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| Haploid Induction via In vitro Gynogenesis in Tomato (Solanum lycopersicum L.) |
| ZHAO He, WANG Xiao-xuan, DU Yong-chen, ZHU De-wei, GUO Yan-mei, GAO Jian-chang, LI Fei , John C Snyder |
1、Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Department of Horticulture, University of Kentucky, Lexington, KY 40546-0091, USA |
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摘要 In order to determine the potential for haploid induction via in vitro gynogenesis in tomato, the ovules and protoplasts of embryo sacs from the hybrids Zhongza 101 and Zhongza 105 were cultured. An efficient method of ovule isolation was established in this study. Using this method, 100-150 ovules could be isolated from one ovary. Isolated ovules were cultured on three induction media to induce gynogenesis in vitro. During culture, ovules were enlarged markedly, with opaque white color. When observed microscopically, there were cell divisions and cell clumps in embryo sacs. Subsequently, the cell clumps in embryo sacs ceased growth, likely because the integument grew faster than embryo sacs did and hindered the further development of embryo sacs. Therefore, subsequent callus morphogenesis might be originated from the integument. Thousands of calli from the two tomato varieties were obtained. Five diploid plants were regenerated after 15 months of subculturing. To eliminate the hindering effect of integument on embryo sac cells, the protoplasts of embryo sacs were prepared and cultured. After 48 hours of culture, the protoplasts of embryo sacs doubled in size and gradually formed clusters of cells. These results suggested that gynogenesis might be a potential way for haploid induction in tomato.
Abstract In order to determine the potential for haploid induction via in vitro gynogenesis in tomato, the ovules and protoplasts of embryo sacs from the hybrids Zhongza 101 and Zhongza 105 were cultured. An efficient method of ovule isolation was established in this study. Using this method, 100-150 ovules could be isolated from one ovary. Isolated ovules were cultured on three induction media to induce gynogenesis in vitro. During culture, ovules were enlarged markedly, with opaque white color. When observed microscopically, there were cell divisions and cell clumps in embryo sacs. Subsequently, the cell clumps in embryo sacs ceased growth, likely because the integument grew faster than embryo sacs did and hindered the further development of embryo sacs. Therefore, subsequent callus morphogenesis might be originated from the integument. Thousands of calli from the two tomato varieties were obtained. Five diploid plants were regenerated after 15 months of subculturing. To eliminate the hindering effect of integument on embryo sac cells, the protoplasts of embryo sacs were prepared and cultured. After 48 hours of culture, the protoplasts of embryo sacs doubled in size and gradually formed clusters of cells. These results suggested that gynogenesis might be a potential way for haploid induction in tomato.
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Received: 30 July 2013
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31171963) and the Major Project of Chinese National Programs for Fundamental Research and Development (2011CB100600). |
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Corresponding Authors:
WANG Xiao-xuan, Tel: +86-10-82109538, Fax: +86-10-62174123, E-mail: wangxiaoxuan@caas.cn
E-mail: wangxiaoxuan@caas.cn
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| About author: ZHAO He, E-mail: megahertz@sina.cn |
Cite this article:
ZHAO He, WANG Xiao-xuan, DU Yong-chen, ZHU De-wei, GUO Yan-mei, GAO Jian-chang, LI Fei , John C Snyder.
2014.
Haploid Induction via In vitro Gynogenesis in Tomato (Solanum lycopersicum L.). Journal of Integrative Agriculture, 13(10): 2122-2131.
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