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Journal of Integrative Agriculture  2013, Vol. 12 Issue (12): 2157-2163    DOI: 10.1016/S2095-3119(13)60345-7
Physiology & Biochentry · Tillage · Cultivation Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions
 ZHANG Ru-biao, HU Hai-juan, ZHAO Zheng, YANG Dan-dan, ZHU Xin-kai, GUO Wen-shan
1 Key Lab of Crop Genetics and Physiology, Jiangsu Province/Wheat Research Institute, Yangzhou University, Yangzhou 225009, P.R.China
2 State Key Laboratory of Soil and Sustainable Agriculture, Ministry of Science and Technology/Institute of Soil Sciences, Chinese Academy of Sciences, Nanjing 210008, P.R.China
3 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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摘要  O3 is not only greenhouse gas but also a primary gaseous contaminant in the atmosphere. It has long-lasting effects on crop growth, yield and quality, and brings a series of ecological and environmental problems. A free-air controlled enrichment (FACE) system was applied to study the effect of elevated ozone concentration on activities of key enzymes of starch synthesis of Yangmai 16 in 2009-2010. The main-plot treatment had two levels of O3: ambient level (A-O3) and 50% higher than ambient level (E-O3). The main results were that accumulation rate of amylose, amylopectin and starch were represented in a single peak curve, and their content and accumulation amount rose gradually. The O3 elevation decreased the accumulation rate of amylose, amylopectin and starch amylase, reduced the accumulation amount of amylopectin and starch, and decreased the content of amylopectin and starch, but increased the content of amylose. With the increase of O3 concentration, the enzyme activity of grain granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) decreased after anthesis. The activities of GBSS and SSS had highly significant correlations with amylose, amylopectin and starch accumulation rate, and the activity of SBE had significant correlations with these items. So the O3 elevation decreased the activity of key enzymes of starch synthesis, which led to the variation of starch synthesis.

Abstract  O3 is not only greenhouse gas but also a primary gaseous contaminant in the atmosphere. It has long-lasting effects on crop growth, yield and quality, and brings a series of ecological and environmental problems. A free-air controlled enrichment (FACE) system was applied to study the effect of elevated ozone concentration on activities of key enzymes of starch synthesis of Yangmai 16 in 2009-2010. The main-plot treatment had two levels of O3: ambient level (A-O3) and 50% higher than ambient level (E-O3). The main results were that accumulation rate of amylose, amylopectin and starch were represented in a single peak curve, and their content and accumulation amount rose gradually. The O3 elevation decreased the accumulation rate of amylose, amylopectin and starch amylase, reduced the accumulation amount of amylopectin and starch, and decreased the content of amylopectin and starch, but increased the content of amylose. With the increase of O3 concentration, the enzyme activity of grain granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) decreased after anthesis. The activities of GBSS and SSS had highly significant correlations with amylose, amylopectin and starch accumulation rate, and the activity of SBE had significant correlations with these items. So the O3 elevation decreased the activity of key enzymes of starch synthesis, which led to the variation of starch synthesis. Key words:
Keywords:  O3       FACE       wheat       starch       activity of enzymes  
Received: 29 October 2012   Accepted:
Fund: 

This work was supported by the Innovation Program of Chinese Academy of Sciences (KZCX2-EW-414), the International S&T Cooperation Program of China (2009DFA31110), the Qing Lan Project Knowledge, Priority Academic Program Development of Jiangsu Higher Education Institutions, China and the Global Environment Research Fund, Ministry of the Environment, Japan (C- 062). We are grateful to Prof. Liu Gang, Chinese Academy of Sciences, for his technical support in the free-air ozone release system.

Corresponding Authors:  ZHU Xin-kai, Tel/Fax: +86-514-87979300, E-mail: xkzhu@yzu.edu.cn; GUO Wen-shan, Tel: +86-514-87979339, E-mail: guows@yzu.edu.cn     E-mail:  xkzhu@yzu.edu.cn;guows@yzu.edu.cn

Cite this article: 

ZHANG Ru-biao, HU Hai-juan, ZHAO Zheng, YANG Dan-dan, ZHU Xin-kai, GUO Wen-shan. 2013. Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions. Journal of Integrative Agriculture, 12(12): 2157-2163.

