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Journal of Integrative Agriculture  2023, Vol. 22 Issue (7): 1951-1966    DOI: 10.1016/j.jia.2023.02.031
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Advancing approach and toolbox in optimization of chloroplast genetic transformation technology
LIU Yu-xin1, 2, LI Fan1, 2, GAO Liang1, 3, TU Zhang-li1, 2, ZHOU Fei1, 2#, LIN Yong-jun1, 2

1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China

2 Hubei Hongshan Laboratory, Wuhan 430070, P.R.China.

3 Wuhan Towin Biotechnology Co., Ltd., Wuhan 430070, P.R.China

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Chloroplast is a discrete, highly structured, and semi-autonomous cellular organelle. The small genome of chloroplast makes it an up-and-coming platform for synthetic biology. As a special means of synthetic biology, chloroplast genetic engineering shows excellent potential in reconstructing various sophisticated metabolic pathways within the plants for specific purposes, such as improving crop photosynthetic capacity, enhancing plant stress resistance, and synthesizing new drugs and vaccines. However, many plant species exhibit limited efficiency or inability in chloroplast genetic transformation. Hence, new transformation technologies and tools are being constantly developed. In order to further expand and facilitate the application of chloroplast genetic engineering, this review summarizes the new technologies in chloroplast genetic transformation in recent years and discusses the choice of appropriate synthetic biological elements  for the construction of efficient chloroplast transformation vectors.

Keywords:  chloroplast        genetic engineering        new technology        plasmid optimization        nanotechnology  
Received: 18 November 2022   Accepted: 12 January 2023
Fund: This work was funded by the Foundation of Hubei Hongshan Laboratory, China (2022hszd014) and the National Natural Science Foundation of China (31771752).
About author:  #Correspondence ZHOU Fei, Tel: +86-27-87280550,

Cite this article: 

LIU Yu-xin, LI Fan, GAO Liang, TU Zhang-li, ZHOU Fei, LIN Yong-jun. 2023. Advancing approach and toolbox in optimization of chloroplast genetic transformation technology. Journal of Integrative Agriculture, 22(7): 1951-1966.

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