|
|
|
|
|
| YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong |
| YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong |
| College of Horticulture, Shenyang Agricultural University/Key Laboratory for Northern Fruit Trees Cultivation and Physio-Ecology of Shenyang City, Shenyang 110866, P.R.China |
|
|
|
摘要 The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling (TUNEL) system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.
Abstract The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling (TUNEL) system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.
|
|
Received: 31 October 2012
Accepted:
|
| Fund: This study was supported by the Key Scientific and Technological Research Project of Shenyang City, China (1071154-3-00, F12-109-3-00) and the China Agriculture Research System (CARS-28). |
|
Corresponding Authors:
LIU Guo-cheng, Tel: +86-24-88487219; E-mail: liuguocheng2000@sina.com
E-mail: liuguocheng2000@sina.com
|
| About author: YANG Xu-yuan, E-mail: yangxuyuan320@sina.com; MA Huai-yu, Tel: +86-24-88487219, E-mail: shynydxgshzp@163.com |
Cite this article:
YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, Lü De-guo, QIN Si-jun , DU Guo-dong.
2014.
YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong. Journal of Integrative Agriculture, 13(4): 770-777.
|
| Ameisen J C. 2002. On the origin, evolution, and nature of programmed cell death: a timeline of four billion years. Cell Death and Differentiation, 9, 367-393 An L H, You R L. 2004. Studies on nuclear degeneration during programmed cell death of synergid and antipodal cells in Triticum aestivum. Journal of Plant Research, 17, 195-201 Cao J, Jiang F, Sodmergen, Cui K M. 2003. Time-course of programmed cell death during leaf senescence in Eucommia ulmoides. Journal of Plant Research, 116, 7-12 Chevalier P, Lingle S E. 1983. Sugar metabolism in developing kernels of wheat and barley. Crop Science, 23, 272-277 Coimbra S, Salema R. 1999. Ultrastructure of the developing and fertilized embryo sac of Amaranthus hypochondriacus L. Annals of Botany, 84, 781-789 Gaffal K P, Friedrichs G J, El-Gammal S. 2007. Ultrastructural evidence for a dual function of the phloem and programmed cell death in the floral nectary of Digitalis purpurea. Annals of Botany (London), 99, 593-607 Hauser B A, Sun K, Oppenheimer D G, Sage T L. 2006. Changes in mitochondrial membrane potential and accumulation of reactive oxygen species precede ultrastructural changes during ovule abortion. Planta, 223, 492-499 Liang C L, Liu M J, Zhao J. 2005. Research progress on plant seeds abortion. Molecular Plant Breeding, 3, 117- 122. (in Chinese) Li P H, Dong X Y, Wang Y Z, Yan S P, Liu C L. 2002. Study on the starch metabolism and ultrastructure of starch grain in apple fruit development process. Journal of Fruit Science, 19, 141-144 (in Chinese) Lu Y, Wang L, Pan H, Chen P, Wang D, Xie Y, Jin X X. 2011. Research on starch and protein accumulation and metabolism during the development of the ginkgo biloba female gametophyte. Acta Horticulturae Sinica, 38, 15- 24. (in Chinese) Li Z, Hu H L, Cao Y Q, Ren J, Leng P. 2008. Histological research on low seeded rate of hybridization between ‘mopansh’ and sweet persimmon. Acta Horticulturae Sinica, 35, 637- 642. (in Chinese) Mea M D, Serafini-Fracassini D, Duca S D. 2007. Programmed cell death: similarities and differences in animals and plants. A flower paradigm. Amino Acids, 33, 395-404 Otsuki Y, Li Z L, Shibata M A. 2003. Apoptotic detection methods: from morphology to gene.Progress in Histochemistry and Cytochemistry, 38, 275-339 Rie H, Osamu T. 2010. Pollen tube reuses intracellular components of nucellar cells undergoing programmed cell death in Pinus densiflora. Protoplasma, 248, 339- 351. Shu H R. 1999. Apple Science. Agriculture Press of China, China. p. 310. (in Chinese) Wan T, Xiao D X, Chang Q S. 2011. Studies on the embryogenesis and hybrid seeds abortion of Carica papaya. Journal of Fruit Science, 28, 699-702 (in Chinese) Yao J L, Fu C H, Hu C G. 2000. Abnormal phenomena during embryo development in Prunus salicina. Huazhong Agriculture University Transactions, 19, 71- 73. (in Chinese) Yang J P. 2006. Improvement of conventional paraffin section. Journal of Biology, 23, 45-46 (in Chinese) Zhao D Y, Liu G C, Lv D G, Qin S J, Ma H Y, Wang H. 2009. Evaluation of the specific characters of Hanfu apple cultivar. Journal of Fruit Science, 26, 6-12 (in Chinese) Zhang J, Lü L X, Ye M Z. 2006. Microscopic and submicroscopic observations on the abnormal embryonic development of embryo-abortive litchi (Litchi chinensis Sonn.cv. luhebao). Journal of Henan Agricultural University, 40, 194-197 (in Chinese) Zhou Z Q, Wang L K, Li W, Song X F, Yang C N. 2009. Study on programmed cell death and dynamic changes of starch accumulation in pericarp cells of Triticum aestivum L. Protoplasma, 236, 49-58. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
