|
|
|
|
|
| Isolation and Structural Speculation of Herbicide-Active Compounds from the Metabolites of Pythium aphanidermatum |
| ZHANG Li-hui, ZHANG Jin-lin, LIU Ying-chao, CAO Zhi-yan, HAN Jian-min, YANG Juan , DONG Jingao |
| Mycotoxin and Molecular Plant Pathology Lab, Agricultural University of Hebei, Baoding 071001, P.R.China |
|
|
|
摘要 Natural herbicides, or environment-friendly bioherbicides have been attracted more and more attentions. Isolation and structural identification of natural herbicide-active compounds from plant pathogens has been proved to be an effective approach for novel lead discovery of the pesticide development. In this study, the metabolites of the mutant strain PAM1, which obtained from PA1 of Pythium aphanidermatum (Eds.) Fitzp by ultraviolet radiation were separated and identified by HPLC, NMR, and IR. The results revealed that three active compounds including 4-hydroxy-3-methoxycinnamic acid and two indole derivatives, exhibited inhibition activity on the elongation of radical and coleoptile of Digtaria sanguinalis (L.) Scop.
Abstract Natural herbicides, or environment-friendly bioherbicides have been attracted more and more attentions. Isolation and structural identification of natural herbicide-active compounds from plant pathogens has been proved to be an effective approach for novel lead discovery of the pesticide development. In this study, the metabolites of the mutant strain PAM1, which obtained from PA1 of Pythium aphanidermatum (Eds.) Fitzp by ultraviolet radiation were separated and identified by HPLC, NMR, and IR. The results revealed that three active compounds including 4-hydroxy-3-methoxycinnamic acid and two indole derivatives, exhibited inhibition activity on the elongation of radical and coleoptile of Digtaria sanguinalis (L.) Scop.
|
|
Received: 15 November 2012
Accepted:
|
| Fund: This research was supported by the grants from the National High Technology Research and Development Program of China (2011AA10A206) and the National Natural Science Foundation of China (31171877). |
|
Corresponding Authors:
Correspondence DONG Jin-gao, Tel: +86-312-7528266, Fax: +86-312-7528266, E-mail: dongjingao@126.com
E-mail: dongjingao@126.com
|
| About author: ZHANG Li-hui, E-mail: zhanglihui@hebau.edu.cn |
Cite this article:
ZHANG Li-hui, ZHANG Jin-lin, LIU Ying-chao, CAO Zhi-yan, HAN Jian-min, YANG Juan , DONG Jingao.
2013.
Isolation and Structural Speculation of Herbicide-Active Compounds from the Metabolites of Pythium aphanidermatum. Journal of Integrative Agriculture, 12(6): 1026-1032.
|
| [1]Ahmed E F, Yi H J. 2008. Production, partial purificationand characterization of feruloyl esterase by Aspergillusawamori in submerged fermentation. BiotechnologyJournal, 3, 1264-1275[2]Cherif M, Menzies J G, Ehret D L, Bogdanoff C, Belanger RR. 1994. Yield of cucumber infected with Pythiumaphanidermatum when grown with soluble silicon.HortScience, 29, 896-897[3]Gerwick B C, Fields S S, Graupner P R, Gray J A, Chapin E L,Cleveland J A, Heim D R. 1997. Pyridazocidin, a newmicrobial phytotoxin with activity in the mehler reaction.Weed Science, 45, 654-657[4]Faulds C B. 2010. What can feruloyl esterases do for us?Phytochemistry Reviews, 9, 121-132[5]Faulds C B, Aliwan F O. 1998. Chemical and thermal stabilityof ferulic acid (feruloyl) esterases from Aspergillus.Progress in Biotechnology, 15, 41-46[6]Kim W G, Kim J P, Park D J, Kim C J, Kwak S S, Yoo I D.1996. AB3217-A and B, herbicidal compounds relatedto anisomycin from Streptomyces sp. ME-13[7]Agricultural Chemistry and Biotechnology, 39, 153-158[8]Kim Y O K, Cho Y D. 2000. Growth inhibition in suspensionculturedsoybean cells in the presence of ferulic acid ismediated through spermine accumulation. Journal ofPlant Physiology, 156, 133-136[9]Klein K, Blum U. 1990. Inhibition of cucumber leafexpansion by ferulic acid in split-root experiments.Journal of Chemical Ecology, 16, 455-463[10]Lee H B, Kim C J, Kim J S, Hong K S, Cho K Y 2003. Ableaching herbicidal activity of methoxyhygromycin(MHM) produced by an actinomycete strainStreptomyces sp. 8E-12 Letters in AppliedMicrobiology, 36, 387-391[11]Li Y J, Chen S, Li C Y, Deng S J, Gu W X. 2008. Isolation andidentification of allelochemicals from Mikaniamicrantha. Journal of South China AgriculturalUniversity, 29, 26-30[12]Mastro G D, Fracchiolla M, Verdini L, Montemurro P. 2006.Oregano and its potential use as bioherbicide. ActaHorticulturae, 723, 335-345[13]Shimada A, Takeuchi S, Nakajima A, Tanaka S, Kawano T,Kimura Y. 1999. Phytotoxicity of indole-3-acetic acidproduced by the fungus, Pythium aphanidermatumBioscience Biotechnology and Biochemistry, 64, 187-189[14]Singh H P, Batish D R, Setia N, Kohli R K. 2005. Herbicidalactivity of volatile oils from Eucalyptus citriodoraagainst Parthenium hysterophorus. Annals of AppliedBiology, 146, 89-94[15]Topakas E, Kalogeris E. 2003. Production and partialcharacterisation of feruloyl esterase by Porotrichumthermophile in solid-state fermentation. ProcessBiochemistry, 38, 1539-1543[16]Valdes Rodriguez O A, Garcia Espinoza R, Sanchez SanchezO, Perez Vazquez A . 2011. Isolation and pathogenicityof a possible Pythium aphanidermatum in Jatrophacurcas L. non toxic. Tropical and SubtropicalAgroecosystems, 14, 649-660[17]Varkonda S. 1985. Herbicides of microbial origin [Bialaphos,Anisomycin, Metoxyphenone, Cycloheximide],Streptomyces spp. Agrochemia, 25, 171-174[18]Zhang L H, Kang Z H, Xu J, Xu W C, Zhang J L. 2010.Isolation and structural identification of herbicidal toxinfractions produced by Pythium aphanidermatum.Agricultural Sciences in China, 9, 101-105[19]Zhang L H, Zhang J L, Dong J G. 2009. Solid fermentationof Pythium aphanidermatum and separation ofherbicidal active substance. In: Proceedings of 2009International Conference of Natural Products andTraditional Medicine. Springer, Germany. pp. 146-150[20]Zhu Y Z, Qiang S. 2004. Isolation, pathogenicity and safetyof Curvularia eragrostidis isolate QZ-2000 as abioherbicide agent for large crabgrass (Digitariasanguinalis) Biocontrol Science and Technology, 14,769-782. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
