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| Cell Production and Expansion in the Primary Root of Maize in Response to Low-Nitrogen Stress |
| GAO Kun, CHEN Fan-jun, YUAN Li-xing , MI Guo-hua |
| Key Laboratory of Plant-Soil Interaction, Ministry of Agriculture/Center for Resources, Environment and Food Security, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R.China |
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摘要 Maize plants respond to low-nitrogen stress by enhancing root elongation. The underlying physiological mechanism remains unknown. Seedlings of maize (Zea mays L., cv. Zhengdan 958) were grown in hydroponics with the control (4 mmol L-1) or low-nitrogen (40 μmol L-1) for 12 d, supplied as nitrate. Low nitrogen enhanced root elongation rate by 4.1-fold, accompanied by increases in cell production rate by 2.2-fold, maximal elemental elongation rate (by 2.5-fold), the length of elongation zone (by 1.5-fold), and final cell length by 1.8-fold. On low nitrogen, the higher cell production rate resulted from a higher cell division rate and in fact the number of dividing cells was reduced. Consequently, the residence time of a cell in the division zone tended to be shorter under low nitrogen. In addition, low nitrogen increased root diameter, an increase that occurred specifically in the cortex and was accompanied by an increase in cell number. It is concluded that roots elongates in response to low-nitrogen stress by accelerating cell production and expansion.
Abstract Maize plants respond to low-nitrogen stress by enhancing root elongation. The underlying physiological mechanism remains unknown. Seedlings of maize (Zea mays L., cv. Zhengdan 958) were grown in hydroponics with the control (4 mmol L-1) or low-nitrogen (40 μmol L-1) for 12 d, supplied as nitrate. Low nitrogen enhanced root elongation rate by 4.1-fold, accompanied by increases in cell production rate by 2.2-fold, maximal elemental elongation rate (by 2.5-fold), the length of elongation zone (by 1.5-fold), and final cell length by 1.8-fold. On low nitrogen, the higher cell production rate resulted from a higher cell division rate and in fact the number of dividing cells was reduced. Consequently, the residence time of a cell in the division zone tended to be shorter under low nitrogen. In addition, low nitrogen increased root diameter, an increase that occurred specifically in the cortex and was accompanied by an increase in cell number. It is concluded that roots elongates in response to low-nitrogen stress by accelerating cell production and expansion.
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Received: 08 April 2013
Accepted:
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| Fund: This work was financially supported by the National Natural Science Foundation of China (31071852 and 31121062). We thank Professor Tobias I Baskin (University of Massachusetts Amherst, USA) for the critical comments on the kinematic analysis and great assistance in manuscript preparation. |
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Corresponding Authors:
MI Guo-hua, E-mail: miguohua@cau.edu.cn
E-mail: miguohua@cau.edu.cn
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| About author: GAO Kun, Mobile: 15810536392, E-mail: happycreek@126.com |
Cite this article:
GAO Kun, CHEN Fan-jun, YUAN Li-xing , MI Guo-hua.
2014.
Cell Production and Expansion in the Primary Root of Maize in Response to Low-Nitrogen Stress. Journal of Integrative Agriculture, 13(11): 2508-2517.