[1]Bai Y M, Guo J P, Liu L, Wen M. 2001. Influences of ozone on the leaf injury photosynthesis and yield of rice. Meteorological Monthly, 27, 17-21 (in Chinese)

[2]Bai Y M, Guo J P, Wang C Y, Wen M. 2002. The reaction and sensitivity experiment of O3 on rice and winter wheat. Chinese Journal of Eco-Agriculture, 10, 13-16. (in Chinese)

[3]Cheng F M, Jiang D A, Wu P, Shi C H. 2001. The dynamic change of starch synthesis enzymes during the grain filling stage and effects of temperature upon it. Acta Agronomica Sinica, 27, 201-206. (in Chinese)

[4]Douglas C D, Tsung M K, Frederick C F. 1988. Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize. Plant Physiology, 86, 1013-1019

[5]Feng Z Z, Kobayashi K, Wang X K, Feng Z W. 2009. A meta-analysis of responses of wheat yield formation to elevated ozone concentration. Chinese Science Bulletin, 54, 249-255

[6]Feng Z Z, Pang J, Kobayashi K, Yamakawa T, Zhu J. 2011. Differential responses in two varieties of winter wheat to elevated ozone concentration under fully open-air field conditions. Global Change Biology, 17, 580-591

[7]Fowler D, Cape J N, Coyle M, Smith R, Hiellbrekke A G, Simpson D, Derwent R G, Johnson C E. 1999. Modelling photochemical oxidant formation, transport, deposition and exposure of terrestrial ecosystems. Environmental Pollution, 100, 43-55

[8]Guderian R. 1985. Emissions and ambient ozone concentration. In: Guderian R, ed., Air Pollution by Photochemical Oxidants: Formation, Transport, Control and Effects on Plant. Springer, Berlin. pp. 11-67

[9]Guo J P, Wang C Y, Bai Y M, Wen M, Huo Z G, Liu J G, Li L. 2001. Effects of elevated ozone concentration on the physiological processes and grain quality of winter wheat. Journal of Applied Meteorology, 12, 255-256 (in Chinese)

[10]He Z F. 1985. Quality of Cereals, Oils and Analysis Techniques. Beijing Agricultural Press, China. pp. 290- 301. (in Chinese)

[11]Hertstein U, Grtinhage L, Jager H J. 1995. Assessment of past, present and future impacts of ozone and carbon dioxide on crop yields. Atmosphere Environment, 29, 2031-2039

[12]Hu Y Y, Zhao T H, Xu L, Sun J W, Fu Y, Ma J, Zhou M. 2008. Effects of elevated O3 exposure on photosynthesis and yield in wheat. Research of Agricultural Modernization, 7, 498-502 (in Chinese)

[13]Karnosky D F, Skelly J M, Percy K E, Chappelka A H. 2007a. Perspectives regarding 50 years of research on effects of tropospheric ozone air pollution on US forests. Environmental Pollution, 147, 489-506

[14]Karnosky D F, Werner H, Holopainen T, Percy K, Oksanen T, Oksanen E, Heerdt C, Fabian P, Nagy J, Heilman W, Cox R, Nelson N, Matyssek R. 2007b. Free-air exposure systems to scale up ozone research to mature trees. Plant Biology, 9, 181-190

[15]Keeling P L, Bacon P J, Holt D C. 1993. Elevated temperature reduce starch deposition in wheat endosperm by reducing the acitity of soluble starch synthase. Planta, 191, 342-348

[16]Li C Y, Feng C N, Zhang Y, Guo W S, Zhu X K, Peng Y X. 2005. Effects of the ratio between basal N and top dressing N on grain starch formation in weak gluten wheat variety Ningmai 9 and its enzymes activities. Scientia Agricultura Sinica, 38, 1120-1125

[17](in Chinese) Li T G, Shen B, Cheng N, Luo Y K. 1997. Effect of Q-enzyme on the chalkiness formation of rice grain. Acta Agronomica Sinica, 23, 338-344. (in Chinese)

