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| Single and Combined Effects of As (III) and Acetochlor on Phosphatase Activity in Soil |
| ZHANG Yun, ZHANG Feng, ZHANG Guan-cai , GUAN Lian-zhu |
1 College of Land and Environment, Shenyang Agriculture University, Shenyang 110866, P.R.China
2 The Center for Agriculture Quantity and Safe, Ministry of Agriculture, Beijing 100081, P.R.China |
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摘要 The actions and interactions of acetochlor and As on the soil phosphatase activity were investigated after 1, 3, 6, 10, 15, 30 and 60 d of exposure under control conditions. The soils were exposed to various concentrations of acetochlor and As individually and simultaneously. The results showed that acetochlor, As only, and combined pollution all clearly inhibited soil phosphatase activity. The maximum inhibition ratios of soil phosphatase activity by acetochlor, As only and combined pollution were 36.44, 74.12 and 61.29%, respectively. Two kinetic models, ν=c/(1+bi) (model 1) and ν=c(1+ai)/(l+bi) (model 2), were used to describe the relationship between the concentrations of As and acetochlor and the activity of soil phosphatase. The semi-effect dose (ED50) values induced by As and acetochlor stress based on the inhibition of soil phosphatase were 18.1 and 33.11 mg kg-1, respectively, according to calculation by model 1. The interactive effect of acetochlor with As on soil phosphatase primarily consisted of significant antagonism effects at the higher concentrations tested. The step regression results show that the toxicity order was As (III)>acetochlor>As (III)×acetochlor throughout the incubation period.
Abstract The actions and interactions of acetochlor and As on the soil phosphatase activity were investigated after 1, 3, 6, 10, 15, 30 and 60 d of exposure under control conditions. The soils were exposed to various concentrations of acetochlor and As individually and simultaneously. The results showed that acetochlor, As only, and combined pollution all clearly inhibited soil phosphatase activity. The maximum inhibition ratios of soil phosphatase activity by acetochlor, As only and combined pollution were 36.44, 74.12 and 61.29%, respectively. Two kinetic models, ν=c/(1+bi) (model 1) and ν=c(1+ai)/(l+bi) (model 2), were used to describe the relationship between the concentrations of As and acetochlor and the activity of soil phosphatase. The semi-effect dose (ED50) values induced by As and acetochlor stress based on the inhibition of soil phosphatase were 18.1 and 33.11 mg kg-1, respectively, according to calculation by model 1. The interactive effect of acetochlor with As on soil phosphatase primarily consisted of significant antagonism effects at the higher concentrations tested. The step regression results show that the toxicity order was As (III)>acetochlor>As (III)×acetochlor throughout the incubation period.
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Received: 30 October 2012
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (41101455). |
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Corresponding Authors:
Correspondence GUAN Lian-zhu, Tel: +86-24-88493104, E-mail: guanlianzhu1960@163.com
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| About author: ZHANG Yun, Tel: +86-24-88493104, E-mail: xingyun92757@163.com |
Cite this article:
ZHANG Yun, ZHANG Feng, ZHANG Guan-cai , GUAN Lian-zhu.
2013.
Single and Combined Effects of As (III) and Acetochlor on Phosphatase Activity in Soil. Journal of Integrative Agriculture, 12(6): 1079-1086.
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