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| Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae |
| LI Xue-song, XU Fei, WANG Hong-kai , LIN Fu-cheng |
1.State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, P.R.China
2.Bioinformatics Lab, Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China |
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摘要 The rice blast, caused by fungus Magnaporthe oryzae, is a major constraint to the world food security. Hyphal growth is the foundation of fungal development and proliferation of fungi. To investigate genes involved in hyphal growth of this fungus, digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity, with its related wildtype strain. 416 genes were detected as differential expression, 178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant. Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuolerelated processes. The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M. oryzae pathogenesis.
Abstract The rice blast, caused by fungus Magnaporthe oryzae, is a major constraint to the world food security. Hyphal growth is the foundation of fungal development and proliferation of fungi. To investigate genes involved in hyphal growth of this fungus, digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity, with its related wildtype strain. 416 genes were detected as differential expression, 178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant. Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuolerelated processes. The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M. oryzae pathogenesis.
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Received: 04 January 2013
Accepted:
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| Fund: This study was supported by the Natural Science Foundation of Zhejiang Province, China (Y3110028 and LQ12C14003). |
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Corresponding Authors:
WANG Hong-kai, E-mail: hkwang@zju.edu.cn
E-mail: hkwang@zju.edu.cn
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Cite this article:
LI Xue-song, XU Fei, WANG Hong-kai , LIN Fu-cheng.
2013.
Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae. Journal of Integrative Agriculture, 12(12): 2189-2196.
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| [1]Akmaev V R, Wang C J. 2004. Correction of sequence- based artifacts in serial analysis of gene expression. Bioinformatics, 20, 1254-1263[2]Altschul S F, Madden T L, Schäffer A A, Zhang J, Zhang Z, Miller W, Lipman D J. 1997. Gapped BLAST and PSI- BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402[3]Anisimov S V. 2008. Serial analysis of gene expression (SAGE): 13 years of application in research. Current Pharmaceutical Biotechnology, 9, 338-350[4]Barrett T, Troup D B, Wilhite S E, Ledoux P, Rudnev D, Evangelista C, Kim I F, Soboleva A, Tomashevsky M, Marshall K A, et al. 2009. NCBI GEO: archive for high-throughput functional genomic data. Nucleic Acids Research, 37, D885-D890. [5]Bhadauria V, Popescu L, Zhao W S, Peng Y L. 2007. Fungal transcriptomics. Microbiological Research, 162, 285- 298. [6]Boyle E I, Weng S, Gollub J, Jin H, Botstein D, Cherry J M, Sherlock G. 2004. GO::TermFinder - open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes. Bioinformatics, 20, 3710- 3715.[7]Caracuel-Rios Z, Talbot N J. 2007. Cellular differentiation and host invasion by the rice blast fungus Magnaporthe grisea. Current Opinion in Microbiology, 10, 339-345[8]Casselton L, Zolan M. 2002. The art and design of genetic screens: filamentous fungi. Nature Reviews Genetics, 3, 683-697[9]Dean R A, Talbot N J, Ebbole D J, Farman M L, Mitchell T K, Orbach M J, Thon M, Kulkarni R, Xu J R, Pan H, et al. 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 434, 980-986[10]Gowda M, Venu R C, Raghupathy M B, Nobuta K, Li H, Wing R, Stahlberg E, Couglan S, Haudenschild C D, Dean R, et al. 2006. Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods. BMC Genomics, 7, 310. [11]Harris S D, Cheng J, Pugh T A, Pringle J R. 1992. Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations. Journal of Molecular Biology, 225, 53-65[12]Hoeberichts F A, Vaeck E, Kiddle G, Coppens E, van de Cotte B, Adamantidis A, Ormenese S, Foyer C H, Zabeau M, Inzé D, et al. 2008. A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. Journal of Molecular Biology, 283, 5708-5718[13]Hong L Z, Li J, Schmidt-Küntzel A, Warren W C, Barsh G S. 2011. Digital gene expression for non-model organisms. Genome Research, 21, 1905-1915[14]Irie T, Matsumura H, Terauchi R, Saitoh H. 2003. Serial analysis of gene expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation. Molecular Genetics and Genomics, 270, 181-189[15]Jeon J, Choi J, Park J, Lee Y H. 2008. Functional genomics in the rice blast fungus to unravel the fungal pathogenicity. Journal of Zhejiang University Science (B), 9, 747-752[16]Jeon J, Park S Y, Chi M H, Choi J, Park J, Rho H S, Kim S, Goh J, Yoo S, Choi J, et al. 2007. Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nature Genetics, 39, 561-565[17]Kabsch W, Sander C. 1984. On the use of sequence homologies to predict protein structure: identical pentapeptides can have completely different conformations. Proceedings of the National Academy of Sciences of the United States of America, 81, 1075-1078[18]Kim S G, Kim S T, Kim S K, Kang K Y. 2008. Gene expression profiling in rice infected with rice blast fungus using SAGE. Plant Pathology Journal, 24, 384- 391.[19]Kong L A, Yang J, Li G T, Qi L L, Zhang Y J, Wang C F, Zhao W S, Xu J R, Peng Y L. 2012. Different chitin synthase genes are required for various developmental and plant infection processes in the rice blast fungus Magnaporthe oryzae. PLoS Pathogens, 8, e1002526. [20]Lash A E, Tolstoshev C M, Wagner L, Schuler G D, Strausberg R L, Riggins G J, Altschul S F. 2000. SAGEmap: a public gene expression resource. Genome Research, 10, 1051-1060[21]Mathioni S M, Beló A, Rizzo C J, Dean R A, Donofrio N M 2011. Transcriptome profiling of the rice blast fungus during invasive plant infection and in vitro stresses. BMC Genomics, 12, 49.[22]Matsumura H, Bin Nasir K H, Yoshida K, Ito A, Kahl G, Krüger D H, Terauchi R. 2006. SuperSAGE array: the direct use of 26-base-pair transcript tags in oligonucleotide arrays. Nature Methods, 3, 469-474[23]Matsumura H, Reich S, Ito A, Saitoh H, Kamoun S, Winter P, Kahl G, Reuter M, Kruger D H, Terauchi R. 2003. Gene expression analysis of plant host-pathogen interactions by SuperSAGE. Proceedings of the National Academy of Sciences of the United States of America, 100, 15718-15723[24]Mondal K, Dastidar A G, Singh G, Madhusudhanan S, Gande S L, VijayRaghavan K, Varadarajan R. 2007. Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila. Journal of Molecular Biology, 370, 939-950[25]Mortazavi A, Williams B A, McCue K, Schaeffer L, Wold B. 2008. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nature Methods, 5, 621- 628. Mullins E D, Kang S. 2001. Transformation: a tool for studying fungal pathogens of plants. Cellular and Molecular Life Sciences, 58, 2043-2052[26]Rost B. 1999. Twilight zone of protein sequence alignments. Protein Engineering, 12, 85-94[27]Soundararajan S, Jedd G, Li X, Ramos-Pamploña M, Chua N H, Naqvi N I. 2004. Woronin body function in Magnaporthe grisea is essential for efficient pathogenesis and for survival during nitrogen starvation stress. The Plant Cell, 16, 1564-1574[28]Hoen P A, Ariyurek Y, Thygesen H H, Vreugdenhil E, Vossen R H, de Menezes R X, Boer J M, van Ommen G J, den Dunnen J T. 2008. Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray platforms. Nucleic Acids Research, 36, e141. [29]Talbot N J. 2003. On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea. Annual Review of Microbiology, 57, 177-202[30]Teyssier E, Hirokawa G, Tretiakova A, Jameson B, Kaji A, Kaji H. 2003. Temperature-sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF). Nucleic Acids Research, 31, 4218-4226[31]Tucker S L, Talbot N J. 2001. Surface attachment and pre- penetration stage development by plant pathogenic fungi. Annual Review of Microbiology, 39, 385-417[32]Viaud M C, Balhadère P V, Talbot N J. 2002. A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection. The Plant Cell, 14, 917-930[33]Wang H K, Lin F C, Li D B. 2007. Genetic analysis on temperature sensitive mutants in Magnaporthe grisea. International Conference on Integration of Science & Technology for Sustainable Development, 1, 152-155[34]Wang Y, Jiao T, Liu X, Lin F, Wu W. 2011 Functional Characterization of a NEM1-like gene in Magnaporthe oryzae. Journal of Integrative Agriculture, 10, 1385- 1390. [35]Xu F, Liu X H, Zhuang F L, Zhu J, Lin F C. 2011. Analyzing autophagy in Magnaporthe oryzae. Autophagy, 7, 525-530[36]Yang J, Kong L, Chen X, Wang D, Qi L L, Zhao W, Zhang Y, Liu X, Peng Y L. 2012. A carnitine-acylcarnitine carrier protein, MoCrc1, is essential for pathogenicity in Magnaporthe oryzae. Current Genetics, 58, 139-148. |
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