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| Baskin T I, Cork A, Williamson R E, Gorst J R. 1995.STUNTED PLANT 1, a gene required for expansion inrapidly elongating but not in dividing cells and mediatingroot growth responses to applied cytokinin. PlantPhysiology, 107, 233-243Baskin T I, Remillong E L, Wilson J E. 2001. The impactof mannose and other carbon sources on the elongationand diameter of the primary root of Arabidopsis thaliana.Australian Journal of Plant Physiology, 8, 481-488Baskin T I. 2013. Patterns of root growth acclimation: Constantprocesses, changing boundaries. WIREs DevelopmentalBiology, 2, 65-73Beemster G T S, Baskin T I. 1998. Analysis of cell divisionand elongation underlying the developmental accelerationof root growth in Arabidopsis thaliana. Plant Physiology,116, 1515-1526Beemster G T S, Baskin T I. 2000. STUNTED PLANT 1mediates effects of cytokinin, but not of auxin, on celldivision and expansion in the root of Arabidopsis. PlantPhysiology, 124, 1718-1727Beman J M, Arrigo K R, Matson P A. 2005. Agricultural runofffuels large phytoplankton blooms in vulnerable areas ofthe ocean. Nature, 434, 211-214Bengough A G, McKenzie B, Hallett P, Valentine T. 2011.Root elongation, water stress, and mechanical impedance:A review of limiting stresses and beneficial root tip traits.Journal of Experimental Botany, 62, 59-68Bosemark N O. 1954. The influence of nitrogen on rootdevelopment. Physiologia Plantarum, 7, 497-502Clark L J, Price A H, Steele K A, Whalley W R. 2008. Evidencefrom near-isogenic lines that root penetration increaseswith root diameter and bending stiffness in rice. FunctionalPlant Biology, 35, 1163-1171De Cnodder T, Verbelen J P, Vissenberg K. 2007. The controlof cell size and rate of elongation in the Arabidopsisroot. In: Plant Cell Monographs. 5th ed. Springer, BerlinHerdelberg. pp. 249-269Darzynkiewicz Z, Andreef M, Tranganos F, Sharpless T,Melamed M R. 1978. Discrimination of cycling and noncyclinglymphocytes by BUdR-suppressed acridine orangefluorescence in a flow cytometric system. ExperimentalCell Research, 115, 31-35Dewitte W, Riou-Khamlichi C, Scofield S, Healy J M S,Jacqmard A, Kilby N J, Murray J A H. 2003. Altered cellcycle distribution, hyperplasia, and inhibited differentiationin Arabidopsis caused by the D-type cyclin CYCD3. ThePlant Cell Online, 15, 79-92Downes B P, Steinbaker C R, Crowell D N. 2001. Expressionand processing of a hormonally regulated β-expansin fromsoybean. Plant Physiology, 126, 244-252Flynn K, Davidson K, Leftley J. 1994. Carbon-nitrogenat whole-cell and free-amino-acid levels during batchgrowth of Isochrysis galbana (Prymnesiophyceae) underconditions of alternating light and dark. Marine Biology,118, 229-237Gastal F, Nelson C J. 1994. Nitrogen use within the growingleaf blade of tall fescue. Plant Physiology, 105, 191-197Gaudin A C M, McClymont S A, Holmes B M, Lyons E,Raizada M N. 2011. Novel temporal, fine-scale and growthvariation phenotypes in roots of adult-stage maize (Zeamays L.) in response to low nitrogen stress. Plant, Celland Environment, 34, 2122-2137Greef J M, Geisler G. 1988. Growth of isolated maize root tipsat various levels of N supply. Mitteilungen der Gesellschaftfur Pflanzenbauwissenschaften, 1, 63-65Hirel B, Le Gouis J, Ney B, Gallais A. 2007. The challengeof improving nitrogen use efficiency in crop plants:Towards a more central role for genetic variability andquantitative genetics within integrated approaches. Journalof Experimental Botany, 58, 2369-2387Itoh S, Barber S A. 1983. Phosphorus uptake by six plantspecies as related to root hairs. Agronomy Journal, 75,457-461Ivanov V B, Dubrovsky J G. 1997. Estimation of the cellcycleduration in the root apical meristem: A model oflinkage between cell-cycle duration, rate of cell production,and rate of root growth. International Journal of PlantSciences, 