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| Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings |
| DONG Yu-xiu, WANG Xiu-feng , CUI Xiu-min |
1 Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2 College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, P.R.China |
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摘要 Nitric oxide (NO), a bioactive signaling molecule, serves as an antioxidant and anti-stress agent under abiotic stress. A hydroponics experiment was conducted to investigate the effects of sodium nitroprusside (SNP), a NO donor, on tomato seedlings exposed to 50 μmol L-1 CuCl2. The results show that copper is primarily stored in the soluble cell sap fraction in the roots, especially after treatment with Cu+SNP treatment, which accounted for 66.2% of the total copper content. The copper concentration gradually decreased from the roots to the leaves. In the leaves, exogenous NO induces the storage of excess copper in the cell walls. Copper stress decreases the proportion of copper integrated with pectates and proteins, but exogenous NO remarkably reverses this trend. The alleviating effect of NO is blocked by hemoglobin. Thus, exogenous NO is likely involved in the regulation of the subcellular copper concentrations and its chemical forms under copper stress. Although exogenous NO inhibited the absorption and transport of excess copper to some extent, the copper accumulation in tomato seedlings significantly increased under copper stress. The use of exogenous NO to enhance copper tolerance in some plants is a promising method for copper remediation.
Abstract Nitric oxide (NO), a bioactive signaling molecule, serves as an antioxidant and anti-stress agent under abiotic stress. A hydroponics experiment was conducted to investigate the effects of sodium nitroprusside (SNP), a NO donor, on tomato seedlings exposed to 50 μmol L-1 CuCl2. The results show that copper is primarily stored in the soluble cell sap fraction in the roots, especially after treatment with Cu+SNP treatment, which accounted for 66.2% of the total copper content. The copper concentration gradually decreased from the roots to the leaves. In the leaves, exogenous NO induces the storage of excess copper in the cell walls. Copper stress decreases the proportion of copper integrated with pectates and proteins, but exogenous NO remarkably reverses this trend. The alleviating effect of NO is blocked by hemoglobin. Thus, exogenous NO is likely involved in the regulation of the subcellular copper concentrations and its chemical forms under copper stress. Although exogenous NO inhibited the absorption and transport of excess copper to some extent, the copper accumulation in tomato seedlings significantly increased under copper stress. The use of exogenous NO to enhance copper tolerance in some plants is a promising method for copper remediation.
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Received: 20 September 2012
Accepted:
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| Fund: This work was supported partially by the National Natural Science Foundation of China (31201619), Profession Expert Group of Facility Cultivation and Engineering (CARS- 25-D-03) and the Sci-Tech Development Project of Tai’an City, China (32606). |
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Corresponding Authors:
Correspondence CUI Xiu-min, Tel: +86-538-8241546, Mobile: 13905489073, Fax: +86-538-8242250, E-mail: xiumincui@sdau.edu.cn
E-mail: xiumincui@sdau.edu.cn
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| About author: DONG Yu-xiu, E-mail: zwfy1@sdau.edu.cn; |
Cite this article:
DONG Yu-xiu, WANG Xiu-feng , CUI Xiu-min.
2013.
Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings. Journal of Integrative Agriculture, 12(10): 1783-1790.
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