidation and lipid peroxidation.International Journal of Biometeorology, 48, 165-171Semenza G L. 2000. Oxygen-regulated transcription factorsand their role in pulmonary disease. Respiratory Research,1, 159-162Steve B, Zähringer C, BelAiba R S, Djordjevic T, Hess D,Michiels C, Kietzmann T, Görlach A. 2007. Reactive oxygenspecies activate the HIF-1α promoter via a functional NF-κB site. Arteriosclerosis Thrombosis and Vascular Biology,27, 755-761Tan X, Liu Y J, Li J C, Pan J Q, Sun W D, Wang X L. 2005.Activation of PKCα and pulmonary vascular remodellingin broilers. Research in Veterinary Science, 79, 131-137Tuder R M, Alard I, Voeikel N F. 1996. Role of vascularendothelial growth factor in hypoxia and monocmtalineinduced pulmonary hypertension. Circulation, 94, 2625-2628Waltenberger J, Mayr U, Pentz S, Hombach V. 1996. Functionalup-regulation of the vascular endothelial growth factorreceptor KDR by hypoxia. Circulation, 94, 1647-1654Wang G L, Jiang B H, Rue E A, Semenza G L. 1995.Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PASheterodimer regulated by cellular O2 tension. Proceedingsof the National Academy of Sciences of the United Statesof America, 92, 5510-5514Wang G L, Semenza G L. 1995. Purification and characterizationof hypoxia-inducible factor 1. Journal of BiologicalChemistry, 270, 1230-1237Wang Y, Guo Y, Dong N, Peng Y, Cai H, Tan J Z, Yang Y, Liu D. 2012. Changes of hepatic biochemical parameters andproteomics in broilers with cold-induced ascites. Journal ofAnimal Science and Biotechnology, 3, 41.Xiang R, Sun W, Wang J, Wang X. 2002. Effect of vitamin C onpulmonary hypertension and muscularisation of pulmonaryarterioles in broilers. British Poultry Science, 43, 705-712Yang X, Luo Y H, Zeng Q F, Zhang K Y, Ding X M, Bai S P,WangJ P. 2014. Effects of low ambient temperatures and dietaryvitamin C supplementation on growth performance, bloodparameters, and antioxidant capacity of 21-day-old broilers.Poultry Science, 93, 898-905Yu A Y, Shimoda L A, Iyer N V, Huso D L, Sun X, McWilliamsR, Beaty T, Sham J S K, Wiener C M, Sylvester J T. 1999.Impaired physiological responses to chronic hypoxia in micepartially deficient for hypoxia-inducible factor 1. Journal ofClinical Investigation, 103, 691–696. |
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