158, 757-763Kavanová M, Lattanzi F A, Grimoldi A A, Schnyder H.2006. Phosphorus deficiency decreases cell division andelongation in grass leaves. Plant Physiology, 141, 766-775Kavanová M, Lattanzi F A, Schnyder H. 2008. Nitrogendeficiency inhibits leaf blade growth in Lolium perenne byincreasing cell cycle duration and decreasing mitotic andpost-mitotic growth rates. Plant, Cell and Environment,31, 727-737Kiba T, Kudo T, Kojima M, Sakakibara H. 2011. Hormonalcontrol of nitrogen acquisition: roles of auxin, abscisicacid, and cytokinin. Journal of Experimental Botany, 62,1399-1409Kubica Š, Baluška F. 1988. Maize primary root growth anddifferentiation under conditions of nitrate over-supply.Biológia (Bratislava), 44, 407-414Li Q, Li B H, Kronzucker H J, Shi W M. 2010. Root growthinhibition by NH4+ in Arabidopsis is mediated by the roottip and is linked to NH4+ efflux and GMPase activity. Plant, Cell and Environment, 33, 1529-1542London J G. 2005. Nitrogen study fertilizes fears of pollution.Nature, 433, 791-791Lynch J P. 2007. Roots of the second green revolution.Australian Journal of Botany, 55, 493-512Lynch J. 1995. Root architecture and plant productivity. PlantPhysiology, 109, 7-13Morris A K, Silk W K. 1992. Use of a flexible logistic functionto describe axial growth of plants. Bulletin of MathematicalBiology, 54, 1069-1081Del Pozo J C, Lopez-Matas M, Ramirez-Parra E, Gutierrez C.2004. Hormonal control of the plant cell cycle. PhysiologiaPlantarum, 123, 173-183Rahayu Y S, Walch-Liu P, Neumann G, Römheld V, VonWirén N, Bangerth F. 2005. Root-derived cytokinins aslong-distance signals for NO3--induced stimulation of leafgrowth. Journal of Experimental Botany, 56, 1143-1152Roggatz U, McDonald A, Stadenberg I, Schurr U. 1999.Effects of nitrogen deprivation on cell division andexpansion in leaves of Ricinus communis L. Plant, Celland Environment, 22, 81-89Rounds C M, Lubeck E, Hepler P K, Winship L J. 2011.Propidium Iodide competes with Ca2+ to label pectin inpollen tubes and Arabidopsis root hairs. Plant Physiology,157, 175-187Sharp R E, Silk W K, Hsiao T C. 1988. Growth of the maizeprimary root at low water potentials. 1. Spatial distributionof expansive growth. Plant Physiology, 87, 50-57Silk W K, Lord E M, Eckard K J. 1989. Growth patternsinferred from anatomical records empirical tests usinglongisections of roots of Zea mays L. Plant Physiology,90, 708-713Silk W K. 1992. Steady form from changing cells. InternationalJournal of Plant Sciences, 153, 49-58Thaler P, Pages L. 1996. Root apical diameter and rootelongation rate of rubber seedlings (Hevea brasiliensis)show parallel responses to photoassimilate availability.Physiologia Plantarum, 97, 365-371Tian Q Y, Chen F J, Liu J X, Zhang F S, Mi G H. 2008.Inhibition of maize root growth by high nitrate supply iscorrelated with reduced IAA levels in roots. Journal ofPlant Physiology, 165, 942-951Tian Q Y, Chen F J, Zhang F S, Mi G H. 2005. Possibleinvolvement of cytokinin in nitrate-mediated root growthin maize. Plant and Soil, 277, 185-196De Veylder L, Beeckman T, Beemster G T S, Krols L, TerrasF, Landrieu I, Van Der Schueren E, Maes S, Naudts M,Inzé D. 2001. Functional analysis of cyclin-dependentkinase inhibitors of Arabidopsis. The Plant Cell Online,13, 1653-1668Wang Y, Mi G H, Chen F J, Zhang J H, Zhang F S. 2004.Response of root morphology to nitrate supply and itscontribution to nitrogen accumulation in maize. Journalof Plant Nutrition, 27, 2189-2202Yamaguchi M, Valliyodan B, Zhang J, Lenobie M E, Yu O,Rogers E E, Nguyen H T, Sharp R E. 2010. Regulation ofgrowth response to water stress in the soybean primary root.I. Proteomic analysis reveals region-specific regulation ofphenylpropanoid metabolism and control of free iron in theelongation zone. Plant, Cell and Environment, 33, 223-243 |
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