[18]Liang J S, Cao X Z, Xu S, Zhu Q S, Song P. 1994. Studies on the relationship between the grain sink strength and it’s starch accumulation in rice (O. sativa). Acta Agronomica Sinica, 20, 685-691. (in Chinese)

[19]Liu X, Jiang C M, Zheng Z R, Zhou Z N, He M R, Wang Z L. 2005. Activities of the enzymes involved in starch synthesis and starch accumulation in grains of wheat cultivars GC8901 and SN1391. Scientia Agricultura Sinica, 38, 897-903 (in Chinese)

[20]Meehl G A, Stocker T F, Collins W D, Friedlingstein P, Gaye A T, Gregory J M, Kitoh A, Knutti R, Murphy J M, Noda A, et al. 2007. Global Climate Projections. Cambridge University Press, Cambridge.

[21]Morgan P B, Mies T A, Bollero G A, Nelson R L, Long S P. 2006. Season-long elevation of ozone concentration to projected 2050 levels under fully open-air conditions substantially decreases the growth and production of soybean. New Phytologist, 170, 333-343

[22]Nakamura Y, Kuki K, Park S Y, Ohya T. 1989. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiology, 30, 833-839

[23]Prather M, Gauss M, Berntsen T, Isaksen I, Sundet J, Bey I, Brasseur G, Dentener F, Derwent R, Stevenson D, et al. 2003. Fresh air in the 21st century. Geophysical Research Letters, 30, 1100.

[24]Rahman S, Abrahams S, Abbott D, Mukai Y, Samuel M, Morell M, Appels R. 1997. A complex arrangement of genes at a starch branching enzyme I locus in the D-genome donor of wheat. Genome, 40, 465-474

[25]Schaffer A A, Petreikov M. 1997. Sucrose-to-starch metabolism in tomato fruit undergoing transient starch accumulation. Plant Physiology, 113, 739-746

[26]Wang C Y, Guo J P, Bai Y M, Wen M, Liu J P, Liu J G, Li L. 2002. Experimental study of impacts by increasing ozone concentration on winter wheat. Meteorologica Sinica, 4, 238-242 (in Chinese)

[27]Wang L, Zeng Q, Feng Z Z, Zhu J G, Tang H Y, Chen X, Xie Z B, Liu G, Kobayashi K. 2009. Photosynthetic damage induce by elevated ozone in two varieties of wheat with free air controlled enrichment approach. Environmental Science, 30, 527-534

[28](in Chinese) Wang Y F, Yu Z W, Li S X, Yu S L. 2003. Activity of enzymes related to starch synthesis and their effect during the filling of winter wheat. Acta Agronomica Sinica, 29, 75-81

[29]Xie J Q, Zheng Q W, Wang X K, Zhang B J. 2006. Effect of ozone on photosynthesis of rice leaves, ear character and yield component in situe. Acta Agriculturae Boreail Occidentalis Sinica, 15, 27-30. (in Chinese)

[30]Zhang F, Jiang D A, Wong X Y. 2001. Recent advances on enzymology and molecular biology of starch synthase. Chinese Bulletin of Botany, 18, 177-182. (in Chinese)

[31]Zheng Y F, Liu H J, Wu R J, Zhao Z, Hu C D, Shi C H. 2010. Effects of elevated surface ozone stress on grain quality in winter wheat. Journal of Agro-Environment Science, 29, 3080-3085 (in Chinese)

[32]Zhu X K, Feng Z Z, Sun T F, Liu X C, Tang H Y, Zhu J G, Guo W S, Kobayashi K. 2011. Effects of elevated ozone concentration on yield of four Chinese cultivars of winter wheat under fully open-air field conditions. Global Change Biology, 17, 2697-2706

[33]Zhu X K, Liu X C, Sun T F, Guo W S, Zhu J G, Feng Z Z, Kobayashi K. 2011. Effects of elevated ozone concentration on grain yield and plumpness in wheat with FACE system. Scientia Agricultura Sinica, 44, 1100-1108. (in Chinese)

[34]Zhu X K, Liu X C, Sun T F, Guo W S, Zhu J G, Kobayashi K. 2010. Effects of elevated ozone concentration on wheat grain protein components with FACE system. Chinese Journal of Applied Ecology, 21, 2551-2557. (in Chinese)
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